Vitamin D and Death by Sunshine

Exposure to sunlight is the major cause of skin cancer. Ultraviolet radiation (UV) from the sun causes damage to DNA by direct absorption and can cause skin cell death. UV also causes production of reactive oxygen species that may interact with DNA to indirectly cause oxidative DNA damage. UV increases accumulation of p53 in skin cells, which upregulates repair genes but promotes death of irreparably damaged cells. A benefit of sunlight is vitamin D, which is formed following exposure of 7-dehydrocholesterol in skin cells to UV. The relatively inert vitamin D is metabolized to various biologically active compounds, including 1,25-dihydroxyvitamin D3. Therapeutic use of vitamin D compounds has proven beneficial in several cancer types, but more recently these compounds have been shown to prevent UV-induced cell death and DNA damage in human skin cells. Here, we discuss the effects of vitamin D compounds in skin cells that have been exposed to UV. Specifically, we examine the various signaling pathways involved in the vitamin D-induced protection of skin cells from UV.     

Reversible crosslinking of polymers bearing pendant or terminal thiol groups prepared by nitroxide-mediated radical polymerization

Monomers or N-alkoxyamine initiators containing protected thiol groups are utilized to prepare polymers via nitroxide-mediated radical polymerization. Following thiol deprotection, the macromolecular properties of these polymers are manipulated, by adjusting the redox conditions to either form or cleave disulfide bonds, or irreversibly cap free thiols by the rapid addition to a maleimide Michael acceptor. Formation of disulfide bonds under dilute conditions results in intramolecular disulfide formation, resulting in internal polymer collapse. Alternatively, disulfide formation under high concentration results in intermolecular crosslinking of polymers to form networked macromolecular assemblies.     

Theory of mind associations with other cognitive functions and brain imaging in normal aging.

The study investigated age-related differences in theory of mind and explored the relationship between this ability, other cognitive abilities, and structural brain measures. A cohort of 106 adults (ages 50–90 years) was recruited. Participants completed tests of theory of mind, verbal and performance intelligence, executive function, and information processing speed and underwent structural magnetic resonance imaging (measurement of whole brain volume, volume of white matter hyperintensities, and diffusion tensor imaging of white matter integrity). Theory of mind ability declined with increasing age, and the relationship between theory of mind and age was fully mediated by performance intelligence, executive function, and information processing speed and was partially mediated by verbal intelligence. Theory of mind performance correlated significantly with diffusion tensor imaging measures of white matter integrity but not with volume of white matter hyperintensities or whole-brain volume. Theory of mind age-related decline may not be independent of other cognitive functions; it may also be particularly susceptible to changes in white matter integrity. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Using a MODFLOW grid, generated with GMS, to solve a transport problem with TOUGH2 in complex geological environments: The intertidal deposits of the Venetian Lagoon

The tides of the Venetian Lagoon generally vary between −0.5 and +0.7 m asl. Occasionally, they may reach a maximum of 1.5 m (acqua alta) and a minimum of −0.8 m asl (acqua bassa). Intertidal areas, called “barene,” exist all along the coast of the Lagoon. These areas are characterized by canals that concentrate the flow of water (and the deposition of sands) during the rising and waning of the tides, and that inundate and drain the vegetated areas found between canals (where organic-rich clays are deposited). Therefore, since the area is subject to subsidence, in time, sand dykes (the original canals) become juxtaposed to clayey dykes (the original vegetated areas). In addition, the sands form a continuous hydrogeologic network within the clays, very similar to that of a vascular system that effectively drains the whole “barena” deposits. In order to be effective, measures for monitoring, confining, or remediating the transport of pollutants through this kind of environment must explicitly take into account the geologic complexity. The same complexity must be included in the numerical models that support remediation efforts. At the moment, there appears to be no off-the-shelf graphical interface that is able to manage such complexity for TOUGH2. To attempt to solve this problem we have used a calibrated USGS-MODFLOW model, of the barena of “Passo a Campalto” in the Venetian Lagoon, developed with the GMS graphical interface. The model is made of 42 layers, which, apart from the first layer, are 0.5 m thick; the first layer has the thickness distribution of a dump found on top of the barena deposit at Passo a Campalto. Each layer consists of 100×60 square cells, for a total of 252,000 cells, only about half of which are active. Using a FORTRAN routine, we translate this grid, with all the hydrogeologic boundary conditions, into a TOUGH2 input file, and we provide the additional necessary information for running a TOUGH2 simulation. The results are promising, in that we were able to produce TOUGH2 grids with very complex geology and to run the models with success. For visualization, the results can be imported back into GMS as 3D scatter point sets, or they can be plotted with any adequate plotting software such as MatLab. Developing conceptual and numerical models with an elaborate graphical interface such as GMS effectively allows setting up complex problems while concentrating on their physics.     

Isotopic and Elemental Authenticity Markers: a Case Study on Cypriot Wines

This study monitors variations in isotopes and elements in relation to grape variety, environmental factors and provenance in order to address the wine authenticity issue. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) assessed the wines’ elemental content. Site-specific nuclear isotope fractionation-nuclear magnetic resonance and isotope ratio mass spectrometry methodologies determined in authentic and commercial wines the distribution of the naturally occurring stable isotopes of the deuterium/hydrogen (D/H) ratios and carbon (¹³C/¹²C) in ethanol of wine and oxygen ratio (¹⁸O/¹⁶O) in wine water. Chemometrics delineated the elements and isotopes responsible for wine classification. Specifically, unsupervised principal component analysis (PCA) framed the importance of grape variety and provenance, while supervised analysis pinpointed the vineyard effect and highlighted the contribution of the vintage year. Validation steps ensured that the extracted models do not predict randomly and their results are reliable. In fact, the acquired results can be incorporated to the EU Wine Isotopic Databank database providing both a guide and a tool for eventual candidatures for denomination of origin and support both Cypriot wine and winemakers. In this context, this research contributes to authenticity assurance of wines and adds value to final products, while it helps controlling hazards arising from environmental changes.     

A study of diffusion in poly(ethyleneglycol)-gelatin based semi-interpenetrating networks for use in wound healing

Semi-interpenetrating networks (sIPNs) designed to mimic extracellular matrix via covalent crosslinking of poly(ethylene glycol) diacrylate in the presence of gelatin have been shown to aid in wound healing, particularly when loaded with soluble factors. Ideal systems for tissue repair permit an effective release of therapeutic agents and flow of nutrients to proliferating cells. Appropriate network characterization can, consequently, be used to convey an understanding of the mass transfer kinetics necessary for materials to aid in the wound healing process. Solute transport from and through sIPNs has not yet been thoroughly evaluated. In the current study, the diffusivity of growth factors and nutrients through the polymeric system was determined. Transport of keratinocyte growth factor was modeled by treating the sIPN as a plane sheet into which the protein was loaded. The diffusion coefficient was determined to be 4.86 × 10⁻⁹ ± 1.86 × 10⁻¹² cm²/s. Glucose transport was modeled as flow through a semi-permeable membrane. Using lag-time analysis, the diffusion coefficient was calculated to be 2.25 × 10⁻⁶ ± 1.98 × 10⁻⁷ cm²/s. The results were evaluated in conjunction with previous studies on controlled drug release from sIPNs. As expected from Einstein-Stokes equation, diffusivity decreased as molecular size increased. The results offer insight into the structure-function design paradigm and show that release from the polymeric system is diffusion controlled, rather than dissolution controlled.