Synthesis and release of ATP by soluble mitochondrial F1 in complex with its inhibitor protein during dimethylsulfoxide-water transitions

Soluble mitochondrial F1 and F1 in complex with the natural ATPase inhibitor protein (F1-IP) catalyze the spontaneous synthesis of [gamma-32P]ATP from medium [32P]phosphate and enzyme-bound ADP when incubated in media with dimethylsulfoxide (Me2SO); under these conditions, the synthesized [gamma-32P]ATP is not released into the media, it remains tightly bound to the enzymes [Gómez-Puyou, A., Tuena de Gómez-Puyou, M. & de Meis, L. (1986) Eur. J. Biochem. 159, 133-140]. Some of the characteristics of the synthesized [gamma-32P]ATP were studied in F1 and F1-IP (ATPase activities of 70 and 1-3 micromol x min(-1) x mg(-1), respectively). In Me2SO media, gamma-phosphate of synthesized ATP in F1 or F1-IP exchanges with medium phosphate. From the rates of the exchange reaction, the half-times for hydrolysis of the synthesized ATP in F1 and F1-IP were calculated: 45 min and 58 min for F1 and F1-IP, respectively. The course that synthesized [gamma-32P]ATP follows after dilution of the Me2SO synthetic mixture with aqueous buffer was determined. After dilution, the half-life of synthesized ATP in F1 was less than 1 min. In F1-IP, ATP was also hydrolyzed, but at significantly lower rates. In F1-IP, dilution also produced release of the synthesized [gamma-32P]ATP. This was assayed by the accessibility of [gamma-32P]ATP to hexokinase. About 25% of [gamma-32P]ATP synthesized in F1-IP, but not in F1, was released into the media after dilution with aqueous buffer that contained 20 mM phosphate. Release of tightly bound ATP required the binding energy of phosphate and solvation of F1-IP, however, the particular kinetics of F1-IP were also central for medium ATP synthesis in the absence of electrochemical H+ gradients.     

Optimization of the properties obtained by quenching in martensitic stainless steels X30-40Cr13 and X40-60CrMoV14

An experimental study of the effects produced by different parameters of the heat-quenching cycle permitted the optimization of the properties typical of these martensitic stainless steels: hardness and corrosion resistance. The results obtained demonstrate that the heating temperatures currently used in industrial quenching treatment of these steels should be modified.     

HIV-1 and HIV-2 antibodies in pregnant women in the City of Maputo, Mozambique. A comparative study between 1982/1983 and 1990

The seroprevalence of HIV-1 and HIV-2 among pregnant women in Maputo, the capital of Mozambique, was compared between the years 1982/83, 1988 and 1990. None of the 432 serum samples collected in 1982/83 was positive for HIV antibodies whereas in 1988 the HIV-1 and HIV-2 seroprevalence was 0.4% (2/500) and 0.6% (3/500), respectively, and in 1990 0.6% (12/2014) and 0.2% (4/2014), respectively. These results indicate that HIV infection has been introduced recently in Maputo and is spreading at a slow rate among women.     

Spatial soliton-induced guiding by cross-phase modulation

The authors show that spatial solitons are able to induce the stable guiding of a weak probe beam in a homogeneous Kerr-type nonlinear media through cross-phase modulation (XPM). The modes of the induced waveguide are derived from the propagation equation of the probe. Experiments have been performed in Kerr liquid CS₂, with beams of picosecond duration at infrared and visible wavelengths. Stable guiding of a green probe induced by an IR soliton beam has been demonstrated in several different experimental situations. Induced antiguiding and beam splitting are also reported for pump and probe of perpendicular polarizations. All results are in excellent agreement with numerical simulations of the propagation     

Measurement of synchronous machine rotor angle from analysis ofzero sequence harmonic components of machine terminal voltage

Generator rotor angle measurement is useful in identification of electric machine parameters in the d and q axes and as an indicator of stability of the operating condition. Rotor angle measurements with the appropriate bandwidth are also very useful in observation of rotor torsional oscillations both for monitoring and protection against undamped torsional modes, and for use in control schemes designed to damp torsional oscillations. A method of measurement of synchronous machine rotor angle from an analysis of the phase angle of zero sequence harmonic components of terminal voltage is presented     

Ischemia‐reperfusion injury. A phenomenon,that also occurs in the bone

Ischemia‐reperfusion injury of the bone occurs due to traumatic and non‐traumatic alterations affecting blood supply to the bone. It is likely to occur also upon insertion of an implant. Ischemia‐reperfusion injury of the bone has been studied by interruption of blood supply in situ, in limb replantation/transplantation models, in revascularized bone grafts and non‐vascularized bone fragments, as well as in isolated cultured cells. All cells of the bone are affected, including osteoblasts, osteocytes, osteoclasts, chondrocytes, and bone marrow cells. Critical ischemia times for induction of bone cell death, either in the ischemic period or following reperfusion, are in the range of 3 to 7 h. These critical ischemia times are significantly increased by decreasing the temperature from 37 °C to 0–4 °C. Anoxia is the most likely trigger of cell injury in the ischemic phase. In the reperfusion phase, reactive oxygen species are decisively involved in the injurious process. In general, however, the available information on the mechanism of ischemia‐reperfusion injury of the bone is relatively sparse. On the other hand, there are clear similarities to the mechanisms of ischemia‐reperfusion injury known from other organs, and there is a clear potential for protection against ischemia‐reperfusion injury of the bone.     

Flood risk mapping at European scale.

The aim of this article is to illustrate a framework for flood risk mapping at pan-European scale produced by the Weather-Driven Natural Hazards (WDNH) action of the EC-JRC-IES. Early results are presented in the form of flood risk index maps. We assess several flood risk factors that contribute to the occurrence of flood disasters. Among the causal factors of a flood disaster one is triggering a natural event in the form of extreme precipitation and consequently extreme river discharge and extreme flood water levels. The threatening natural event represents the hazard component in our assessment. Furthermore exposure and vulnerability are anthropogenic factors that contribute also to flood risk. In the proposed approach, flood risk is considered on the light of exposure, vulnerability and hazard. We use a methodology with a marked territorial approach for the assessment of the flood risk. Hence, based on mathematical calculations, risk is the product of hazard, exposure and vulnerability. Improvements on datasets availability and spatial scale are foreseen in the next phases of this study. This study is also a contribution to the discussion about the need for communication tools between the natural hazard scientific community and the political and decision making players in this field.     

Joint Effect of MLSE and Receiver Filters Optimization on Dispersion Robustness of IMDD, DPSK, DQPSK, and Duobinary Modulation

We analyze the effectiveness of a 32-state maximum-likelihood sequence-estimation (MLSE) receiver on chromatic dispersion robustness of optical transmission based on several binary modulation formats: intensity modulation direct detection, differential phase-shift keying, and duobinary line coding. Multilevel differential quadrature phase-shift keying modulation is also analyzed using a four-state 2-bit/symbol joint MLSE processor. For all modulation formats, receiver filters are optimized together with the use of the MLSE technique.