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991.
Thermophiles as Potential Source of Novel Endotoxin Antagonists: the Full Structure and Bioactivity of theLipo‐oligosaccharide from Thermomonas hydrothermalis
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Dr. Flaviana Di Lorenzo Dr. Ida Paciello Dr. Luigi Lembo Fazio Dr. Luciana Albuquerque Dr. Luisa Sturiale Prof. Milton S. da Costa Prof. Rosa Lanzetta Prof. Michelangelo Parrilli Prof. Domenico Garozzo Prof. Maria Lina Bernardini Dott. Alba Silipo Prof. Antonio Molinaro 《Chembiochem : a European journal of chemical biology》2014,15(14):2146-2155
Thermomonas hydrothermalis is a Gram‐negative thermophilic bacterium that is able to live at 50 °C. This ability is attributed to chemical modifications, involving those to bacterial cell‐wall components, such as proteins and (glyco)lipids. As the main component of the outer membrane of Gram‐negative bacteria, lipopolysaccharides (LPSs) are exposed to the environment, thus they can undergo structural chemical changes to allow thermophilic bacteria to live at their optimal growth temperature. Furthermore, as one of the major target of the eukaryotic innate immune system, LPS elicits host immune response in a structure‐dependent mode; thus the uncommon chemical features of thermophilic bacterial LPSs might exert a different biological action on the innate immune system—an antagonistic effect, as shown in studies of LPS structure–activity relationship in the ongoing research into antagonist LPS candidates. Here, we report the complete structural and biological activity analysis of the lipo‐oligosaccharide isolated from Thermomonas hydrothermalis, achieved by a multidisciplinary approach (chemical analysis, NMR, MALDI MS and cellular immunology). We demonstrate a tricky and interesting structure combined with a very interesting effect on human innate immunity. 相似文献
992.
Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP‐Dependent Ca2+ Translocation by Sarco‐Endoplasmic Reticulum Ca2+‐ATPase (SERCA)
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Fabrizio‐Zagros Sadafi Lara Massai Dr. Gianluca Bartolommei Prof. Maria Rosa Moncelli Prof. Luigi Messori Dr. Francesco Tadini‐Buoninsegni 《ChemMedChem》2014,9(8):1660-1664
Sarco‐endoplasmic reticulum Ca2+‐ATPase (SERCA), a P‐type ATPase that sustains Ca2+ transport and plays a major role in intracellular Ca2+ homeostasis, represents a therapeutic target for cancer therapy. Here, we investigated whether ruthenium‐based anticancer drugs, namely KP1019 (indazolium [trans‐tetrachlorobis(1H‐indazole)ruthenate(III)]), NAMI‐A (imidazolium [trans‐tetrachloro(1H‐imidazole)(S‐dimethylsulfoxide)ruthenate(III)]) and RAPTA‐C ([Ru(η6‐p‐cymene)dichloro(1,3,5‐triaza‐7‐phosphaadamantane)]), and cisplatin (cis‐diammineplatinum(II) dichloride) might act as inhibitors of SERCA. Charge displacement by SERCA adsorbed on a solid‐supported membrane was measured after ATP or Ca2+ concentration jumps. Our results show that KP1019, in contrast to the other metal compounds, is able to interfere with ATP‐dependent translocation of Ca2+ ions. An IC50 value of 1 μM was determined for inhibition of calcium translocation by KP1019. Conversely, it appears that KP1019 does not significantly affect Ca2+ binding to the ATPase from the cytoplasmic side. Inhibition of SERCA at pharmacologically relevant concentrations may represent a crucial aspect in the overall pharmacological and toxicological profile of KP1019. 相似文献
993.
Ícaro L. J. da Silva André L. F. de Almeida Robert Baldemair Sorour Falahati F. Rodrigo P. Cavalcanti 《Wireless Personal Communications》2014,75(4):2523-2547
In 3rd generation partnership project (3GPP) long term evolution (LTE) systems, when no resources has been assigned in the uplink to a given user equipment (UE), the control information associated with layers 1 and 2 in the protocol stack is conveyed back to the base station through the so-called physical uplink control channel (PUCCH). In PUCCH, the data streams transmitted by multiple UEs are multiplexed in the time-domain and in the frequency-domain with the aid of spreading codes. Although the spreading codes associated with UEs within the same cell can be assumed to be orthogonal, the presence of inter-cell interference (ICI) in multi-cell scenarios severely limits receiver performance. In particular, the Format 1 of PUCCH, which is associated with the transmission of hybrid automatic repeat request acknowledgements (ACK/NACK) and scheduling requests, has a major impact on system performance, since an incorrectly decoded ACK/NACK message may introduce significant delay in data transmission. In this contribution, we propose a new multi-user receiver for ICI reduction in PUCCH LTE that operates both in cooperative and non-cooperative multi-cell architectures. The proposed receiver relies on a constrained tensor modeling of the received signal in PUCCH signaling, and affords an iterative joint channel estimation and symbol detection by simultaneously exploiting the energy of the data symbols and the pilot tones present in PUCCH. The formulation of the proposed algebraic receiver model incorporates symbol-basis hopping and slot-basis hopping signaling schemes, which are interference randomization techniques existing in the 3GPP specifications of LTE system. Computer simulation results show the remarkable performance gains of the proposed receiver compared to the conventional time-frequency decorrelator based receiver. 相似文献
994.
Pedro M. P. Salomé Paulo A. Fernandes Joaquim P. Leitão Marta G. Sousa Jennifer P. Teixeira António F. da Cunha 《Journal of Materials Science》2014,49(21):7425-7436
In this work, we present the Raman peak positions of the quaternary pure selenide compound Cu \(_2\) ZnSnSe \(_4\) (CZTSe) and related secondary phases that were grown and studied under the same conditions. A vast discussion about the position of the X-ray diffraction (XRD) reflections of these compounds is presented. It is known that by using XRD only, CZTSe can be identified but nothing can be said about the presence of some secondary phases. Thin films of CZTSe, Cu \(_2\) SnSe \(_3\) , ZnSe, SnSe, SnSe \(_2\) , MoSe \(_2\) and a-Se were grown, which allowed their investigation by Raman spectroscopy (RS). Here we present all the Raman spectra of these phases and discuss the similarities with the spectra of CZTSe. The effective analysis depth for the common back-scattering geometry commonly used in RS measurements, as well as the laser penetration depth for photoluminescence (PL) were estimated for different wavelength values. The observed asymmetric PL band on a CZTSe film is compatible with the presence of CZTSe single-phase and is discussed in the scope of the fluctuating potentials' model. The estimated bandgap energy is close to the values obtained from absorption measurements. In general, the phase identification of CZTSe benefits from the contributions of RS and PL along with the XRD discussion. 相似文献
995.
Eduardo M. Szesz Gelson B. de Souza Gabriel G. de Lima Bruno A. da Silva Neide K. Kuromoto Carlos M. Lepienski 《Journal of materials science. Materials in medicine》2014,25(10):2265-2275
The combination of shot blasting (SB) and micro-arc oxidation (or anodic oxidation—AO) in titanium surfaces was shown to provide enhanced conditions for cell differentiation and osseointegration than those provided by SB or AO alone. This study associated both methods aiming to attain titania layers on Ti with adequate tribo-mechanical features for bone implants. SB was performed using alumina particles, and titania layers were grown by AO using a CaP-based electrolyte. Mechanical properties and scratch resistance were characterized at nanoscale by instrumented indentation and nanoscratch, and correlated with morphological and microstructural changes (XRD, SEM, EDS, AFM, and profilometry). Analytical methods were employed to correct roughness and substrate effects on the indentation results. CaP-containing TiO2 layers were produced on AO and SB + AO. The latter presented small pore size and inhomogeneous layer thickness and Ca/P ratios, caused by the non-uniform surface straining by SB that affects the oxide growth kinetics in the electrochemical process. Elastic modulus of SB + AO layer (37 GPa) were lower than the AO one (45 GPa); both of them were smaller than bulk Ti (130 GPa) and close to bone values. The hardness profiles of AO and SB + AO were similar to the substrate ones. Because of the improved load bearing capacity and unique layer features, the critical load to remove the SB + AO titania coating in scratch tests was three times as much or higher than in AO. Results indicate improved mechanical biocompatibility and tribological strength of anodic titania layers grown on sand blasted Ti surfaces. 相似文献
996.
Laura Mayol Daniela De Stefano Virginia Campani Francesca De Falco Eleonora Ferrari Claudia Cencetti Pietro Matricardi Luigi Maiuri Rosa Carnuccio Angela Gallo Maria Chiara Maiuri Giuseppe De Rosa 《Journal of materials science. Materials in medicine》2014,25(6):1483-1493
In this study, a sterile and biocompatible chitosan (CHI) gel for wound healing applications was formulated. CHI powder was treated in autoclave (ttCHI) to prepare sterile formulations. The heat treatment modified the CHI molecular weight, as evidenced by GPC analysis, and its physical–chemical features. Differential scanning calorimetry studies indicated that the macromolecules, before and after thermal treatment, differ in the strength of water-polymer interaction leading to different viscoelastic and flow properties. Thermally treated CHI exhibited the following effects: (i) increased the proliferation and migration of human foreskin foetal fibroblasts at 24 h; (ii) accelerated wound healing (measured as area of lesion) at 3 and 10 days in an in vivo model of pressure ulcers. These effects were linked to the increase of the hydroxyproline and haemoglobin content as well as Wnt protein expression. Moreover, we found a reduction of myeloperoxidase activity and TNF-α mRNA expression. These observations suggest the potential of this novel CHI gel in wound healing and other therapeutic applications. 相似文献
997.
Natalia C. V. Melo Rosa M. A. Moyses Rosilene M. Elias Manuel C. M. Castro 《Hemodialysis international. International Symposium on Home Hemodialysis》2014,18(2):473-480
There are no studies evaluating the impact of dialyzer reprocessing on solute removal in short‐daily online hemodiafiltration (OL‐HDF). Our aim was to evaluate the impact of dialyzer reuse on solute removal in daily OL‐HDF and compare with that in high‐flux short‐daily hemodialysis (SDH). Fourteen patients undergoing a SDH program were included. Pre‐dialysis and post‐dialysis blood samples and effluent dialysate were collected in the 1st, 7th, and 13th dialyzer uses in SDH sessions and in daily OL‐HDF sessions. Directly quantified small solute (urea, phosphorus, creatinine, and uric acid) total mass removal (TMDQ) and clearance (KDQ) were similar when the 1st, 7th, and 13th dialyzer SDH uses were compared with the 1st, 7th, and 13th daily OL‐HDF uses. TMDQ and KDQ of small solutes were similar among analyzed dialyzer uses in SDH sessions and in daily OL‐HDF sessions. β2‐Microglobulin TMDQ and KDQ were statistically higher in daily OL‐HDF dialyzer uses than in the respective SDH uses. There was no difference in β2‐microglobulin TMDQ and KDQ among dialyzer uses in daily OL‐HDF sessions or in SDH sessions. In daily OL‐HDF, albumin loss was significantly different among dialyzer uses (P < 0.001), being lower in the 7th and 13th dialyzer uses than in the first use. Dialyzer reprocessing did not impair solute extraction in daily OL‐HDF. β2‐Microglobulin removal was greater in daily OL‐HDF than in SDH sessions, without significant differences in other solutes extraction. There was a significant reduction in intradialytic albumin loss with dialyzer reprocessing in daily OL‐HDF sessions. 相似文献
998.
Paula Gregorio‐Godoy Jorge Pérez‐Juste Luis M. Liz‐Marzán Beatriz H. Juárez J. Ricardo Arias‐Gonzalez 《Small (Weinheim an der Bergstrasse, Germany)》2014,10(2):376-384
Temperature changes in the vicinity of a single absorptive nanostructure caused by local heating have strong implications in technologies such as integrated electronics or biomedicine. Herein, the temperature changes in the vicinity of a single optically trapped spherical Au nanoparticle encapsulated in a thermo‐responsive poly(N‐isopropylacrylamide) shell (Au@pNIPAM) are studied in detail. Individual beads are trapped in a counter‐propagating optical tweezers setup at various laser powers, which allows the overall particle size to be tuned through the phase transition of the thermo‐responsive shell. The experimentally obtained sizes measured at different irradiation powers are compared with average size values obtained by dynamic light scattering (DLS) from an ensemble of beads at different temperatures. The size range and the tendency to shrink upon increasing the laser power in the optical trap or by increasing the temperature for DLS agree with reasonable accuracy for both approaches. Discrepancies are evaluated by means of simple models accounting for variations in the thermal conductivity of the polymer, the viscosity of the aqueous solution and the absorption cross section of the coated Au nanoparticle. These results show that these parameters must be taken into account when considering local laser heating experiments in aqueous solution at the nanoscale. Analysis of the stability of the Au@pNIPAM particles in the trap is also theoretically carried out for different particle sizes. 相似文献
999.
Nanoelectronics is a very promising step the world of electronics is taking. It is proved to be more efficient than the microelectronic approaches currently in use, mainly in terms of area and energy management. A Single-Electron Transistor (SET) is capable of confining electrons to sufficiently small dimensions, so that the quantization of both their charge and their energy is easily observable, making the SET's quantum mechanical devices. These features should allow building chips with a number of devices orders of magnitude greater than indicated by the roadmap still respecting area and power consumption restrictions. In this sense, Tera Scale Integrated (TSI) systems can be feasible in the future. A digital module, such as an arithmetic logic unit, completely implemented with SETs has already been proposed and validated by simulation. In this work a completely SET based network-on-chip (NoC) nanoelectronic core is proposed. Furthermore, a simple NoC architecture based on that nanoelectronic core is also evaluated. It is shown that the SET-based NoC has a promising performance considering parameters such as power consumption, area and clock frequency. A simple comparison of mesh NoC chip prototypes is shown. 相似文献
1000.
Hybrid Organic/Inorganic Nanostructures for Highly Sensitive Photoelectrochemical Detection of Dissolved Oxygen in Aqueous Media
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Sebastiano Bellani Ali Ghadirzadeh Laura Meda Alberto Savoini Alessandra Tacca Gianluigi Marra Rui Meira Jorge Morgado Fabio Di Fonzo Maria Rosa Antognazza 《Advanced functional materials》2015,25(28):4531-4538
Precise, reliable, and remote measurement of dissolved oxygen in aqueous media is of great importance for many industrial, environmental, and biological applications. In particular, photoelectrochemical sensors working in differential mode have recently demonstrated promising properties, in terms of stability, sensitivity, and application potential. Here, a new approach is presented, combining visible light sensitivity, efficient photocurrent generation, and solution‐processed fabrication methods of conjugated polymers, with charge carriers selectivity, energetic alignment favorable to efficient interfacial charge transfer and high surface area achievable by using metal oxide nanostructures. Extensive characterization and optimization of the hybrid organic/inorganic system are carried out, leading to the realization of an oxygen sensor device, based on nanostructured palladium oxide/poly[(9,9‐dioctylfluorenyl‐2,7‐diyl)‐alt‐5,5‐(4′,7′‐di‐2‐thienyl‐2′,1′,3′‐benzothiadiazole]/[6,6]phenyl‐C61‐butyric acid methyl ester (PdO/APFO‐3:PCBM) as materials of choice. State‐of‐the‐art sensitivity, amounting at ?5.87 μA cm?2 ppm?1, low background signal, in the order of ?4.85 μA cm?2, good electrochemical stability for more than 2 h of continuous functioning and high reproducibility of the signal over the pH 1 to 10 range, are reported, making the hybrid device suitable for several practical uses. The results fully validate the mixed organic/inorganic approach for photoelectrochemical applications, and pave the way for its further exploitation in fields like waste water treatment, environmental monitoring, and water splitting. 相似文献