Photorhabdus luminescens TccC3 Toxin Targets the Dynamic Population of F-Actin and Impairs Cell Cortex Integrity

Due to its essential role in cellular processes, actin is a common target for bacterial toxins. One such toxin, TccC3, is an effector domain of the ABC-toxin produced by entomopathogenic bacteria of Photorhabdus spp. Unlike other actin-targeting toxins, TccC3 uniquely ADP-ribosylates actin at Thr-148, resulting in the formation of actin aggregates and inhibition of phagocytosis. It has been shown that the fully modified F-actin is resistant to depolymerization by cofilin and gelsolin, but their effects on partially modified actin were not explored. We found that only F-actin unprotected by tropomyosin is the physiological TccC3 substrate. Yet, ADP-ribosylated G-actin can be produced upon cofilin-accelerated F-actin depolymerization, which was only mildly inhibited in partially modified actin. The affinity of TccC3-ADP-ribosylated G-actin for profilin and thymosin-β4 was weakened moderately but sufficiently to potentiate spontaneous polymerization in their presence. Interestingly, the Arp2/3-mediated nucleation was also potentiated by T148-ADP-ribosylation. Notably, even partially modified actin showed reduced bundling by plastins and α-actinin. In agreement with the role of these and other tandem calponin-homology domain actin organizers in the assembly of the cortical actin network, TccC3 induced intense membrane blebbing in cultured cells. Overall, our data suggest that TccC3 imposes a complex action on the cytoskeleton by affecting F-actin nucleation, recycling, and interaction with actin-binding proteins involved in the integration of actin filaments with each other and cellular elements.     

Unsourced Multiple Access for 6G Massive Machine Type Communications

Unsourced multiple access(UMA) is a multi-access technology for massive, low-power, uncoordinated, and unsourced Machine Type Communication(MTC) networks. It ensures transmission reliability under the premise of high energy efficiency.Based on the analysis of the 6G MTC key performance indicators(KPIs) and scenario characteristics,this paper summarizes its requirements for radio access networks. Following this, the existing multiple access models are analyzed under these standards to determine U...     

PGL-CIMS的设计与开发

本文介绍了平果铝业公司CIMS的系统结构、网络结构、软件结构、关键技术等一系列技术问题。     

基于自动检焦信号梯度信息的自适应平滑算法研究

近年来随着航空相机技术不断发展,高分辨率相机将成为未来航空相机的发展趋势;但环境的变化会使光学系统的性能发生显著的、随机的变化,因此,欲在恶劣的环境条件下获得最佳分辨率,必须使相机具有很高的检焦精度,然而由于测量元件及电源等的噪声的存在,大大影响了检焦信号精度;基于中值滤波和梯度信息的平滑滤波算法,介绍了一种新的自适应平滑算法,该算法有效地抑制扰动信号,更为真实的突现出峰值信号,从而大大提高了检焦精度;该方法大大减小了随机干扰对检焦结果的影响,提高了检焦精度,从而每次都能获得较为清晰的图像。     

采用遗传算法优化最小二乘支持向量机参数的方法

支持向量机是建立在统计学习理论上的一种学习算法,较好地解决了小样本学习问题.由不同的参数和核函数构造的支持向量机在性能上存在很大差异,而在参数和核函数的选择上目前还没有明确的理论依据.针对支持向量机的参数选择问题,提出了一种采用遗传算法优化最小二乘支持向量机参数的方法.结合LS-SVMlab工具箱,在MATLAB实验平台的仿真实验表明,该方法提高了支持向量机的参数选择效率,得到的参数对测试样本的分类结果是最优的,从而避免了人为设定参数的不足,同时缩短了优化时间.     

射频电容液位计在海上溢油液位检测中的应用

简单介绍了射频电容液位计进行油水双液位检测的基本原理,阐述了海上溢油回收检测装置的基本构造及其工作过程。针对海上特殊应用环境的测量需求,提出分别按油、按水标定的液位检测方案。油水混合状态下,对2组实验测试数据进行误差和线性度的数值计算,通过曲线拟合对比分析,最终确定了海上溢油回收集油箱油位检测的最优化方案—按油标定检测。实验证明,该方案可以满足现场实际测量环境的要求,液位计的测量精度高、线性度好。     

元宝山电厂快控汽门的研究与实施

在分析和介绍元宝山电厂和辽西电网情况的基础上，着重介绍了对元宝山电厂３００ＭＷ机组实施快控汽门的研究、试验工作及主要的技术成果。该厂３００ＭＷ机组快控汽门方式的投入，提高了电网稳定性和经济效益。     

便携式峰值功率分析仪核心设计

介绍了峰值功率分析仪的基本结构、主要模块的设计以及实现,论述了微波信号处理、信号峰值检波、信号采样和数据处理、测频以及频响误差补偿技术与校准、系统软件实现原理.提出了一种便携式峰值功率分析仪的设计方案.按照该方案设计的便携式峰值功率分析仪实现了对射频信号功率的准确快速测量.     

《精细化工工艺》课程教学改革的研究

文章论述了合理组织教材以突出教材的重点,凸显知识体系的规律性;精讲教材内容,使学生抓住精细化工的实质;课堂教学与实验内容相结合以增强学生对精细化工理论和实验的理解;把科研内容引入教学中以激起学生的学习兴趣及对科学知识探求的热情;充分利用外部教学条件,把教学与精细化工生产实际结合起来,以培养学生的实际技能等方面的内容。     

法国电信IPTV的实践及其对中国数字电视发展的借鉴意义

“MaLigne TV”是法国电信IPTV部署的一个实例．通过为用户提供丰富的节目内容和完善的服务，“MaLigne TV”获得了巨大的成功。以此为基础，结合实际环境．讨论了在中国部署IPTV面临的问题和可能的解决思路。