Early mammary gland metabolic and immune responses during natural-like and forceful drying-off in high-yielding dairy cows

The present work compared metabolic and immune responses in genetically high-producing cows that produced a low amount of milk before expected involution and in cows with the same genetic potential that produced copious amounts of milk before their scheduled drying-off. Ten multiparous lactating Israeli Holstein cows producing approximately 10,500 L in the current lactation, without bacterial infection and scheduled for drying-off approximately 60 d before their expected parturition, were studied. Five of the cows that exhibited a sharp, spontaneous reduction in milk yield at the end of their lactation and produced less than ~14 L/d were defined as cows approaching natural involution (ANI), and 5 cows that produced between 25 and 35 L/d were defined as cows approaching forced involution (AFI). Three days before scheduled drying-off, milking was stopped and milk samples were collected from each quarter. After milking cessation, only modest swelling was observed in the udders of the ANI cows. In the ANI cows, lactose and fat concentrations decreased and the fat:lactose concentration ratio indicated that on d 1 and 2 fat concentrations decreased faster than lactose concentration, whereas on d 3, the rate of reduction was about the same for lactose and fat. In contrast, in AFI cows, fat concentrations increased on d 1 and the fat:lactose ratio indicated that changes in fat secretion were minor compared with those of lactose secretion. Rennet clotting time of milk after drying-off in the ANI cows increased, whereas curd firmness decreased rapidly, such that mammary secretions did not coagulate on d 3. In the AFI cows, such significant changes were observed only on d 3. The inflammatory response increased in both groups, but at each stage the increase was greater in ANI cows than in AFI cows. On d 1, the increase in leukocyte numbers in the ANI cows was made up of mononuclear cells (i.e., T lymphocytes and macrophages). In contrast, in the AFI cows, we observed a marked increase in leukocyte numbers, mainly in the form of polymorphonuclear cells. Our data indicate that the abrupt mammary involution induced in AFI cows provoked signs of distress, which were associated with neutrophilia in milk. In contrast, in the ANI cows, cessation of milking occurred without evidence of engorgement of the udder. Physiological differences in ANI and AFI cows are distinct and are reflected in the differences in the leukocyte populations in milk.     

Selected chapters of David Hasson's contribution to water treatment and membrane technology — A tribute from his students

Upon publication of an honorary Desalination issue for Prof. David Hasson, a group of eleven former students, judged this as a good occasion to acknowledge his contribution to scientific aspects of the water treatment industry and membrane technology. Based on his focus on equilibria, deposition and inhibition of scaling species in heat exchangers and reverse osmosis as well as in remineralization applications, this publication provides highlights of how his contributions have promoted innovation in the water industry. The tribute also highlights his influence as an educator on the authors who conducted studies, in these three areas under his coaching and supervision at the Technion–Israel Institute of Technology over the past 50 years.     

Dynamics,Spectra, and Relaxation Phenomena of Excess Electrons in Clusters

In this paper, we review quantum simulation methods for studies of coupled quantum-classical systems and their applications in investigations of dynamics, spectra, and relaxation phenomena of excess electrons in polar molecular and ionic clusters.     

Number of cycles distribution in circulating systems

The characterization of certain circulating systems in terms of the number of cycles distribution (NCD) of the fluid during operation is considered. The theory of stochastic processes is used to obtain the NCD from the cycle time distribution (CTD) for the system. The case of the gamma distributed CTD is solved explicitly and the limiting distribution of the NCD as the operating time becomes large is considered. It is shown that for long operating times, the NCD converges to a normal distribution whose mean and variance depend only on the mean and the variance of the CTD.     

Aetiology of intramammary infection and its effect on milk composition in goat flocks

Although there has been little study of the origin of intramammary infection (IMI) in goats, a common view is that most bacterial infection in goats occurs during milking. In the present study, the dynamics of occurrence of udder infection during and between lactations in three Anglo-Nubian goat farms in Israel was monitored. Coagulase-negative staphylococci were the predominant bacteria in the IMIs. We found that about 15% of the yearling does were already infected with bacteria when they joined the flock, whereas about 8% of the goats that dried-off returned with new IMIs. Moreover, virtually none of the goats acquired infection during lactation. Thus, our study showed that the aetiology of IMI in goats is very similar to that in dairy cows. A preventive treatment during the dry period should, therefore, be considered as an effective means of reducing the current rate of bacterial infections in goats.     

Structures,solvation forces and shear of molecular films in a rough nano-confinement

Investigations of surface roughness effects on the structure, dynamics and rheology of a molecular fluid (hexadecane) confined between solid (gold) surfaces, through the use of large-scale molecular dynamics simulations, reveal a remarkable sensitivity to the confining surface morphology. A most significant reduction of the ordering propensity is found in films confined by stationary rough surfaces with a consequent strong suppression of solvation forces and the development of liquid-like dynamic and response characteristics. When the rough-surface boundaries are set in motion at a high shear rate, the interfacial layers of the film stick to the adjacent solid boundaries, resulting in partial slip inside the film with the development of shear stress in the viscous molecular fluid, unlike the case of atomically flat crystalline boundaries where slip of the confined film at the boundaries is accompanied by vanishingly small shear stress in the film. These results are discussed in the context of the effect of roughness on the boundary conditions used in modeling fluid flow past surfaces, and they suggest that morphological patterning of surfaces could provide ways for controlled modifications of frictional processes in thin-film lubricated nanotribological systems.     

The accelerated centroid decomposition technique for optimal parallel tree evaluation in logarithmic time

A new general parallel algorithmic technique for computations on trees is presented. In particular, it provides the firstn/logn processor,O(logn)-time deterministic EREW PRAM algorithm for expression tree evaluation. The technique solves many other tree problems within the same complexity bounds.     

Modeling and Simulation of Mono-Layer Coating

Coating simulations of nano-suspended particles are implemented using the DEM-CFD method. A new model of capillary interactions, accounting for the microscale interface deformation implicitly but allowing a rather fast calculation time, is implemented. The drying of a nano-suspension liquid layer is modeled and implemented to the DEM code, allowing transient examination of mono-layer aggregation. The effect of particle size on the coating process is investigated. The main contrast in particle size was received in terms of the transition periods to the finite structure. It was found that the non-dimensional liquid height is a correlative parameter between the cases. Aggregation appears to start at approximately the same non-dimensional liquid height. Furthermore, a firm final structure was obtained at a similar liquid level, way before the drying is complete, indicating that the liquid level has a significant control on the assembly until this point.     

Pilot-Scale Evaluation of Chemical Cleaning Protocols for Organic and Biologically Fouled Microfiltration Membranes

The Edward C Little Water Recycling Facility (ECLWRF) is the largest high-purity water recycling facility in the United States. Here, microfiltration (MF) membranes play a critical role in treating the secondary effluent and serving as pretreatment to the downstream reverse osmosis systems. New chemical clean-in-place (CIP) formulations were evaluated through pilot-scale tests for their ability to improve the performance restoration for the Phase III continuous MF (CMF) membranes at the ECLWRF. Membrane autopsies found that the primary fouling mechanisms for the CMF membranes were biological and organic in origin. It was also determined that the current CIP protocol provided an incomplete removal of the biological and organic foulants. The cleaning test results found that the current CIP regime for the Phase III system performed better than the four commercially available cleaning solutions evaluated here. However, improved results were obtained when hydrogen peroxide was added to the current CIP regime consisting of caustic soda and the commercially available Memclean C cleaning solution. The effects of the addition of hydrogen peroxide to the standard cleaning procedure shows some promise; however, further research is needed to understand the cleaning mechanisms and long-term effects of using hydrogen peroxide as a cleaning additive.     

The major component of the trail pheromone of the leaf-cutting ant,Atta sexdens rubropilosa forel

The major component of the trail pheromone of the South American leaf-cutting ant,Atta sexdens rubropilosa Forel, is 3-ethyl-2,5-dimethylpyrazine (II). Methyl and ethyl phenylacetate and methyl 4-methylpyrrole-2-carboxylate (I), which is the major component of the trail pheromone ofA. texana (Buckley) andA. cephalotes (L.), were also identified and may be minor components. The pheromone is stored in the poison gland.Atta sexdens sexdens (L.) also responds strongly to the pyrazine, which in large amounts evokes a weak response fromA. texana, A. cephalotes, andAcromyrmex octospinosus (Reich). Foraging workers ofAtta sexdens rubropilosa did not preferentially pick up baits impregnated with the pyrazine. The pyrazine was puffed into the nests ofA. cephalotes, and a particular response called milling was noted.