Reduced epicardial coronary vasodilator capacity in patients with left ventricular hypertrophy

BACKGROUND: Enlargement of the epicardial coronary arteries occurs in left ventricular (LV) hypertrophy as an adaptation to the increased coronary blood flow. METHODS AND RESULTS: Vasodilator capacity of the epicardial coronary arteries was determined in 44 patients. The dose-response relation of intracoronary nitroglycerin was assessed in 14 patients (7 control subjects and 7 patients with aortic stenosis [study A]) using quantitative coronary angiography. In a second study (B), vasodilator capacity of the epicardial coronary arteries was determined in 15 control subjects and 15 patients with valvular heart disease. In study A, a curvilinear dose-response relation with maximal vasodilation after 90 micrograms intracoronary nitroglycerin was found in both control subjects and patients with aortic stenosis. Vasodilator capacity was reduced in those with aortic stenosis, although sensitivity to nitroglycerin was similar in both groups. In study B, coronary circumferential length at baseline was larger in those with LV hypertrophy (12.2 +/- 2.2 mm) than in control subjects (8.6 +/- 1.5 mm; P < .001); after 100 micrograms intracoronary nitroglycerin, it increased to 12.9 +/- 2.2 mm (6 +/- 5%) in those with LV hypertrophy and to 10.3 +/- 1.5 mm (21 +/- 8%; P < .001) in control subjects. An inverse relation between baseline circumferential length and its percent increase after nitroglycerin was found (r = -.71, P < .001). CONCLUSIONS: Vasodilator capacity of the epicardial coronary arteries is reduced in patients with LV hypertrophy, although sensitivity to nitroglycerin is normal. This may be due to a flow-mediated decrease in coronary vasomotor tone and/or the occurrence of vascular remodeling with an enlargement of the coronary arteries.     

Identification and Quantitation of Allelochemicals from the Lichen Lethariella canariensis: Phytotoxicity and Antioxidative Activity

Phytotoxicity-based extraction and fractionation were employed to separate allelochemicals contained in an extract of Lethariella canariensis. Twelve phenolic substances were isolated from the phytotoxic fraction Letharal of the thalli. These were identified by spectroscopic methods, physicochemical constants, and HPLC chemical correlation, and determined to be atranol (2), chloroatranol (3), hematommic acid (4), chlorohematommic acid (5), methyl hematommate (6), methyl chlorohematommate (7) (new compound), ethyl hematommate (8), ethyl chlorohematommate (9), methyl -orsellinate (10), atranorin (11), chloroatranorin (12), and (+)-usnic acid (13). Further identification and quantification of these allelochemicals in the environment were conducted by HPLC. Several phenolic compounds showed moderate antimicrobial activity. The cytostatic activity of the polyphenols was investigated on U937 and HL-60 cells. All compounds were assayed, with the exception of 10. The Letharal mixture decreased cell viability in both cell lines. Protection against lipid peroxidation was investigated using brain homogenates. Compounds 2, 3, 6, 8, 11, and Letharal decreased H₂O₂/Fe⁺² induced lipid peroxidation in a concentration-dependent manner, while 10 and 13 were unable to protect tissue against oxidative stress.     

A self contained Linux based data acquisition system for 2D detectors with delay line readout

This article describes a fast and self-contained data acquisition system for 2D gas-filled detectors with delay line readout. It allows the realization of time resolved experiments in the millisecond scale. The acquisition system comprises of an industrial PC running Linux, a commercial time-to-digital converter and an in-house developed histogramming PCI card. The PC provides a mass storage for images and a graphical user interface for system monitoring and control. The histogramming card builds images with a maximum count rate of 5 MHz limited by the time-to-digital converter. Histograms are transferred to the PC at 85 MB/s. This card also includes a time frame generator, a calibration channel unit and eight digital outputs for experiment control. The control software was developed for easy integration into a beamline, including scans. The system is fully operational at the Spanish beamline BM16 at the ESRF in France, the neutron beamlines Adam and Eva at the ILL in France, the Max Plank Institute in Stuttgart in Germany, the University of Copenhagen in Denmark and at the future ALBA synchrotron in Spain. Some representative collected images from synchrotron and neutron beamlines are presented.     

The use of remote-sensing techniques to monitor dense reservoir networks in the Brazilian semiarid region

Reservoirs are the main water source in the Brazilian semiarid region, especially in the crystalline-geology watersheds, forming high-density reservoir networks (HdRN). However, in most cases, the construction of these reservoirs has been done without technical supervision. The objective of this work was to map and evaluate the spatial distribution of the 25,000 km² Orós Reservoir Basin (ORB) HdRN, in semiarid Brazil, with the help of remote-sensing tools associated with geographic information systems (GIS). Using LANDSAT 5 images of the end of the 2011 rainy season of the ORB, the remote-sensing technique allowed the identification of 6002 polygons, which corresponded to only 4717 reservoirs (implying a misidentification of 21%). Between 2002 and 2011, a 17.5% increase (and 1.81% annual increase) in the number of reservoirs in the basin was observed, still lower than the annual increment from 1970 to 2002, when an average increase of 2.64% per year was observed, in other studies. The perimeter of the reservoirs ranged from 0.250 to 560 km and the individual water surface area ranged from 0.004 to 195.0 km², resulting in a total surface of 465.0 km². Analysing the surface area of the strategic reservoirs, results showed that the estimation of the surface area (from remote sensing with manual polygon adjustment) yielded values very close to those of the on-site monitored areas, with R² = 0.99 and normalized difference index ranging from ?0.02 to +0.09. The reservoir density in the ORB in 2011 was 0.19 reservoirs km^–2, higher than the recommended optimum density of 0.15 reservoirs km^–2 basin. Analysis of reservoir density by municipality recorded values ranging from 0.02 to 0.40 reservoirs km^–2. The sedimentary-geology municipalities presented a reservoir density on average 80% lower than the that of the crystalline-geology municipalities, indicating a strong relationship between geology and reservoir density. Neither population density nor rainfall explained the spatial distribution of reservoirs within the basin, both yielding R² lower than 0.1. This remote-sensing survey of reservoirs demonstrated two major flaws: the misidentification of shadows as reservoirs and the inability to identify the presence of macrophytes, which negatively affected the number and surface area of the target reservoirs. Despite these problems, remote sensing has been shown to be a technique of great potential in the planning and management of water resources in regions with dense reservoir networks.     

Thermal evolution in air and argon of nanocrystalline MoS2 synthesized under hydrothermal conditions

Nanocrystalline molybdenum sulfide was synthesized between 150°C and 225°C under hydrothermal conditions starting from ammonium heptamolybdate and thiourea. Samples were characterized with X-ray powder diffraction, electron microscopy, nitrogen adsorption, thermoanalysis and infrared spectroscopy. The hydroxyls involved in the synthesis and adsorbed on crystals surface hindered crystallization and samples still recrystallized after the final dehydroxylation step above 300°C, just when hydroxyls were isolated from each other. This also promoted sulfur bond breaking that gave rise to partial transformation of the MoS₂ into MoO₂ when the annealing atmosphere was argon, and to the total transformation of the sulfide into MoO₃ when it was air. The initial MoS₂ crystals were bend; many of them were isolated, and others associated in bundles that formed worm-like grains interacting with each other to produce spherical grains aggregated in clusters. This morphology gave rise to samples with a low specific surface area.     

Differential immediate early gene expression in conditional hepatitis B virus pX-transforming versus nontransforming hepatocyte cell lines

We report construction and characterization of tetracycline-controlled hepatitis B virus pX-expressing hepatocyte (AML12) cell lines. These cell lines were constructed in AML12 clonal isolates (clones 3 and 4), which express constitutively the tetracycline-controlled transactivator. Since pX is implicated in HCC, this immortalized hepatocyte model system was used to investigate the mechanism of pX in transformation. Clonal isolates of 3pX and 4pX lineages display conditional synthesis of pX mRNA and protein and a 2-fold increase in growth saturation density following tetracycline removal, implicating pX in monolayer overgrowth. Interestingly, only 3pX clones display pX-dependent anchorage independence. Clone 3 lineages express hepatocyte nuclear factor-1alpha and hepatocyte-specific marker genes; clone 4 lineages express hepatocyte nuclear factor-1beta and reduced levels of hepatocyte-specific marker genes, suggesting the importance of the differentiated hepatocyte in pX-mediated oncogenic transformation. Importantly, 3pX and 4pX lineages display differential expression of immediate early genes c-fos and ATF3. The pX-transforming 3pX lineage displays early, pX-dependent induction of ATF3 and prolonged induction of c-fos. The nontransforming 4pX cells display an absence of pX-dependent ATF3 induction and transient induction of c-fos. Our results support the direct link of pX expression to oncogenic transformation in 3pX lineage clones and underscore the advantage of this conditional cellular model system for studying mechanisms of pX-mediated oncogenesis.     

Maneuver prediction for road vehicles based on a novel neuro-fuzzy dynamic architecture

Collision avoidance systems for road vehicles may benefit from timely predictions of vehicle maneuvers. This article presents a novel approach for the prediction of maneuvers that copes with noisy measurements and is based on a supervised version of a dynamic FasArt method (SdFasArt). Additionally, the use of size-dependent scatter matrices to compute the activation of the neurons makes the algorithm more adaptable to different data distributions. The results obtained in real tests confirm the goodness of the method.     

A Path-Based Algorithm for the Cross-Nested Logit Stochastic User Equilibrium Traffic Assignment

Abstract:   This article investigates the single-class static stochastic user equilibrium (SUE) problem with separable and additive link costs. A SUE assignment based on the Cross-Nested Logit (CNL) route choice model is presented. The CNL model can better represent route choice behavior compared to the Multinomial Logit (MNL) model, while keeping a closed form equation. The article uses a specific optimization formulation developed for the CNL model, and develops a path-based algorithm for the solution of the CNL-SUE problem based on adaptation of the disaggregate simplicial decomposition (DSD) method. The article illustrates the algorithmic implementation on a real size network and discusses the trade-offs between MNL-SUE and CNL-SUE assignment .