The Effect of Mixed-Solvent and Terpenes on Percutaneous Absorption of Meloxicam Gel

The purpose of the present study was to develop the meloxicam transdermal dosage form. The response surface methodology was used to obtain an appropriate mixed-solvent system of pH-7.4 buffer and ethanol for preparing meloxicam hydrogel. The enhancement effects of terpenes on drug precautious absorption were evaluated via in vitro and in vivo study. The result showed that the solubility of meloxicam was dependent on the pH value of buffer solution. The mixed-solvent system of pH-7.4 buffer and ethanol had a synergistic effect on the increase of drug solubility. The highest solubility was obtained in the ratio of 50/50 pH 7.4 buffer/ethanol. A series of terpenes were used as enhancer for improving the penetration rate of meloxicam. The penetration rates were significantly increased by about 70–593 fold and the lag times were shortened from 7.92 to 0.17 hr by enhancer incorporation. Among these terpenes, menthol showed the greatest effect. In vivo penetration study, the AUC_48h was increased by about 1.7 fold by the addition of 5% menthol as enhancer.     

Improved genetic algorithm for mixed-discrete-continuous design optimization problems

An improved genetic algorithm (IGA) is presented to solve the mixed-discrete-continuous design optimization problems. The IGA approach combines the traditional genetic algorithm with the experimental design method. The experimental design method is incorporated in the crossover operations to systematically select better genes to tailor the crossover operations in order to find the representative chromosomes to be the new potential offspring, so that the IGA approach possesses the merit of global exploration and obtains better solutions. The presented IGA approach is effectively applied to solve one structural and five mechanical engineering problems. The computational results show that the presented IGA approach can obtain better solutions than both the GA-based and the particle-swarm-optimizer-based methods reported recently.     

The p38 and NFκB signaling protein activation involved in glycitein protective effects on isoproterenol-treated H9c2 cardiomyoblast cells

Heart disease (HD) is greatly associated with gender and clinical evidence shows that increased serum norepinephrine levels are found in patients with HD. This study investigates the cardio-protective effect of glycitein, a selective estrogen receptor modulator (SERM) from soy bean extract, on H9c2 cardiomyoblast cells treated with isoproterenol (ISO, a norepinephrine analog). The image data and results from western blotting showed that ISO treatment was capable of inducing cellular apoptosis, especially the mitochondrial dependent pathway. Glycitein treatment could suppress mitochondrial pro-apoptotic proteins expression including caspase-9 and caspase-3 in H9c2 treated with ISO. In contrast, several survival proteins were expressed in H9c2 cells treated with glycitein, such as phosphor (p)-Akt, p-Bad and Akt. We confirmed that the protective role of glycitein was partially mediated through the expression of p-38 and NFκB proteins by adding several pathway inhibitors.     

The indirect synthesized method induced relaxor state to ferroelectric characteristics for Pb0.95La0.05(Fe2/3W1/3)0.65Ti0.35O3 ceramics

Pb_0.95La_0.05(Fe_2/3W_1/3)_0.65Ti_0.35O₃ ceramics are synthesized by using the conventional direct oxide synthesized (DS) method and the indirect synthesized (IS) method. The low-field dielectric response is investigated by using the empirical law, two ordering models and the space charge polarization. The temperature-dependent dielectric peak of the samples prepared using the IS synthesized method is obviously increased; the temperature-dielectric constant is changed to sharp and the diffused phase characteristic is decreased. It is concluded that the IS synthesized method can change the dielectric natures from a relaxor like behavior to a sharp ferroelectric state. The space charge polarization is also changed by using the IS synthesized method. According to these results, it is suggested that the 1:1 ordered domain is enhanced based on the random-site type by using the IS synthesized method for Pb_0.95La_0.05(Fe_2/3W_1/3)_0.65Ti_0.35O₃ ceramics. However, if the sintering temperature is not high enough for some calcined powders, the random-site type can be changed to the space charge type.     

Comparison of the pulsed field gel electrophoresis patterns and virulence profiles of the multidrug resistant strains of Salmonella enterica serovar Schwarzengrund isolated from chicken meat and humans in Taiwan

Salmonella Schwarzengrund is one of the frequent serovars isolated from chicken meat in Taiwan. This organism is also one of the invasive Salmonella serovars which may cause human salmonellosis and animal infections. In this study, a total of 466 strains of S. Schwarzengrund including 232 retail chicken meat isolates and 234 human isolates in Taiwan were analyzed for their antibiotic resistance and pulsed field gel electrophoresis (PFGE) patterns. For XbaI-digested DNA, a total of 110 PFGE patterns were obtained. When patterns from both origins were analyzed, of these patterns, 21 were shared by isolates from chicken meat samples and humans. In these 21 patterns, 153 (32.8%) isolates from both origins shared the top five patterns. Since ACSSXTT R-type strains are the major concern worldwide and they accounted for 74.5% of total strains used in this study, such R-type strains in the top five XbaI-digested patterns were then further analyzed with AvrII digestion followed by PFGE and PCR assay targeted to 10 Salmonella virulence genes, i.e., avrA, ssaQ, mgtC, siiD, sopB, gipA, sodC1, sopE1, spvC, and bcfC. When PFGE patterns and virulence gene profiles were combined for the analysis of ACSSXTT R-type strains of S. Schwarzengrund, 29 strains from both origins showed the same pattern combinations. Such results suggested the possible transmission of S. Schwarzengrund from chicken meat to humans.     

Inductive modulation on P-glycoprotein and cytochrome 3A by resveratrol,a constituent of grapes

Resveratrol has been reported to show various beneficial pharmacological effects. Nowadays, resveratrol dietary supplement (RDS) is available in the market. Cyclosporin (CsA), a probe drug of P-glycoprotein (P-gp) and cytochrome P450 3A4 (CYP3A4), is an important immunosuppressant. This study investigated the effect of coadministration of RDS on CsA pharmacokinetics.     

Selective Leaching Process for Neodymium Recovery from Scrap Nd-Fe-B Magnet

Neodymium-iron-boron (Nd-Fe-B) magnets were most widely applied to permanent magnetic products in the world due to their high magnetic force. The increasing growth of scrap Nd-Fe-B magnets resulted in disposal problems and the reduction of neodymium (Nd) valuable resources. In this study, we developed a simple hydrometallurgical precipitation process with pH adjustment to separate and recover Nd 100 pct recovery from scrap Nd-Fe-B magnets. Several physical and chemical methods such as demagnetization, grinding, screening, and leaching processes were also adopted to investigate the recovery of Nd and other metals from scrap Nd-Fe-B magnets. The leaching process was carried out with four leaching reagents such as NaOH, HCl, HNO₃, and H₂SO₄. Batch studies were also conducted to optimize the leaching operating conditions with respect to leaching time, concentration of leaching reagent, temperature, and solid/liquid ratio for both HCl and H₂SO₄ leaching reagents. Nd was successfully separated and recovered with 75.41 wt pct from optimized H₂SO₄ leaching solution through precipitation. Further, the purity and weight percentage of the obtained Nd product was analyzed using scanning electron microscopy–energy-dispersive spectroscopy (SEM-EDS) analysis. An X-ray diffraction (XRD) study confirmed the obtained product of Nd was in the form of NdOOH and Nd(OH)₃.     

Physiographic Drainage-Inundation Model Based Flooding Vulnerability Assessment

Flooding vulnerability assessment is an important issue in Taiwan since Taiwan lies within the most active tropical cyclone formation zone of the Western Pacific. Huge economic damages and losses of human lives are occurred almost every year. This study aims to evaluate flooding vulnerability of a given area subject to large-scale land developments. A scoring-based approach associated with a physiographic drainage-inundation model is developed to quantitatively evaluate vulnerability for flooding. The flooding vulnerability index defined as the product of an exposure score and a hazard score. The exposure score assesses relative losses exposed to flooding, which is determined by land-uses classification. The hazard score measures flooding severity, which is simultaneously determined by inundation depth and duration that are obtained from the inundation model for a design storm. The Yenshui River basin located in southwestern Taiwan is used an example to illustrate the proposed method. The results show that the projected urbanization plan within the Yenshui River basin would increase flooding vulnerability from 0.371 to 0.472. However, this value is reduced to 0.388 when the mitigation measure has been implemented. The obtained spatial distribution of flooding vulnerability for a design storm provides decision-makers useful information to identify hotspots of the study area and evaluate effects of flood-mitigation measure on flooding risk-reduction.