Myoglobin/gold nanoparticles/carbon spheres 3-D architecture for the fabrication of a novel biosensor

A novel biosensor based on a myoglobin/gold nanoparticles/carbon spheres (Mb-AuNPs-CNs) 3-D architecture bioconjunction has been fabricated for the determination of hydrogen peroxide (H₂O₂). Cyclic voltammetry (CV), Fourier transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM) were used to characterize the bioconjunction of the AuNPs-CNs with Mb. Experimental results demonstrate that the AuNPs-CNs hybrid material is more effective in facilitating electron transfer of the immobilized enzyme than CNs alone, which can be attributed to the unique nanostructure and larger surface area of the bioconjunction. The biosensor displayed good performance for the detection of H₂O₂ with a wide linear range from 0.28 μmol/L to 116.5 μmol/L and a detection limit of 0.12 μmol/L. The Michaelis-Menten constant K _M^app value was estimated to be 0.3 mmol/L. The resulting biosensor exhibited fast amperometric response, and good stability, reproducibility, and selectivity to H₂O₂. This article is published with open access at Springerlink.com     

Direct growth of graphene on vertically standing glass by a metal-free chemical vapor deposition method

A new method to directly grow graphene on quartz glass substrate by atmospheric-pressure chemical vapor deposition (CVD) without using any catalyst was developed. The prime feature of this method is to build a vertical-glass model in the quartz tube to significantly increase the collision probability of the carbon precursors and reactive fragments between each other with the glass surface. The growth rate of high-quality graphene on glass remarkably increases compared with the conventional gas flow CVD technique. The optical transmittance and sheet resistance of the graphene glass can be readily adjusted by regulating growth time. When growth time is 35?min, the graphene glass presents an intriguing sheet resistance of about 1.48 kΩ sq^?1 at a transmittance of 93.08% and exhibits an excellent hydrophobic performance. The method is simple and scalable, and might stimulate various potential applications of transparent and conductive graphene glass in practical fields.     

醋酸分子印迹电化学传感器的制备

基于循环伏安电化学聚合方法,以30mmol/L醋酸作为模板分子,5mmol/L苯酚作为功能单体,0.1mol/L氯化钾和0.05mol/L磷酸盐缓冲溶液(pH=5.4)作为聚合底液,聚合电位为-0.8~1.2V,扫描圈数为20,以0.1mol/L碳酸氢钠溶液作为洗脱液进行洗脱20min,最终制得性能较佳的醋酸分子印迹电化学传感器。对该传感器进行循环伏安(CV)表征、阻抗表征、扫描电镜的形貌表征、差分脉冲表征,探索了不同pH磷酸盐缓冲溶液作为聚合底液、不同浓度洗脱液和不同洗脱时间对该传感器性能的影响。发现在以0.05mol/L磷酸盐缓冲溶液(pH=5.4)作为最佳底液条件下,醋酸分子印迹电化学传感器与0~45×10^-9 mol/L浓度范围内醋酸分子二者呈现出良好的线性关系,其检出限为2.85×10^-9 mol/L,同时研究了该传感器的重现性和稳定性。     

非电起爆系统可靠性分析与计算

在对影响非电起爆网络可靠度的因素进行认真分析的基础上,运用可靠性理论和统计分析的方法,在前人研究的基础上对各种爆破网络的可靠性进行了计算和探讨。并对各网路可靠性、安全性和经济性进行了比较,提出了较合理的起爆网络系统。     

新型磁性蓄冷材料G—M制冷机的研制

对应用磁性蓄冷材料作为二级蓄冷器填料而研制的大制冷功率两级Ｇ－ Ｗ 制冷机的研究工作进行了总结,给出了理论分析及结构设计。并在一台样机进行了实验研究,通过优化制冷机结构参数和运行参数,使制冷机二级最低制冷温度达７．８ Ｋ、２０ Ｋ 时取得１４．５ Ｗ 的有效制冷量,从理论和试验上对应用磁性蓄冷材料改善Ｇ－ Ｍ 制冷机性能的有效性进行了有益的探索。     

出水方式对水冷芯片散热器换热性能影响

本文采用数值模拟的方法研究了4种不同出流方式的散热器在不同冷却水流量下的换热效果,散热器冷却水进口均为中间喷射式。A、B型散热器均设置一个出口,分别位于散热器的一角及一边的中心;C、D型散热器均设置4个出口,分别位于散热器的四角及四边的中心。对比验证数值模拟结果与实验结果,得到数值模拟相对误差不高于7%。分析散热器的传热系数、速度分布、压力损失、综合系数,结果表明:4出口散热器传热系数不及单出口换热器,但流动阻力较小,散热器综合系数较高,C、D型散热器综合系数较A、B型散热器提高了50%以上,且具有更好的均温性,因此喷射流4出口散热器具有较好的换热和流动效果。     

氧化钇纳米粉体材料的制备

以YCl3为原料 ,以Na2 CO3为添加剂 ,聚乙二醇 4 0 0为分散剂 ,首先制备出晶粒细小的碳酸钇前驱体。在 70 0℃焙烧 1h后制得Y2 O3纳米粉体材料 ,经XRD和TEM检测 ,粒径为 5 0nm ,粒度均匀     

共价有机骨架化合物(COFs)储氢材料研究进展

简要介绍了二维和三维COFs的结构,重点介绍了COFs作为储氢材料的研究现状和提高其储氢性能的改性方法,并对COFs在储氢方面存在的不足和未来的研究方向作出了总结与展望。     

用核糖体工程技术二次开发海洋微生物菌株资源的研究

经对无活性的海洋来源放线菌B3054进行链霉素抗性筛选,获得了一株具有抗肿瘤活性的链霉素抗性突变株SY-1.从该突变株的发酵物中分离得到了4个活性化合物,其中1个为新天然产物.对该菌株突变前后的rpsL基因(编码核糖体蛋白S12)进行分析未发现发生突变,提示突变位点可能在核糖体的另外亚基上.实验结果表明,核糖体工程技术可用于无活性菌株资源的二次开发利用.