Kidney-Specific CAP1/Prss8-Deficient Mice Maintain ENaC-Mediated Sodium Balance through an Aldosterone Independent Pathway

The serine protease prostasin (CAP1/Prss8, channel-activating protease-1) is a confirmed in vitro and in vivo activator of the epithelial sodium channel ENaC. To test whether proteolytic activity or CAP1/Prss8 abundance itself are required for ENaC activation in the kidney, we studied animals either hetero- or homozygous mutant at serine 238 (S238A; Prss8^cat/+ and Prss8^cat/cat), and renal tubule-specific CAP1/Prss8 knockout (Prss8^PaxLC1) mice. When exposed to varying Na⁺-containing diets, no changes in Na⁺ and K⁺ handling and only minor changes in the expression of Na⁺ and K⁺ transporting protein were found in both models. Similarly, the α- or γENaC subunit cleavage pattern did not differ from control mice. On standard and low Na⁺ diet, Prss8^cat/+ and Prss8^cat/cat mice exhibited standard plasma aldosterone levels and unchanged amiloride-sensitive rectal potential difference indicating adapted ENaC activity. Upon Na⁺ deprivation, mice lacking the renal CAP1/Prss8 expression (Prss8^PaxLC1) exhibit significantly decreased plasma aldosterone and lower K⁺ levels but compensate by showing significantly higher plasma renin activity. Our data clearly demonstrated that the catalytic activity of CAP1/Prss8 is dispensable for proteolytic ENaC activation. CAP1/Prss8-deficiency uncoupled ENaC activation from its aldosterone dependence, but Na⁺ homeostasis is maintained through alternative pathways.     

球销装配线的平衡分析

从人机料法环测角度出发,采用工业工程方法和技术对球销总成装配线的平衡进行了分析,找到了生产时间浪费的各个节点,针对这些节点提出了合理的措施,最终达到了提高生产效率的目的。     

锂离子电池用无溶剂干法电极的制备及其性能研究

采用无溶剂电极制备技术成功制备了锂离子电池用LiNi0.8Co0.1Mn0.1O2干法电极片,采用扫描电子显微镜(SEM)和X射线能谱仪(EDS)对干法电极片的形貌和元素分布进行了分析测试,通过倍率充放电、交流阻抗、循环充放电等测试手段研究了干法电极片的电化学性能.结果表明:纤维状PTFE广泛地分布在LiNi0.8Co...     

定量荧光分析技术在原油性质判别方面的应用探讨——以胜利油区为例

该文以胜利油区的应用为例,从定性、定量的角度讨论了定量荧光分析技术在原油性质判别方面的作用。利用定量荧光仪输出的谱图特征、最大峰值及对应的波长位置可以定性地判别储层的原油性质,利用油性指数则可以实现对原油性质的较为准确的定量判别。     

横向交流磁场频率对堆焊金属组织及性能的影响

在镍基堆焊合金等离子弧堆焊过程中引入横向交流脉冲磁场,研究了横向交流脉冲磁场频率对等离子弧堆焊层金属组织及性能的影响,并利用光学金相、X射线衍射、显微硬度和湿砂橡胶轮磨损试验等方法,系统分析了不同脉冲磁场频率作用下堆焊试样的硬度、耐磨性及组织.结果表明,外加横向交流脉冲磁场可以有效改善堆焊层金属的结晶形态,细化晶粒,在适当的脉冲磁场频率作用下,可以获得最佳的电磁搅拌效果,增加堆焊层金属中硬质相的数量,控制硬质相的生长方向,提高等离子弧堆焊层的硬度和耐磨性.     

草铵膦铵盐的高效液相色谱测定

建立了用高效液相色谱分析草铵膦铵盐的方法.使用Nucleosio SB离子交换柱,柱温室温;以0.1 mol/L的磷酸二氢钾水溶液为流动相,流速1.0 mL/min;紫外检测器,检测波长195 nm,外标法对草铵膦铵盐的有效成分进行定量分析.结果表明,草铵膦的线性范围为0.254～2.496 g/L,相关系数为0.99914,13.5%草铵膦水剂的标准偏差为0.152,变异系数为1.137%,草铵膦原药的标准偏差为0.2013,变异系数为0.2113%,平均回收率:13.5%草铵膦水剂为100.5%,95%草铵膦原药为99.5%.此方法可用于原药和制剂的分析检测.     

利用消失点信息的建筑物检测

以往的单片建筑物检测,常假设相片是下视或近乎下视成像的,从而可忽略建筑物的透视形变。该文将长度约束与高斯映射和向量叉积相结合,提出了长度优先的向量叉积消失点检测方法,试验表明,该方法对分辨率较高且透视效果明显的图像,有着较好的检测效果。     

Charge-State-Enhanced Ion Sputtering of Metallic Gold Nanoislands

Experimental results on the charge-state-dependent sputtering of metallic gold nanoislands are presented. Irradiations with slow highly charged ions of metallic targets were previously considered to show no charge state dependent effects on ion-induced material modification, since these materials possess enough free electrons to dissipate the deposited potential energy before electron-phonon coupling can set in. By reducing the size of the target material down to the nanometer regime and thus enabling a geometric energy confinement, a possibility is demonstrated to erode metallic surfaces by charge state related effects in contrast to regular kinetic sputtering.