N-methyl-2-hydroxyethylammonium oleate ionic liquid performance as corrosion inhibitor for mild steel in hydrochloric acid medium

The aim of the present study is to evaluate the performance of N-methyl-2-hydroxyethylammonium oleate ([m-2HEA][Ol]) as a corrosion inhibitor for mild steel in a 0.1-mol/L hydrochloric acid solution and also investigate the role of chloride in the inhibition mechanism. This protic ionic liquid (PIL) has formerly shown a high efficiency as a corrosion inhibitor in a neutral chloride medium. Electrochemical and weight loss measurements, surface contact angle determination, scanning electron microscopy, and Raman spectroscopy were used to understand the factors that influence the response of the studied inhibitor. Results revealed that [m-2HEA][Ol] behaves as a mixed-type adsorption inhibitor, by blocking cathodic sites and by modifying the activation energy of the anodic reaction, and it can reach up to 94–97% of inhibition efficiency. PIL adsorption was enhanced by the excess of positive charge of the mild steel. The effect of inhibitor molecule has been discussed to propose a mechanism that explains the inhibitory action of the corrosion inhibitor, pointing out the role of chloride in the inhibition mechanism.     

Serum Proteome in a Sporadic Amyotrophic Lateral Sclerosis Geographical Cluster

This study is meant to characterize the serum proteome in a small geographical cluster of sporadic ALS subjects originating from a restricted geographical area and sharing the same environmental exposure, in a broader context of evaluating the relevance of environmental factors to disease onset, status, and progression. An Artificial Neural Network based software is used to compare the relative abundance of proteins identified as different (by means of bi‐dimensional electrophoresis and mass spectrometry) in the serum proteome of patients and age‐matched healthy controls. The patient's group is characterized by altered levels of acute phase reactants and of proteins involved in lipid homeostasis, along with over‐representation of the APOE*4 allele. Characterization of the serum proteome in a small cluster of sporadic ALS patients, originating from a geographically restricted area with a high prevalence of the disease and evaluation of the results with software based on artificial neural networks, highlights the association of the relative abundance of some proteins (most notably, acute phase reactants and lipid homeostasis proteins) with the disease presence and status.     

Bonding ability of self-adhesive resin-cements after dentin biomodification with hyaluronic acid

Purpose: The present study evaluated the influence of the hyaluronic acid (HA) on the bonding ability of self-adhesive resin cements to dentin regarding the bond strength. Eighty bovine incisors were ground flat to obtain a 2-mm thick slices which received conical preparations. The specimens were randomly distributed into 4 groups (n = 15) according to the dentin pretreatment (1 – control: untreated dentin; 2 – application of HA) and the evaluation time (1 – control: immediate evaluation; 2 – hydrolytic degradation: 6 months of storage in water at 37 °C). Preparations received the application of a self-adhesive resin cement (RelyX U200 or MaxCem Elite). Push-out bond strength test was conducted (0.5 mm/min). The bond strength data was submitted to two-way ANOVA/Tukey’s test (α = 0.05). For U200, no significance was observed when comparing the immediate (24 h) and 6 months means for the control groups (unexposed specimens). Previous application of HA to dentin significantly reduced the bond strength of U200 to dentin in both evaluation times (p < 0.05). HA had no significant influence on the push-out bond strength means for the cement MAX in both evaluation times (p > 0.05). The type 1 failure mode (adhesive mode) occurred in 100% of the specimens, irrespective of the dentin treatment or evaluation times. Pretreatment of dentin with HA produces a material-dependent influence on the push-out bond strength. The bonding ability of RelyX U200 is negatively influenced by the pretreatment of dentin with HA, whereas the biomodification of dentin with this bioactive agent causes no impact for the cement MaxCem Elite.     

Quality assessment of bed sediments of the Po River (Italy)

Comprehensive and contemporary evaluations of physical, chemical and toxicological endpoints have been performed on bed sediments of the Po River, the major Italian watercourse. Two extensive sampling campaigns were conducted in summer and winter low-flow conditions. Composite sediment samples were collected from ten reaches of the main river: the first was located in the upper region (ambient control), and the others downstream of the confluences of nine principal tributaries. The two sampling programs were paralleled by contemporary investigations on the macroinvertebrate community. The particle-size composition along the Po River showed a relatively uniform distribution of fine sand, a progressive downstream decrease of coarse sands and a corresponding increase of fine materials. The levels of polychlorinated biphenyls (PCB), polycyclic aromatic hydrocarbons (PAH), extractable organo halides (EOX), Cd, Cr, Cu, Hg, Ni, Pb and Zn were determined in sediment fine particles (< 63 microm), and showed marked changes across the ten river reaches. Their longitudinal trends, as those of organic carbon and total nitrogen, were very similar and largely independent of the survey season. Sediment quality benchmarks were used to evaluate sediment chemistry, and, although the overall level of contamination was from moderate to low, the reaches located downstream of the tributaries Dora Riparia, Dora Baltea, Lambro and Oglio were considered to be at risk. Sediments were tested for toxicity on Oncorhynchus mykiss, Ceriodaphnia dubia, Raphidocelis subcapitata and Vibrio fischeri. The toxicity tests were conducted both with sediment extracts and whole samples. Sediment extracts showed toxic potentials that were consistent with the spatial distribution of contaminants. Whole-sediment toxicity showed moderate/low effects which also included false positives and negatives. Alterations of the macroinvertebrate community were found for many kilometers downstream of Dora Riparia, and with a seasonal dependence, also in other reaches of the Italian river. Principal component analysis (PCA) was used to describe the longitudinal and temporal changes of the Po River, and allowed the selection of the most useful and discriminating indicators.     

ANXA1 Contained in EVs Regulates Macrophage Polarization in Tumor Microenvironment and Promotes Pancreatic Cancer Progression and Metastasis

The tumor microenvironment (TME) is a dynamic system where nontumor and cancer cells intercommunicate through soluble factors and extracellular vesicles (EVs). The TME in pancreatic cancer (PC) is critical for its aggressiveness and the annexin A1 (ANXA1) has been identified as one of the oncogenic elements. Previously, we demonstrated that the autocrine/paracrine activities of extracellular ANXA1 depend on its presence in EVs. Here, we show that the complex ANXA1/EVs modulates the macrophage polarization further contributing to cancer progression. The EVs isolated from wild type (WT) and ANXA1 knock-out MIA PaCa-2 cells have been administrated to THP-1 macrophages finding that ANXA1 is crucial for the acquisition of a protumor M2 phenotype. The M2 macrophages activate endothelial cells and fibroblasts to induce angiogenesis and matrix degradation, respectively. We have also found a significantly increased presence of M2 macrophage in mice tumor and liver metastasis sections previously obtained by orthotopic xenografts with WT cells. Taken together, our data interestingly suggest the relevance of ANXA1 as potential diagnostic/prognostic and/or therapeutic PC marker.     

Energy use in two Italian and Chinese tile manufacturers: A comparison using an enterprise input–output model

It is becoming evident that sustainable development methods are now being considered by governments and corporations from all countries at both local and global level. Firms are increasingly looking to strike a balance between profit, preserving the local environment, and energy savings, rather than just looking for profit. In this paper, an enterprise input–output analysis based on production processes is used to investigate two tile manufacturers, one located in China and the other in Italy. The aim of the paper is to describe their energy/materials use and consequent pollution/waste emissions, and to make a comparison between them. As this industry uses huge quantities of energy, it is useful to monitor these consumption uses along with pollution levels. This accounting can help in planning future local development strategies that would make production cycles more efficient and sustainable.     

Ultrastructure of multicellular dwarf males with external gametangium in Oedogonium macrandrium (Oedogoniales,Chlorophyta)

This is the first comprehensive ultrastructural study on dwarf males with external gametangia in the ge in the genus Oedogonium, from androspore germination to the liberation of mature male gametes. The ultrastructure of the process in O. macrandrium Wittrok is similar to that of Bulbochaete hiloensis (Nordstedt) Tiffany, butwith two remarkable differences. In O. macrandrium : 1) instead of a true transverse wall, only condensed mucilage appears between the gametes of each antheridial cell, and 2) the cell wall between the basal cell and the basal most antheridial cell has simple plasmodesmata similar to those present in the transverse walls of vegetative cells, which are absent in B. hiloensis.