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81.
Near pH 2.0, lysozyme in water is in its native conformation, and in water/methanol (2/8) it adopts a helical denatured conformation (Kamatari et al. Protein Sci. 1998, 7, 681-688). Hydrogen/deuterium (H/D) exchange of lysozyme in solution confirms that it is partially unfolded at pH 2.0 in water/methanol (v/v = 2/8). With electrospray ionization (ESI) mass spectrometry (MS), lysozyme in water produces ions with charges +7 to +12, with the greatest intensity at +10, whereas lysozyme in water/methanol (2/8) produces ions with charges +6 to +12 with the greatest intensity at +7. Thus, lysozyme is an exception to the rule that a protein denatured in solution forms higher charge states than the same protein in its folded native conformations in solution. Because the same charge states are produced from these two solution conformations, a direct comparison of the properties of the gas-phase ions produced from two very different solution conformations is possible. The conformations of lysozyme ions in the gas phase were studied using cross section measurements and gas-phase H/D exchange. Similar cross sections and H/D exchange levels were observed for same-charge states of lysozyme ions formed from the native and helical denatured conformations in solution. Cross sections show that the ions have compact structures. Thus, disulfide-intact gaseous lysozyme ions generated from the denatured state in water/methanol (2/8) refold into compact structures in the gas phase on a time scale of milliseconds or less. 相似文献
82.
83.
Noting that frequency distributions of clearness indices available in the literature are not applicable for all climates, the present article examines the suitability of a new parameter,
, and a variable,
, to depict generalized clearness index distributions.
is a normalized monthly average daily clearness index, while
is a normalized daily clearness index. These normalized clearness indices comprise maximum and minimum daily clearness indices (KT,max and KT,min), in a month, in addition to monthly average daily clearness index,
, and daily clearness index, KT. Correlations have been developed to predict KT,max and KT,min needed, in the absence of data. The distributions, developed in terms of
and
have been suitably represented and tested over 70 locations, latitudes ranging from 8°N to 64°N. The daily clearness index values have been predicted within a standard deviation of 0.034 with the expressions developed in the present study. 相似文献
84.
A. Srirama Rao B. R. Phanikumar R. Dayakar Babu K. Suresh 《Canadian Metallurgical Quarterly》2007,133(5):531-538
Granular pile anchors (GPA) are one of the recent innovative foundation techniques devised for mitigating the problems posed by swelling clay beds. In a granular pile anchor, the footing is anchored to an anchor plate at the bottom of the granular pile. This makes the granular pile tension resistant and enables it to absorb the tensile force caused on the foundation by the swelling clay. An understanding of the amount of uplift resistance offered by the GPA is important in the design of granular pile-anchor foundations in field situations causing tensile forces on foundations, such as in expansive clay beds. This paper presents the results of a field-scale test program conducted to study the pullout response of GPAs embedded in expansive clay beds. Pullout load tests were conducted on GPAs of varying lengths and diameters. It was found from the field pullout load tests that granular pile anchors of larger surface area resulted in higher pullout capacity. Of the various single granular pile anchors with l/d values between 2.5 and 10, the GPA of length 1000?mm and diameter 200?mm (l/d = 5) showed the best pullout load response when tested alone, resulting in a failure uplift capacity of 14.71?kN. Increase in diameter and length of granular pile anchor increased the uplift capacity. When the length of the GPA was increased from 500 to 750 and 1000?mm, the percentage increase in the uplift load required for an upward movement of 25?mm was 33.3 and 55.5% respectively. The pullout load of the GPA when tested under group was 18?kN as against a 12?kN for the GPA when tested single. 相似文献
85.
Theofanis Strouboulis Lin Zhang Ivo Babuška 《International journal for numerical methods in engineering》2007,69(2):250-283
In this paper, we address the cost versus accuracy capabilities for the generalized FEM (GFEM) which was developed in (Comput. Methods Appl. Mech. Eng. 2003; 192 :3109–3161, Int. J. Numer. Meth. Engng 2004; 60 :1639–1672, Ph.D. Thesis, Texas A&M University, College Station, TX, August 2003 (Advisor: T. Strouboulis)), and also the construction of two‐sided a posteriori error estimates, which can be used to assess the achieved accuracy at all levels of the method. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献
86.
K. Saravana Kumar Ravindra Babu Misra 《国际自动化与计算杂志》2007,4(4):388-395
Software operational profile (SOP) is used in software reliability prediction,software quality assessment,performance analysis of software,test case allocation,determination of"when to stop testing,"etc.Due to the limited data resources and large efforts required to collect and convert the gathered data into point estimates,reluctance is observed by the software professionals to develop the SOP.A framework is proposed to develop SOP using fuzzy logic,which requires usage data in the form of linguistics.The resulting profile is named fuzzy software operational profile (FSOP).Based on this work,this paper proposes a generalized approach for the allocation of test cases,in which occurrence probability of operations obtained from FSOP are combined with the criticality of the operations using fuzzy inference system (FIS).Traditional methods for the allocation of test cases do not consider the application in which software operates.This is intuitively incorrect.To solve this problem,allocation of test cases with respect to software application using the FIS model is also proposed in this paper. 相似文献
87.
Babu P.N. Govind G. Prasad S.M.S. Bhat K.N. 《Device and Materials Reliability, IEEE Transactions on》2007,7(3):420-428
In this paper, we report the electrical characteristics and reliability studies on tunnel oxides fabricated by "wet N2O" oxidation of silicon in an ambient of water vapor and N2O at a furnace temperature of 800 degC. Tunnel oxides that have an equivalent oxide thickness of 67 A are subjected to a constant-current stress (CCS) amount of -100 mA/cm2 using a MOS capacitor to obtain information on stress-induced leakage current (SILC), interface, and bulk trap generation. The obtained results clearly demonstrate the superior performance features of the present tunnel oxides with reduced SILC, lower trap generation, minimum change in gate voltage, and higher charge-to-breakdown during CCS studies. X-ray photoelectron spectroscopy depth profile studies of the tunnel oxide interfaces have shown that the improved performance characteristics and reliability can be attributed to the incorporation of about 8.5% nitrogen at the oxide-silicon interface of the samples formed by the "wet N2O" process that involves low-temperature oxidation and annealing at 800 degC. 相似文献
88.
Non‐Volatile ReRAM Devices Based on Self‐Assembled Multilayers of Modified Graphene Oxide 2D Nanosheets
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Adila Rani Dhinesh Babu Velusamy Richard Hahnkee Kim Kyungwha Chung Filipe Marques Mota Cheolmin Park Dong Ha Kim 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(44):6167-6174
2D nanomaterials have been actively utilized in non‐volatile resistive switching random access memory (ReRAM) devices due to their high flexibility, 3D‐stacking capability, simple structure, transparency, easy fabrication, and low cost. Herein, it demonstrates re‐writable, bistable, transparent, and flexible solution‐processed crossbar ReRAM devices utilizing graphene oxide (GO) based multilayers as active dielectric layers. The devices employ single‐ or multi‐component‐based multilayers composed of positively charged GO (N‐GO(+) or NS‐GO(+)) with/without negatively charged GO(‐) using layer‐by‐layer assembly method, sandwiched between Al bottom and Au top electrodes. The device based on the multi‐component active layer Au/[N‐GO(+)/GO(‐)]n/Al/PES shows higher ON/OFF ratio of ≈105 with switching voltage of ?1.9 V and higher retention stability (≈104 s), whereas the device based on single component (Au/[N‐GO(+)]n/Al/PES) shows ≈103 ON/OFF ratio at ±3.5 V switching voltage. The superior ReRAM properties of the multi‐component‐based device are attributed to a higher coating surface roughness. The Au/[N‐GO(+)/GO(–)]n/Al/PES device prepared from lower GO concentration (0.01%) exhibits higher ON/OFF ratio (≈109) at switching voltage of ±2.0 V. However, better stability is achieved by increasing the concentration from 0.01% to 0.05% of all GO‐based solutions. It is found that the devices containing MnO2 in the dielectric layer do not improve the ReRAM performance. 相似文献
89.
B. Krishna Mohan B. Babu Madhavan U. M. Das Gupta 《International journal of remote sensing》2013,34(8):1709-1723
A methodology has been formulated to integrate images from IRS-1A LISS II of two dates for landuse/landcover classification. The methodology developed includes image classification by fuzzy k-means clustering and fusion of memberships by fuzzy set theoretic operators. The two date images have been geometrically coregistered and classified for the identification of land classes individually. The fuzzy memberships of the classified output images have been integrated by using fuzzy logic operators like algebraic sum and gamma (gamma) operator. The classification accuracy of the resultant land classes in the integrated images was verified with the ground data collected in situ. The resultant images have been evaluated by kappa (kappa) statistic and it was found that output from the image of fuzzy algebraic sum operator scored high in generating the land classes, with an overall accuracy of 95%. 相似文献
90.
This study attempted to quantify the variations of the surface marine atmospheric boundary layer (MABL) parameters associated with the tropical Cyclone Gonu formed over the Arabian Sea during 30 May–7 June 2007 (just after the monsoon onset). These characteristics were evaluated in terms of surface wind, drag coefficient, wind stress, horizontal divergence, and frictional velocity using 0.5° × 0.5° resolution Quick Scatterometer (QuikSCAT) wind products. The variation of these different surface boundary layer parameters was studied for three defined cyclone life stages: prior to the formation, during, and after the cyclone passage. Drastic variations of the MABL parameters during the passage of the cyclone were observed. The wind strength increased from 12 to 22 m s?1 in association with different stages of Gonu. Frictional velocity increased from a value of 0.1–0.6 m s?1 during the formative stage of the system to a high value of 0.3–1.4 m s?1 during the mature stage. Drag coefficient varied from 1.5 × 10?3 to 2.5 × 10?3 during the occurrence of Gonu. Wind stress values varied from 0.4 to 1.1 N m?2. Wind stress curl values varied from 10 × 10?7 to 45 × 10?7 N m?3. Generally, convergent winds prevailed with the numerical value of divergence varying from 0 to –4 × 10?5 s?1. Maximum variations of the wind parameters were found in the wall cloud region of the cyclone. The parameters returned to normally observed values in 1–3 days after the cyclone passage. 相似文献