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81.
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83.
Sílvia C. Rodrigues Renato M. S. Cardoso Patricia C. Freire Cludia F. Gomes Filipe V. Duarte Ricardo Pires das Neves Joana Simes-Correia 《International journal of molecular sciences》2021,22(18)
Umbilical cord blood (UCB) has long been seen as a rich source of naïve cells with strong regenerative potential, likely mediated by paracrine signals. More recently, small extracellular vesicles (sEV), such as exosomes, have been shown to play essential roles in cell-to-cell communication, via the transport of numerous molecules, including small RNAs. Often explored for their potential as biomarkers, sEV are now known to have regenerative and immunomodulating characteristics, particularly if isolated from stem cell-rich tissues. In this study, we aim to characterize the immunomodulating properties of umbilical cord blood mononuclear cell-derived sEV (UCB-MNC-sEV) and explore their therapeutic potential for inflammatory skin diseases. UCB-MNC-sEV were shown to shift macrophages toward an anti-inflammatory phenotype, which in turn exert paracrine effects on fibroblasts, despite previous inflammatory stimuli. Additionally, the incubation of PBMC with UCB-MNC-sEV resulted in a reduction of total CD4+ and CD8+ T-cell proliferation and cytokine release, while specifically supporting the development of regulatory T-cells (Treg), by influencing FOXP3 expression. In a 3D model of psoriatic skin, UCB-MNC-sEV reduced the expression of inflammatory and psoriatic markers IL6, IL8, CXCL10, COX2, S100A7, and DEFB4. In vivo, UCB-MNC-sEV significantly prevented or reversed acanthosis in imiquimod-induced psoriasis, and tendentially increased the number of Treg in skin, without having an overall impact on disease burden. This work provides evidence for the anti-inflammatory and tolerogenic effect of UCB-MNC-sEV, which may be harnessed for the treatment of Th17-driven inflammatory skin diseases, such as psoriasis. 相似文献
84.
Dr. Ricardo Ferraz Prof. João Costa‐Rodrigues Prof. Maria H. Fernandes Dr. Miguel M. Santos Dr. Isabel M. Marrucho Prof. Luís Paulo N. Rebelo Prof. Cristina Prudêncio Prof. João Paulo Noronha Dr. Željko Petrovski Dr. Luís C. Branco 《ChemMedChem》2015,10(9):1480-1483
Significant antiproliferative effects against various tumor cell lines were observed with novel ampicillin salts as ionic liquids. The combination of anionic ampicillin with appropriate ammonium, imidazolium, phosphonium, and pyridinium cations yielded active pharmaceutical ingredient ionic liquids (API‐ILs) that show potent antiproliferative activities against five different human cancer cell lines: T47D (breast), PC3 (prostate), HepG2 (liver), MG63 (osteosarcoma), and RKO (colon). Some API‐ILs showed IC50 values between 5 and 42 nM , activities that stand in dramatic contrast to the negligible cytotoxic activity level shown by the ampicillin sodium salt. Moreover, very low cytotoxicity against two primary cell lines—skin (SF) and gingival fibroblasts (GF)—indicates that the majority of these API‐ILs are nontoxic to normal human cell lines. The most promising combination of antitumor activity and low toxicity toward healthy cells was observed for the 1‐hydroxyethyl‐3‐methylimidazolium–ampicillin pair ([C2OHMIM][Amp]), making this the most suitable lead API‐IL for future studies. 相似文献
85.
Rodolfo Daniel Velasco-Barraza Ricardo Vera-Graziano Eduardo Alberto López-Maldonado Mercedes Teresita Oropeza-Guzmán Syed G. Dastager Adriana Álvarez-Andrade 《国际聚合物材料杂志》2018,67(13):800-807
In recent times, electrospun nanofibers have been widely studied from several biotechnological approaches; in this work, poly(acrylic acid) (PAA) solutions mixed with chitosan and alginate were electrospun and characterized to determine the behavior of these fibers when used in combination with bacteria, different samples were incubated with the bacterial strains: Streptomyces spp., Micromonospora spp., and Escherichia coli and a OD600 test was performed. The formation of nanofibers via electrospinning and the physicochemical properties of the obtained fibers were evaluated. Results showed that the presence of chitosan enhanced the thermal stability of PAA, since PAA/alginate fibers lost 5% of their mass at 41°C, whereas PAA/chitosan lost this amount at around 125°C. The fibers demonstrated suitable characteristics to be used as a bacteria bioreactor. 相似文献
86.
87.
Alejandro Moncayo-Lasso Ricardo A. Torres-Palma John Kiwi Norberto Benítez Csar Pulgarin 《Applied catalysis. B, Environmental》2008,84(3-4):577-583
A woven inorganic silica fabric loaded with Fe-ions (EGF-Fe) was tested under simulated solar light during hydroquinone degradation. The abatement of hydroquinone was observed to attain about 80% within 3 h. The photo-catalyst was also tested to inactive Escherichia coli K12 at “natural” pH and in the presence of a low concentration of H2O2. Addition of H2O2 (10 mg L−1) did not by itself to bacterial inactivation. Total bacterial inactivation was mediated by EGF-Fe fabrics in the presence of H2O2 (10 mg L−1) under solar light irradiation. A sample containing active (culturable) bacteria (105 CFU mL−1) decreased to values <1 CFU mL−1 within 3 h reaction. Fe-mediated homogeneous process decreased the bacterial CFU content by about two orders of magnitude within 4 h. During the degradation of hydroquinone only a small amount of iron ions were found in solution of about 1.2 mg L−1, within 90 min decreasing to values ≤0.5 mg L−1 after 180 min. The leaching of Fe-ions did not affect the photo-catalyst performance since EGF-Fe fabric did not deactivate after five or more cycles. The Fe-ions founds in solution mineralized hydroquinone to levels below 37% of its initial content. The present study presents the first report on the beneficial role of a heterogeneous iron supported catalyst leading to efficient bacterial inactivation in aqueous solution with iron leaching <0.1 mg L−1, the detection limit for Fe-analysis in solution. No bacterial re-growth was observed during a post-irradiation period up to 24 h in the dark. 相似文献
88.
Ricardo Acosta Ortiz Aida Esmeralda Garcia Valdez Gustavo Soria Arguello Guadalupe Mendez Padilla Omar Acosta Berlanga 《Journal of Polymer Research》2018,25(7):160
Herein a method is described to prepare photocurable thermally-conductive shape memory epoxy/ graphene composites. By photopolymerizing the epoxy resin diglycidyl ether of bisphenol A with an allyl-functionalized ditertiary amine as the curing agent, jointly with a multifunctional thiol, a crosslinked polyether-polythioether co-network was obtained. The presence of a soft domain like the flexible polythioethers enable the co-network to display shape memory properties. By varying the polyether to polythioether ratio it was possible to modulate the shape memory characteristics of the composite. The effect of the concentration of graphene nanoplatelets (GNP) in the composite was also investigated. Shape memory performances revealed excellent values of shape recovery and shape fixity with maximums of 98 and 99% respectively. The temporary- shaped composites with higher concentration of polythioethers and GNP regained their permanent shapes in 2–3 s when heated above the programming temperature. The thermal conductivity in the composites reached 0.39 W/m°K for the composite with 15% w/w of GNP. The presence of the polythioethers in the co-network enhanced the toughness of the composite as revealed by the impact resistance analysis. 相似文献
89.
Oliveira TW Leandro CG de Jesus Deiró TC dos Santos Perez G da França Silva D Druzian JI Couto RD Barreto-Medeiros JM 《Lipids》2011,46(11):1071-1074
The main goal of the present study was to evaluate the long-term effects of a perinatal palatable high-fat diet on the food
intake and cholesterol profile of adult rats. Male Wistar rats (aged 22 days) were divided into two groups according to their
mother’s diet during gestation and lactation (C
p, n = 10; pups from control mothers; and HLp
n = 10; pups from mothers fed a palatable high-fat diet). At the 76th day, pups were housed individually for 14 days, and daily
food consumption was determined during a period of 6 days. Blood from 100-day-old rats was sampled by cardiac puncture. Fasting
(12 h) serum glucose, total cholesterol, LDL-C, HDL-C, triglycerides (TG), and VLDL-C levels were determined. The measurement
of food intake was higher in the animals submitted to a hyperlipidic diet during the perinatal period. Serum total cholesterol,
LDL-C, HDL-C, TG, VLDL-C and glycemia were increased in the HLp group compared to the control group. Our findings show that an early life environment with a high-fat diet can contribute
to metabolic disease in later life. 相似文献
90.
Edouard Chauveau Catherine Marestin Régis Mercier Alexis Brunaux Vincent Martin Ricardo P. Nogueira Aurélien Percheron Virginie Roche Hugues Waton 《应用聚合物科学杂志》2015,132(17)
As a part of research work to elaborate polymeric materials for metal corrosion protection, we have developed a new family of phosphonic acid‐containing polymers. The synthesis and the characterization of polysulfones bearing alkyl phosphonate ester side groups are first described. These polymers are synthesized by direct polycondensation of a phosphonate ester‐containing bisphenol by aromatic nucleophilic substitution. The physicochemical properties of the resulting polymers are described. Acidic hydrolysis of phosphonate esters results in the formation of phosphonic acid groups. A series of phosphonic acid‐containing polysulfones is therefore obtained and characterized. A preliminary evaluation of the anticorrosive properties of these polymers is described. In 0.25M Na2SO4 solution, the corrosion rate of a polymer‐coated mild steel sample is much lower than of the free metal substrate. These results suggest that phosphonic acid‐containing polysulfones might be interesting as anticorrosive coatings. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 41890. 相似文献