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Two purified animal venom toxins, crotoxin and cardiotoxin, have been combined to produce a unique natural product (VRCTC-310) currently under investigation as an antitumor agent by the National Cancer Institute. In vitro, it has demonstrated cytotoxic disease specificity and a unique mechanism of action when submitted to COMPARE analysis. In vivo, tolerance was developed to the neurotoxic properties of crotoxin which allowed comparison of several schedules of fixed and escalating daily i.m. doses to mice bearing s.c. Lewis Lung carcinoma. An 83% inhibition of tumor growth was achieved using an escalating dose schedule starting at 1.8 mg/kg and reaching 6.3 mg/kg/day on day 20. Although some irritation around the sites of i.m. injection was noted, animal weight loss was negligible and there were no other signs of adverse toxicity. This natural product represents a new, membrane interactive anticancer agent which produces a unique spectrum of cytotoxicity in vitro and which has demonstrated interesting in vivo antitumor efficacy.  相似文献   
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We compared the data from four growth hormone (GH) immunoassays for analyzing 24-h GH profiles in four apparently normal subjects and four obese subjects (508 serum samples). The detection limit was 0.02 microgram/L for one immunochemiluminometric assay (ICMA), 0.1 microgram/L for two IRMAs, and 0.4 microgram/L for one RIA. All GH pulses with a peak ICMA value > 1 microgram/L were detected by each of the other methods. Overall, the correlation coefficient between the values obtained with all four assays exceeded 0.90. However, for GH concentrations < or = 0.25 microgram/L, acceptable concordance (r2 > or = 0.80) was reached only between the ICMA and one IRMA; between the ICMA and the RIA, concordance was acceptable only for GH concentrations > or = 10 micrograms/L. In the normal subjects, the percentage of undetectable values was 0% with the ICMA but 29% with one of the IRMAs; in obese subjects, the corresponding values were 12% and 38%.  相似文献   
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A concept is proposed for utilizing artificial neural networks to enhance the high-speed tracking accuracy of robotic manipulators. Tracking accuracy is a function of the controller's ability to compensate for disturbances produced by dynamical interactions between the links. A model-based control algorithm uses a nominal model of those dynamical interactions to reduce the disturbances. The problem is how to provide accurate dynamics information to the controller in the presence of payload uncertainty and modeling error. Neural network payload estimation uses a series of artificial neural networks to recognize the payload variation associated with a degradation in tracking performance. The network outputs are combined with a knowledge of nominal dynamics to produce a computationally efficient direct form of adaptive control. The concept is validated through experimentation and analysis on the first three links of a PUMA-560 manipulator. A multilayer perceptron architecture with two hidden layers is used. Integration of the principles of neural network pattern recognition and model-based control produces a tracking algorithm with enhanced robustness to incomplete dynamic information. Tracking efficacy and applicability to robust control algorithms are discussed.  相似文献   
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A method of approximate channel identification is proposed that is based on a simplification of the correlation estimator. Despite the numerical simplification (no multiplications or additions are required, only comparisons and an accumulator), the performance of the proposed estimator is not significantly worse than that of the standard correlation estimator. A free (user selectable) parameter moves ‘smoothly’ from a situation with small sum‐squared channel estimation error but hard‐to‐identify channel peaks, to one with a larger sum‐squared estimation error but easy‐to‐identify channel peaks. The proposed estimator is shown to be biased and its behaviour is analysed in a number of situations. Applications of the proposed estimator to sparsity detection, symbol timing recovery and to the initialization of blind equalizers are suggested. Copyright © 2002 John Wiley & Sons, Ltd.  相似文献   
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Any intelligent problem solving system should be able, given the known data on a case, to decide whether some item of information is true, false or unknown. In this paper the way in which various forms of commonsense reasoning can be integrated to provide such decisions is described. To this end three structural types of knowledge defined over data, and four strategies for exploiting these structures, are identified. ‘Decide-Status’ integrates the reasoning strategies into a task frame. This frame structure not only integrates the reasoning but also affords the appropriate facilities for providing strategic justifications for its conclusions, if required.  相似文献   
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Wastewater treatment technologies suitable for serving large populations are generally reliable and reasonably cost-effective, yet they are almost always financially inappropriate for small communities (< 2,000 p.e.). Comparative cost data suggests that waste stabilization ponds should be an attractive option for small communities, yet perceptions relating to land costs, climate and effluent quality have limited their application in the UK. This paper details typical UK land costs, climate and winter performance data for a pilot-scale waste stabilization pond with various upgrading technologies: system A, two tertiary maturation ponds in series; B, two tertiary maturation ponds in series followed by a reed bed channel; C, a control rock filter; D, an aerated rock filter; and E, a constructed wetland. System D was found to perform best, closely followed by system B.  相似文献   
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