Enantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones

Enantioselectivity of enzyme catalysis is often rationalizedvia active site models. These models are constructed on thebasis of comparing the enantiomeric excess of product observedin a series of reactions which are conducted with a range ofhomologous substrates, typically carrying various side chainsubstitutions. Surprisingly the practical application of thesesimple but informative `pocket size' models has been rarelytested in genetic engineering experiments. In this paper wereport the construction, purification and enantioselectivityof two recombinant Rhizomucor miehei lipases which were designedto check the validity of such a model in reactions of ring openingof oxazolin-5(4H)-ones.     

Recovery and grade prediction of pilot plant flotation column concentrate by a hybrid neural genetic algorithm

Today flotation column has become an acceptable means of froth flotation for a fairly broad range of applications, in particular the cleaning of sulfides. Even after having been used for several years in mineral processing plants, the full potential of the flotation column process is still not fully exploited. There is no prediction of process performance for the complete use of available control capabilities. The on-line estimation of grade usually requires a significant amount of work in maintenance and calibration of on-stream analyzers, in order to maintain good accuracy and high availability. These difficulties and the high cost of investment and maintenance of these devices have encouraged the approach of prediction of metal grade and recovery. In this paper, a new approach has been proposed for metallurgical performance prediction in flotation columns using Artificial Neural Network (ANN). Despite of the wide range of applications and flexibility of NNs, there is still no general framework or procedure through which the appropriate network for a specific task can be designed. Design and structural optimization of NNs is still strongly dependent upon the designer’s experience. To mitigate this problem, a new method for the auto-design of NNs was used, based on Genetic Algorithm (GA). The new proposed method was evaluated by a case study in pilot plant flotation column at Sarcheshmeh copper plant. The chemical reagents dosage, froth height, air, wash water flow rates, gas holdup, Cu grade in the rougher feed, flotation column feed, column tail and final concentrate streams were used to the simulation by GANN. In this work, multi-layer NNs with Back Propagation (BP) algorithm with 8-17-10-2 and 8-13-6-2 arrangements have been applied to predict the Cu and Mo grades and recoveries, respectively. The correlation coefficient (R) values for the testing sets for Cu and Mo grades were 0.93, 0.94 and for their recoveries were 0.93, 0.92, respectively. The results discussed in this paper indicate that the proposed model can be used to predict the Cu and Mo grades and recoveries with a reasonable error.     

An embedded optical tracking system for motion-corrected magnetic resonance imaging at 7T

Object

Prospective motion correction using data from optical tracking systems has been previously shown to reduce motion artifacts in MR imaging of the head. We evaluate a novel optical embedded tracking system.

Materials and methods

The home-built optical embedded tracking system performs image processing within a 7T scanner bore, enabling high speed tracking. Corrected and uncorrected in vivo MR volumes are acquired interleaved using a modified 3D FLASH sequence, and their image quality is assessed and compared.

Results

The latency between motion and correction of the slice position was measured to be (19?±?5)?ms, and the tracking noise has a standard deviation no greater than 10???m/0.005° during conventional MR scanning. Prospective motion correction improved the edge strength by 16?% on average, even though the volunteers were asked to remain motionless during the acquisitions.

Conclusion

Using a novel method for validating the effectiveness of in vivo prospective motion correction, we have demonstrated that prospective motion correction using motion data from the embedded tracking system considerably improved image quality.     

Anti-Windup Compensator Designs for Nonsalient Permanent-Magnet Synchronous Motor Speed Regulators

This paper presents and compares a number of anti-windup compensator designs which address the problem of current saturation within high-performance permanent-magnet synchronous motor applications. The compensator variants include an integrator reset scheme, back calculation and tracking, and an optimally synthesized low-order dynamic compensator. Performance is compared first through simulation tests using a nonlinear nonsalient machine model with multirate discrete time control loops. Second, the results of tests repeated on a hardware implementation are shown. In both simulation and experiment, the back calculation and tracking and low-order designs are shown to exhibit clear performance advantages over the reset strategy.     

Parallelizing a GIS on a shared address space architecture

We are developing a high-performance GIS (our term for a parallel GIS) on an SGI Challenge, a 16-processor machine with a shared address space architecture (SASA). We describe how we parallelized a key GIS operation using a message-passing algorithm. We advocate the linking of two diverse approaches to the design of parallel architectures and algorithms. As part of our project, we evaluated the effect of parallelizing an important GIS operation: range query. We parallelized a range query using data partitioning (to reduce synchronization) and dynamic load balancing (to improve speedup). We found that these approaches do achieve the performance required for many GIS applications     

Psychological aspects of severe burn injuries in children.

Discusses 4 phases of the traumatic process associated with severe burn injuries in children. Predisposing factors (i.e., emotional disturbances) in the preinjury phase are discussed. The acute phase centers on treatment directed toward physiological stabilization. The intermediate phase, in which skin grafts and other painful medical procedures are performed, is characterized by dependency on adults and regression, behavior problems, and such emotional reactions as blaming the parents. Strategies for preparing children for and helping them cope with pain are discussed. The rehabilitative phase often involves difficulties in social readjustment. Strategies for dealing with each phase are presented, most of which focus on prevention efforts. (PsycINFO Database Record (c) 2010 APA, all rights reserved)