Dual Inhibition of Pyruvate Dehydrogenase Complex and Respiratory Chain Complex Induces Apoptosis by a Mitochondria-Targeted Fluorescent Organic Arsenical in vitro and in vivo

Based on the potential therapeutic value in targeting mitochondria and the fluorophore tracing ability, a fluorescent mitochondria-targeted organic arsenical PDT-PAO-F16 was fabricated, which not only visualized the cellular distribution, but also exerted anti-cancer activity in vitro and in vivo via targeting pyruvate dehydrogenase complex (PDHC) and respiratory chain complexes in mitochondria. In details, PDT-PAO-F16 mainly accumulated into mitochondria within hours and suppressed the activity of PDHC resulting in the inhibition of ATP synthesis and thermogenesis disorder. Moreover, the suppression of respiratory chain complex I and IV accelerated the mitochondrial dysfunction leading to caspase family-dependent apoptosis. In vivo, the acute promyelocytic leukemia was greatly alleviated in the PDT-PAO-F16 treated group in APL mice model. Our results demonstrated the organic arsenical precursor with fluorescence imaging and target-anticancer efficacy is a promising anticancer drug.     

Gradient-based recursive parameter estimation for a periodically nonuniformly sampled-data Hammerstein–Wiener system based on the key-term separation

The identification of the Hammerstein–Wiener (H-W) systems based on the nonuniform input–output dataset remains a challenging problem. This article studies the identification problem of a periodically nonuniformly sampled-data H-W system. In addition, the product terms of the parameters in the H-W system are inevitable. In order to solve the problem, the key-term separation is applied and two algorithms are proposed. One is the key-term-based forgetting factor stochastic gradient (KT-FFSG) algorithm based on the gradient search. The other is the key-term-based hierarchical forgetting factor stochastic gradient (KT-HFFSG) algorithm. Compared with the KT-FFSG algorithm, the KT-HFFSG algorithm gives more accurate estimates. The simulation results indicate that the proposed algorithms are effective.     

Silver-plated polyamide fabrics with high electromagnetic shielding effectiveness performance prepared by in situ reduction of polydopamine and chemical silvering

In this article, the silver-plated polyamide fabrics (SPPAFs) with high electroconductibility and shielding effectiveness were fabricated by using in situ reduction of polydopamine and chemical silvering. The effects of SPPAFs dopamine (C₈H₁₁O₂N) and silver nitrate (AgNO₃) concentration on surface resistivity and electromagnetic interference shielding effectiveness were studied. The results showed that the surface resistivity of SPPAFs can reach a minimum value of 0.06 ± 0.014 Ω cm⁻¹, when C₈H₁₁O₂N concentration is 4 g L⁻¹ and the AgNO₃ concentration is 120 g L⁻¹. The shielding effectiveness of SPPAFs in the wide frequency range of 10–3000 MHz increases with the increase in the concentration of AgNO₃, and increases first and stabilizes afterward with increasing C₈H₁₁O₂N concentration. When the concentration of C₈H₁₁O₂N and AgNO₃ is 3 and 120 g L⁻¹, respectively, mean shielding effectiveness values in the low-, medium-, and high-frequency bands are 71.3, 73.8, and 76.1 dB, respectively. Moreover, the mean shielding effectiveness values is 83.79 dB in the frequency range of 1.2–2.3 GHz. The dominant shielding mechanism of SPPAFs is the reflected electromagnetic waves and the absorption shielding effectiveness is less than 2 dB. The average electromagnetic shielding values of SPPAFs are above 67 dB after 16 weeks of storage, when C₈H₁₁O₂N concentration is 4 g L⁻¹ and the AgNO₃ concentration is 80 and 100 g L⁻¹. The prepared SPPAFs show promising applications in military textiles and smart wearable clothing. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 48227.     

Hydroxyapatite/tricalcium silicate composites cement derived from novel two-step sol-gel process with good biocompatibility and applications as bone cement and potential coating materials

Tricalcium silicate (C₃S) and hydroxyapatite (HAp) composites were fabricated through the sol-gel process. The aim of this research is to improve the biocompatibility of C₃S through HAp addition and study the potential of using this as coating materials. The composites (HAp/C₃S) were characterised by Fourier transform infrared spectrometry, thermal gravity-differential thermal analysis and X-ray diffraction. The working and setting times of cement pastes were tested using Gillmore needle. Mechanical properties were examined by nanoindentation and material testing system. In vitro biocompatibility of the materials were studied by cell attachment and viability of L929 and MG-63 cells. HAp/C₃S as a coating material on gelatin film were measured with the surface roughness and imaged by scanning electron microscope. With the addition of HAp, no undesirable free CaO was detected with the synthesis by the sol-gel preparation. The pH values of HAp added groups were between 7.54 and 8.76, which were much lower than pure C₃S group (pH?=?11.75). For in vitro studies, the presence of HAp could effectively enhance the cell attachment and viability of both L929 and MG-63 cells grown in the extract or directly on the composites. However, the mechanical properties of the composites were impaired as compared to pure C₃S. Lastly, HAp/C₃S cement could be evenly coated on gelatin film. HAp is successfully demonstrated to improve C₃S biocompatibility with this new composites HAp/C₃S. C-75 (75% C₃S and 25% HAp), in particular, has good biocompatibility, relatively high compressive strength and can be uniformly coated onto gelatin film. Thus, C-75 is a promising material for further investigation as a coating on other biopolymers.     

CAN总线的输送控制系统集成化研究

针对现有技术中输送系统控制不稳定、控制能力低下等问题,本文提出基于工业CAN总线的输送系统集成化控制.在控制系统CAN通信节点利用代码进行数据的转化,通过emit关键字将数据绘制成显示曲线,由输入的代码不断对系统数据进行刷新,实现了输送系统的实时监控,提高了系统运行的安全性.通过时间同步技术将SAEAS6802时钟同步协议与时钟修正算法相结合,有效地对数据处理时间和接收时间进行校正,减少了数据接收误差,提高了时间同步精度.实验结果表明,本文研究方法提高了输送控制系统的运行安全性和可靠性.     

Improving the initialization speed for long-range NRTK in network solution mode

Initialization speed is one of the most important factors in network real time kinematic (NRTK) performance. Owing to the low correlation among the error s