Effect of ethyl alcohol on growth and intracellular alanine racemase of psychrotrophs

The psychrotrophic alanine racemase from Pseudomonas fluorescens, a typical psychrotroph, is less resistant to organic solvents than the enzymes from thermophilic and mesophilic bacteria (Okubo et al., J. Home Econ. Jpn., 46: 1135-1140, 1995). To further elucidate this difference, we examined the effect of ethyl alcohol on the growth and intracellular alanine racemase activity of three typical psychrotrophs-P. fluorescens, Bacillus psychrosaccharolyticus and B. psychrophilus-in comparison with two mesophiles, Escherichia coli and B. subtilis. Although all the bacteria grew to the early stationary phase when cultivated at 22 degrees C for 36 h in the absence of ethyl alcohol, the growth of the psychrotrophs was more effectively suppressed by the addition of 3 and 5% ethyl alcohol to the medium than that of the mesophiles. The intracellular alanine racemase activity of the psychrotrophs was also more markedly reduced in the presence of ethyl alcohol than that of the mesophiles. When bacterial cells of each strain grown at 22 degrees C for 36 h in the absence of alcohol were suspended in 0-5 % ethyl alcohol solution and incubated at 30 degrees C for 1 h, both the survival ratio and intracellular alanine racemase activity of the psychrotrophs were lower than those of the mesophiles. Thus, ethyl alcohol effectively reduced both the growth of the psychrotrophs and their intracellular alanine racemase activity. Low concentrations of various other alcohols also repressed the growth of the psychrotrophs at 10 degrees C.     

Ytterbium-decreasing Streptomyces sp. and its naphthoquinone-pigment production in the presence of rare-earth elements

We screened for oligotrophic microorganisms capable of decreasing the concentration of ytterbium (Yb), a representative of the heavy rare-earth elements, in a culture medium. From 476 strains of oligotrophic microorganisms (grown on 1/100 diluted nutrient agar) isolated from soil and river water samples, 5 strains capable of reducing the concentration of Yb in diluted nutrient broth containing 5 ppm Yb were selected. Among them, a strain capable of reducing the concentration of all rare-earth elements to a great extent was identified as Streptomyces sp. (strain YB-1). This strain produced redish-purple pigment(s) only in the presence of rare earths, but not in that of other metals. The pigment was extracted with ethyl acetate and purified by a series of column chromatography steps. From the results of structural analysis using ultraviolet or infrared absorption spectrometry and 13C-NMR, the pigment was determined to be a kind of naphthoquinone similar to nanaomycin produced by a Streptomyces sp. These results suggested that rare earths might affect the physiological activity of this strain.     

Three-dimensional signal processing for full utilization of NTSC signals and transmission capacities

Three-dimensional (3D) signal processing for TV signals and its applications to higher-picture-quality TV systems are described. Here, “3D” implies “horizontal-vertical-temporal”. The main applications mentioned in this paper are 3D signal processing schemes in ATV (Advanced TV), such as IDTV (ImproveD TV) and EDTV (EnhanceD/Extended Definition TV). In IDTV, proscan conversion is described as the interpolation of 3D sampling, and 3D YC separation as demodulation of 3D modulation. In EDTV, the high-resolution Y component is multiplexed into the “Hole” an inefficiently used space in the 3D frequency domain, by modulating a 3D subcarrier.     

Electron transport through the MOCVD grown GaAs/AlGaAs/GaAs heterojunction barrier

We investigated the current/voltage characteristics of n+GaAs/undoped AlxGa1?xAs (200 A)/n+GaAs heterostructures grown by the metalorganic chemical vapour deposition (MOCVD) method between 77 K and 300 K. Both the voltage dependence and the temperature dependence of the current were in good agreement over a wide current range with the calculated results of the tunnelling and thermionic emission current.     

Outer-loop control of target SIR for fast transmit power control inturbo-coded W-CDMA mobile radio

A technique for obtaining outer-loop control of the target SIR for closed-loop fast transmit power control (TPC) is presented for turbo-coded wideband DS-CDMA (W-CDMA) mobile radio systems. The frame error rate (FER) of an intermediate decoding output in the turbo-decoder. i.e. before the final iteration stage is reached, is measured to control the target SIR. The bit error rate (BER) after the final decoding iteration can be maintained accurately at the prescribed BER value, e.g. BER=10^-6, without losing the tracking ability in the presence of slowly changing propagation channel conditions. Computer simulation results supporting this ability are reported     

Adaptive control of surviving symbol replica candidates in QRM-MLD for OFDM MIMO multiplexing

This paper proposes adaptive control of the number of surviving symbol replica candidates, S/sub m/ (m denotes the stage index), based on the minimum accumulated branch metric of each stage in maximum-likelihood detection employing QR decomposition and the M-algorithm (QRM-MLD) in orthogonal frequency-division multiplexing with multiple-input-multiple-output (MIMO) multiplexing. In the proposed algorithm, S/sub m/ at the mth stage (1/spl les/m/spl les/N/sub t/, N/sub t/ is the number of transmission antenna branches) is independently controlled using the threshold value calculated from the minimum accumulated branch metric at that stage and the estimated noise power. We compared the computational complexity of QRM-MLD employing the proposed algorithm with that of conventional methods at the same average packet error rate assuming the information bit rate of 1.048 Gb/s in a 100-MHz channel bandwidth (i.e., frequency efficiency of approximately 10 bit/s/Hz) using 16QAM modulation and turbo coding with the coding rate of 8/9 in 4-by-4 MIMO multiplexing. Computer simulation results show that the average computational complexity of the branch metrics, i.e., squared Euclidian distances, of the proposed adaptive independent S/sub m/ control method is decreased to approximately 38% that of the conventional adaptive common S/sub m/ control and to approximately 30% that of the fixed S/sub m/ method (S/sub m/=M=16), assuming fair conditions such that the maximum number of surviving symbol replicas at each stage is set to M/spl circ/=16.     

Spatial mapping of electronic states in κ-(BEDT-TTF)2X using infrared reflectivity

We review our recent work on spatial inhomogeneity of the electronic states in the strongly correlated molecular conductors κ-(BEDT-TTF)₂X. Spatial mapping of infrared spectra (SMIS) is used for imaging the distribution of the local electronic states. In molecular materials, the infrared response of the specific molecular vibration mode with a strong electron–molecular vibration coupling can reflect the electronic states via the change in the vibration frequency. By spatially mapping the frequency shift of the molecular vibration mode, an electronic phase separation has been visualized near the first-order Mott transition in the bandwidth-controlled organic conductor κ-(BEDT-TTF)₂Cu[N(CN)₂]Br. In addition to reviewing SMIS of the phase separation, we briefly mention the electronic and optical properties of κ-(BEDT-TTF)₂X.     

Effects of Water and Gelatinized Starch on the Viscoelasticity of Pizza Dough and the Texture of Pizza Crust

The soft texture of the pizza crust rim is generated by baking at a high temperature for a short period in a stone oven. In the case of baking in an electric oven, the pizza dough is baked at a much lower temperature and for a longer period, resulting in a harder texture. To improve the texture of electric oven-baked pizza crust, the effects of water and gelatinized starch on the viscoelasticity of pizza dough and the texture of pizza crust were investigated. Rheological properties (storage modulus, loss modulus, and yield stress) of pizza dough decreased with an increase in water content. When wheat flour in the dough was partially replaced with pre-gelatinized wheat starch, the rheological properties of the dough were maintained even at a high-water content. These results indicate that water-enriched dough can be prepared with gelatinized starch and baked using an electric oven. There was no significant difference in apparent density between the conventional and modified pizza crusts. Water content of the crumb part of the modified crust was significantly higher than that of the conventional crust. Texture analysis revealed that the modified pizza crust showed significantly lower stress at high strain than the conventional crust. In addition, sensory evaluation showed that the modified pizza crust exhibited greater firmness and stickiness than the conventional crust, which was attributed to the increased water content with gelatinized starch of the dough.     

Dual-Function Semaphorin 4D Released by Platelets: Suppression of Osteoblastogenesis and Promotion of Osteoclastogenesis

Effects of the antiosteoblastogenesis factor Semaphorin 4D (Sema4D), expressed by thrombin-activated platelets (TPs), on osteoblastogenesis, as well as osteoclastogenesis, were investigated in vitro. Intact platelets released both Sema4D and IGF-1. However, in response to stimulation with thrombin, platelets upregulated the release of Sema4D, but not IGF-1. Anti-Sema4D-neutralizing monoclonal antibody (mAb) upregulated TP-mediated osteoblastogenesis in MC3T3-E1 osteoblast precursors. MC3T3-E1 cells exposed to TPs induced phosphorylation of Akt and ERK further upregulated by the addition of anti-sema4D-mAb, suggesting the suppressive effects of TP-expressing Sema4D on osteoblastogenesis. On the other hand, TPs promoted RANKL-mediated osteoclastogenesis in the primary culture of bone-marrow-derived mononuclear cells (BMMCs). Among the known three receptors of Sema4D, including Plexin B1, Plexin B2 and CD72, little Plexin B2 was detected, and no Plexin B1 was detected, but a high level of CD72 mRNA was detected in RANKL-stimulated BMMCs by qPCR. Both anti-Sema4D-mAb and anti-CD72-mAb suppressed RANKL-induced osteoclast formation and bone resorptive activity, suggesting that Sema4D released by TPs promotes osteoclastogenesis via ligation to a CD72 receptor. This study demonstrated that Sema4D released by TPs suppresses osteogenic activity and promotes osteoclastogenesis, suggesting the novel property of platelets in bone-remodeling processes.