A genetic algorithm for multiple molecular sequence alignment

MOTIVATION: Multiple molecular sequence alignment is among the most important and most challenging tasks in computational biology. The currently used alignment techniques are characterized by great computational complexity, which prevents their wider use. This research is aimed at developing a new technique for efficient multiple sequence alignment. APPROACH: The new method is based on genetic algorithms. Genetic algorithms are stochastic approaches for efficient and robust searching. By converting biomolecular sequence alignment into a problem of searching for optimal or near-optimal points in an 'alignment space', a genetic algorithm can be used to find good alignments very efficiently. RESULTS: Experiments on real data sets have shown that the average computing time of this technique may be two or three orders lower than that of a technique based on pairwise dynamic programming, while the alignment qualities are very similar. AVAILABILITY: A C program on UNIX has been written to implement the technique. It is available on request from the authors.     

用于非均匀介质重建的选代算法收敛性的研究

研究轴对称二维非均匀介质重建迭代算法的收敛性问题。首先借助于玻恩近似将非线性积分方程线性化，然后，利用玻恩迭代法和变形玻恩迭代法，由散射场数据对轴对称二维非均匀介质进行重建。通过几个例子研究了影响迭代算法收敛性的几种因素，如送代算法本身、积分离散化后的网格划分、正则化方法中的正则化参数选取等。     

新型无灰分散剂的结构分析研究

通过薄膜渗析、红外光谱、凝胶渗透色谱、皂化反应等一系列手段建立了一套对无灰分散剂的系统分析方法，并利用该方法得到了一种新型无灰分散剂及其衍生物的平均结构、相对分子质量分布、分散度等重要结构信息，得出该新型无灰分散剂的结构与传统无灰分散剂的结构不同。     

气流式加速度传感器的敏感机理

介绍了在研制气流式加速度传感器过程中，为对气流式加速度传感器进行建模，作者对气流式加速度传感器的工作机理进行了理论性探讨。在分析过程中以流体力学、传热学和惯性理论为基础并结合实验结果，建立了适合于气流式加速度传感器的气体运动方程和能量方程。同时还讨论了热线测量加速度的方法及根据实验分析了输入加速度对腔体内温度及传感器输出电压的影响，从而为进一步研究一系列气流式传感器的工作机理提供了方法。     

激光加热在Al2O3/Ti系梯度功能材料制备过程中的作用及机理

梯度功能材料是一种界面连续化的新型复合材料,本文研究了激光加热在合成Al2O3/Ti系梯度功能材料过程中的作用和机理,同时用激光大光斑加热方法制备出直径11mm厚度3mm完整的FGM试样并加以分析,以及首次采用在试样中埋设微细热电偶成功地测出了激光加热造成的温度梯度分布。     

用VISUAL FOXPRO设计专业产品数据库

根据专业产品数据库的特点和安全性要求提出了基本设计思想，包括根据用户权限进行检索，建立了Ａ，Ｂ库，形成不同视图以满足不同用户需求。     

水力喷砂割缝增产增注技术的作用原理及展望

本文对从俄罗斯引进的水力喷砂割缝技术的发展过程、工作原理及该技术在大庆油田的试验情况做了详细的阐述。并对它的改进及其将来的推广与发展前景做了展望。     

应用图论及广义简约梯度法进行压力管网优化设计

广义简约梯度法(GRG)是非线性规划算法中最有效的算法之一，本文把图论应用于广义简约梯度法优化的过程中，使输入简便，计算简化，应用GRG算法对武汉市的汉阳管网进行了模拟优化设计，并对管段的计算管径用线性规划法进行标准化，取得了满意的结果     

一种复合制导导弹的导航滤波器设计

研究了一种中制导段采用雷达修正的遥控制导方式和末制导段采用捷联式自寻的导引头的自导引制导方式的捷联式复合制导的中远程战术导弹的导航滤波问题，考虑到目标的大机动性和模型的非线性，基于最佳估计理论中的广义卡尔曼滤波理论，建立了通用导航滤波方程，仿真计算与仿真结果表明所做设计是合理和有效的。     

B7 blockade prevents activation-induced cell death of thymocytes

Although both B7 and its counter-receptor CD28 are expressed in the thymus, the role of B7 in thymic selection is not clear. We investigated the role of B7 in intrathymic deletion of antigen-specific T cells using a TCR transgenic model specific for antigen ovalbumin (OVA) and H-2Ad. Intraperitoneal injection of OVA induced apoptosis of thymocytes and drastic reduction of thymocyte numbers. This was significantly inhibited by co-injection of CTLA-4-Ig which blocks B7 co-stimulation. Deletion of T cells in the thymus following i.p. injection of OVA was associated with T cell pre-activation as demonstrated by T cell proliferation and cytokine production. Injection of CTLA-4-Ig blocked all these activation events and rescued thymocytes from activation-induced cell death. These results demonstrate that B7 is required for the activation-induced cell death of MHC class II-restricted thymocytes in vivo.