The microsomal triglyceride transfer protein catalyzes the post-translational assembly of apolipoprotein B-100 very low density lipoprotein in McA-RH7777 cells

In cells in which the lipoprotein assembly process had been inactivated by brefeldin A (BFA), membrane-associated apoB-100 disappeared without forming lipoproteins or being secreted, indicating that it was degraded. Reactivation of the assembly process by chasing the cells in the absence of BFA, gave rise to a quantitative recovery of the membrane-associated apoB-100 in the very low density lipoprotein (VLDL) fraction in the medium. These results indicate that the membrane-associated apoB-100 can be converted to VLDL. A new method was developed by which the major amount (88%) of microsomal apoB-100 but not integral membrane proteins could be extracted. The major effect of this method was to increase the recovery of apoB-100 that banded in the LDL and HDL density regions, suggesting that the membrane-associated form of apoB-100 is partially lipidated. We also investigated the role of the microsomal triglyceride transfer protein (MTP) in the assembly of apoB-100 VLDL using a photoactivatable MTP inhibitor (BMS-192951). This compound strongly inhibited the assembly and secretion of apoB-100 VLDL when present during the translation of the protein. To investigate the importance of MTP during the later stages in the assembly process, the cells were preincubated with BFA (to reversibly inhibit the assembly of apoB-100 VLDL) and pulse-labeled (+BFA) and chased (+BFA) for 30 min to obtain full-length apoB-100 associated with the microsomal membrane. Inhibition of MTP after the 30-min chase blocked assembly of VLDL. This indicates that MTP is important for the conversion of full-length apoB-100 into VLDL. Results from experiments in which a second chase (-BFA) was introduced before the inactivation of MTP indicated that only early events in this conversion of full-length apoB-100 into VLDL were blocked by the MTP inhibitor. Together these results indicate that there is a MTP-dependent "window" in the VLDL assembly process that occurs after the completion of apoB-100 but before the major amount of lipids is added to the VLDL particle. Thus the assembly of apoB-100 VLDL from membrane-associated apoB-100 involves an early MTP-dependent phase and a late MTP-independent phase, during which the major amount of lipid is added.     

A note on the differential regulator equation for non-minimum phase linear systems with time-varying exosystems

Some control problems in practice are often formulated as a linear output regulation problem with time-varying exosystems. Although this problem has been studied recently, an explicit and constructive solution has been given only for minimum phase systems. This paper presents a solution for non-minimum phase systems whose zero dynamics is hyperbolic (or has an exponentially dichotomic split in the case of time-varying zero dynamics). The idea is inspired by the non-causal stable inversion.     

A novel iterative shape from focus algorithm based on combinatorial optimization

Shape from focus (SFF) is a technique to estimate the depth and 3D shape of an object from a sequence of images obtained at different focus settings. In this paper, the SFF is presented as a combinatorial optimization problem. The proposed algorithm tries to find the combination of pixel frames which produces maximum focus measure computed over pixels lying on those frames. To reduce the high computational complexity, a local search method is proposed. After the estimate of the initial depth map solution of an object, the neighborhood is defined, and an intermediate image volume is generated from the neighborhood. The updated depth map solution is found from the intermediate image volume. This update process of the depth map solution continues until the amount of improvement is negligible. The results of the proposed SFF algorithm have shown significant improvements in both the accuracy of the depth map estimation and the computational complexity, with respect to the existing SFF methods.     

A new vertical‐alignment LC mode with high‐transmittance and fast‐response‐time features

Abstract— A novel pixel design for vertical‐alignment LCDs with superior transmittance has been developed. The new liquid‐crystal mode, refered to as the hole‐induced vertical‐alignment mode (Hi‐VA), uses a via hole of an organic layer on a TFT substrate to achieve multi‐domain alignment. Compared to the conventional design, the Hi‐VA mode has a transmittance of up to 135% with a contrast ratio of 2000:1. Moreover, the new structure is free from ITO patterning or protrusion on the color‐filter side, which makes the fabrication process simple and low cost.     

A two-stage heuristic for single machine capacitated lot-sizing and scheduling with sequence-dependent setup costs

This paper considers a single machine capacitated lot-sizing and scheduling problem. The problem is to determine the lot sizes and the sequence of lots while satisfying the demand requirements and the machine capacity in each period of a planning horizon. In particular, we consider sequence-dependent setup costs that depend on the type of the lot just completed and on the lot to be processed. The setup state preservation, i.e., the setup state at the end of a period is carried over to the next period, is also considered. The objective is to minimize the sum of setup and inventory holding costs over the planning horizon. Due to the complexity of the problem, we suggest a two-stage heuristic in which an initial solution is obtained and then it is improved using a backward and forward improvement method that incorporates various priority rules to select the items to be moved. Computational tests were done on randomly generated test instances and the results show that the two-stage heuristic outperforms the best existing algorithm significantly. Also, the heuristics with better priority rule combinations were used to solve case instances and much improvement is reported over the conventional method as well as the best existing algorithm.     

Chaotic exploration and learning of locomotion behaviors

We present a general and fully dynamic neural system, which exploits intrinsic chaotic dynamics, for the real-time goal-directed exploration and learning of the possible locomotion patterns of an articulated robot of an arbitrary morphology in an unknown environment. The controller is modeled as a network of neural oscillators that are initially coupled only through physical embodiment, and goal-directed exploration of coordinated motor patterns is achieved by chaotic search using adaptive bifurcation. The phase space of the indirectly coupled neural-body-environment system contains multiple transient or permanent self-organized dynamics, each of which is a candidate for a locomotion behavior. The adaptive bifurcation enables the system orbit to wander through various phase-coordinated states, using its intrinsic chaotic dynamics as a driving force, and stabilizes on to one of the states matching the given goal criteria. In order to improve the sustainability of useful transient patterns, sensory homeostasis has been introduced, which results in an increased diversity of motor outputs, thus achieving multiscale exploration. A rhythmic pattern discovered by this process is memorized and sustained by changing the wiring between initially disconnected oscillators using an adaptive synchronization method. Our results show that the novel neurorobotic system is able to create and learn multiple locomotion behaviors for a wide range of body configurations and physical environments and can readapt in realtime after sustaining damage.     

Time-of-flight sensor and color camera calibration for multi-view acquisition

This paper presents a multi-view acquisition system using multi-modal sensors, composed of time-of-flight (ToF) range sensors and color cameras. Our system captures the multiple pairs of color images and depth maps at multiple viewing directions. In order to ensure the acceptable accuracy of measurements, we compensate errors in sensor measurement and calibrate multi-modal devices. Upon manifold experiments and extensive analysis, we identify the major sources of systematic error in sensor measurement and construct an error model for compensation. As a result, we provide a practical solution for the real-time error compensation of depth measurement. Moreover, we implement the calibration scheme for multi-modal devices, unifying the spatial coordinate for multi-modal sensors. The main contribution of this work is to present the thorough analysis of systematic error in sensor measurement and therefore provide a reliable methodology for robust error compensation. The proposed system offers a real-time multi-modal sensor calibration method and thereby is applicable for the 3D reconstruction of dynamic scenes.     

J resistance behavior in functionally graded materials using cohesive zone and modified boundary layer models

This paper describes elastic–plastic crack growth resistance simulation in a ceramic/metal functionally graded material (FGM) under mode I loading conditions using cohesive zone and modified boundary layer (MBL) models. For this purpose, we first explore the applicability of two existing, phenomenological cohesive zone models for FGMs. Based on these investigations, we propose a new cohesive zone model. Then, we perform crack growth simulations for TiB/Ti FGM SE(B) and SE(T) specimens using the three cohesive zone models mentioned above. The crack growth resistance of the FGM is characterized by the J-integral. These results show that the two existing cohesive zone models overestimate the actual J value, whereas the model proposed in the present study closely captures the actual fracture and crack growth behaviors of the FGM. Finally, the cohesive zone models are employed in conjunction with the MBL model. The two existing cohesive zone models fail to produce the desired K–T stress field for the MBL model. On the other hand, the proposed cohesive zone model yields the desired K–T stress field for the MBL model, and thus yields J _R curves that match the ones obtained from the SE(B) and SE(T) specimens. These results verify the application of the MBL model to simulate crack growth resistance in FGMs.