The noneffect of a large linear hydrocarbon, squalene, on the phosphatidylcholine packing structure

The interaction of squalene with liposomes and monolayers of dipalmitoyl phosphatidylcholine (DPL) has been studied by differential scanning calorimetry, Raman spectroscopy, and surface potential measurements. Mole ratios of squalene to DPL up to 9 to 1 were studied. In contrast to small, nonpolar molecules, which profoundly influence the structure of lipid bilayers as detected by changes in both their thermodynamic phase transition parameters and membrane fluidity, this large, nonpolar, linear hydrocarbon is devoid of such influences. It is clear from our data that a large nonpolar molecule such as squalene, having no polar group that might anchor it to the aqueous interface, cannot intercalate between the acyl chains either below or above the phase transition of DPL. This behavior is not compatible with models that treat the bilayer interior as a bulk hydrocarbon, and suggests that great caution should be exercised in extrapolating partition coefficients based on bulk hydrocarbon measurements to lipid bilayers.     

Modification of the effects of prior residence in xiphophorus (Pisces, Poeciliidae): the role of isolation and differences in size

Laboratory experiments on pairs of male male of the genus Xiphophorus were carried out to test the hypothesis that the duration of a pre-experimental isolation period would modify the effects of prior residence on aggressive behaviour and dominance. Resident and intruder were isolated either both for 3 or both for 22 h (Exp. 1), or one for 3 and other other for 22 h (Exp. 2). Prior residence effects were only evident when the resident had been isolated for 3 h. Other connections between isolation conditions, reciprocal aggression and final dominance were also analysed, as was the influence of size differences between the opponents.     

Effects of PKA and PKC on miniature excitatory postsynaptic currents in CA1 pyramidal cells

Protein kinases play an important role in controlling synaptic strength at excitatory synapses on CA1 pyramidal cells. We examined the effects of activating cAMP-dependent protein kinase or protein kinase C (PKC) on the frequency and amplitude of miniature excitatory postsynaptic currents (mEPSCs) with perforated patch recording techniques. Both forskolin and phorbol-12,13-dibutryate (PDBu) caused a large increase in mEPSC frequency, but only PDBu increased mEPSC amplitude, an effect that was not observed when standard whole cell recording was performed. These results support biochemical observations indicating that PKC, similar to calcium/calmodulin-dependent protein kinase II, has an important role in controlling synaptic strength via modulation of AMPA receptor function, potentially through the direct phosphorylation of the GluR1 subunit.     

The value of profundaplasty in the management of severe femoropopliteal occlusion

The author, the fifth superintendent of Alabama's Bryce Hospital since the Wyatt v. Stickney order was issued in 1972, discusses the major problems faced by an administrator in complying with the court-set standards. They relate to qualified mental health professionals, staffing ratios, staff attitudes, individualized treatment plans, and the hospital's negative image. He also discusses the role of the human rights committee and the hospital's goal of providing treatment of sufficient quality that the court order will be lifted.     

Antitumour activity and schedule dependency of 8-chloroadenosine-3'',5''-monophosphate (8-ClcAMP) against human tumour xenografts

We have mapped the chromosomal locations of three human nuclear genes for putative components of the apparatus of mitochondrial gene expression, using a combination of in situ hybridization and interspecies hybrid mapping. The genes RPMS12 (mitoribosomal protein S12, a conserved protein component of the mitoribosomal accuracy center), TUFM (mitochondrial elongation factor EF-Tu), and AFG3L1 (similar to the yeast genes Afg3 and Rca1 involved in the turnover of mistranslated or misfolded mtDNA-encoded polypeptides) were initially characterized by a combination of database sequence analysis, PCR, cloning, and DNA sequencing. RPMS12 maps to chromosome 19q13.1, close to the previously mapped gene for autosomal dominant hearing loss DFNA4. The TUFM gene is located on chromosome 16p11.2, with a putative pseudogene or variant (TUFML) located very close to the centromere of chromosome 17. AFG3L1 is located on chromosome 16q24, very close to the telomere. By virtue of their inferred functions in mitochondria, these genes should be regarded as candidates of disorders sharing features with mitochondrial disease syndromes, such as sensorineural deafness, diabetes, and retinopathy.     

Mirror fixation

A mirror is the ideal fixation device for an ophthalmoscopic examination: it is the essense of simplicity, and there is nothing the patient would rather watch than himself.