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41.
Li Meng Verena Coleman Yu Zhao Mario Ost Anja Voigt Annelies Bunschoten Jaap Keijer Katja Teerds Susanne Klaus 《International journal of molecular sciences》2021,22(7)
In the present investigation, we examined whether a change in whole body energy fluxes could affect ovarian follicular development, employing mice ectopically expressing uncoupling protein 1 in skeletal muscle (UCP1-TG). Female UCP1-TG and wild-type (WT) mice were dissected at the age of 12 weeks. Energy intake and expenditure, activity, body weight and length, and body composition were measured. Plasma insulin, glucose, leptin, plasma fibroblast growth factor 21 (FGF21) and plasma insulin-like growth factor 1 (IGF1) levels were analyzed and ovarian follicle and corpus luteum numbers were counted. IGF1 signaling was analyzed by immunohistochemical staining for the activation of insulin receptor substrate 1/2 (IRS1/2) and AKT. UCP1-TG female mice had increased energy expenditure, reduced body size, maintained adiposity, and decreased IGF1 concentrations compared to their WT littermates, while preantral and antral follicle numbers were reduced by 40% and 60%, respectively. Corpora lutea were absent in 40% of the ovaries of UCP1-TG mice. Phospho-IRS1, phospho-AKT -Ser473 and -Thr308 immunostaining was present in the granulosa cells of antral follicles in WT ovaries, but faint to absent in the antral follicles of UCP1-TG mice. In conclusion, the reduction in circulating IGF1 levels due to the ectopic expression of UCP1 is associated with reduced immunostaining of the IRS1-PI3/AKT pathway, which may negatively affect ovarian follicle development and ovulation. 相似文献
42.
Wouter R. A. Ryckaert Jozef A. L. Ghijselen Jan J. M. Desmet Alex P. M. Van den Bossche Jan A. A. Melkebeek David M. Van de Sype 《Electrical Engineering (Archiv fur Elektrotechnik)》2005,87(6):315-326
Although complete compensation is only possible using true active filters, resistive shunt harmonic impedances (SHI) have
been shown to provide a considerable reduction of the harmonic propagation.
In this paper, the harmonic mitigation potential of a resistive SHI is discussed concerning its influence on the voltage distortion
profile along a typical distribution feeder, with the location of the SHI along the feeder as a parameter. Although the distortion values
are dependent on the SHI location, it is shown that the end of the feeder is generally a good location to install the SHI,
especially when the power system parameters can vary or are unknown.
Calculations are performed on a typical radial distribution feeder. Both the power factor correction capacitors and the non-linear
loads are concentrated in single nodes. The linear loads are disconnected to obtain the worst case for the voltage distortion.
Also some measurements on a scale model of a typical distribution feeder are done and the experimental results confirm the
results obtained from simulations. 相似文献
43.
Quantifying Biomediated Ground Improvement by Ureolysis: Large-Scale Biogrout Experiment 总被引:2,自引:0,他引:2
Leon A. van Paassen Ranajit Ghose Thomas J. M. van der Linden Wouter R. L. van der Star Mark C. M. van Loosdrecht 《Canadian Metallurgical Quarterly》2010,136(12):1721-1728
Biogrouting is a biological ground improvement method, in which microorganisms are used to induce carbonate precipitation in the subsurface in order to increase the strength and stiffness of granular soils. In this paper the results of a large-scale experiment (100?m3) are presented, in which the feasibility of biogrouting as a ground improvement method is investigated using techniques and equipment similar to those used in potential applications. In situ geophysical measurements were used to monitor the biogrouting process during treatment and indicated that the stiffness had increased significantly after one day of treatment. The results of unconfined compressive strength tests on samples which were excavated after treatment were used to assess the distribution of mechanical properties throughout the cemented sand body, which correlated quite well with the results of the in situ geophysical measurements. The stiffness increase could be quantified as a function of the injected volume of grouting agents and the distance from the injection points. These results will serve as an important benchmark for future applications of biomediated ground improvement. 相似文献
44.
Maarten Overdijk Wouter van Diggelen 《International Journal of Computer-Supported Collaborative Learning》2008,3(2):165-192
The use and effects of a CSCL-tool are not always predictable from the properties of the tool alone, but depend on how that
tool is appropriated. This paper presents the findings from a case study about the appropriation of a graphical shared workspace.
When students are presented with a new tool they may encounter competing constraints and multiple possibilities for interacting
with it. We argue that during critical events the students make choices, and in order to collaborate, coordinate these choices
as a group. We study appropriation by looking into the ways in which small groups organize their contributions during a computer-mediated
argumentative discussion. The results of our study illustrate how certain principles for organization emerge from an implicit
negotiation of conventions, with mutual influence between the students and the tool. 相似文献
45.
A biofilm model for flowing systems in the food industry 总被引:1,自引:0,他引:1
den Aantrekker ED Vernooij WW Reij MW Zwietering MH Beumer RR van Schothorst M Boom RM 《Journal of food protection》2003,66(8):1432-1438
When bacteria attach to the walls of pipelines, they can form biofilms, which can cause the recontamination of food products. In order to quantify such recontamination, a one-dimensional biofilm model was developed taking into account adsorption, desorption, and the growth of cells. The model consisted of two mass balances describing increases in biofilm formation at the wall and the accumulation of cells in the liquid phase. The necessary parameters for the model were obtained in laboratory biofilm experiments. These experiments involved a flowing system and the use of Staphylococcus aureus as a model pathogen and silicon tubing as a testing material. S. aureus was inoculated into the system for 2 h, and then the system was changed to a sterile medium. Both biofilm formation and the release of cells into the flowing liquid were measured until steady-state conditions were reached (for up to 9 days). The experiments were performed in duplicate for different flow conditions (i.e., for Reynolds numbers of 3.2, 32, and 170). It was shown that at higher Reynolds numbers, the biofilm developed faster, probably owing to an increase in the transfer of nutrients to the surface. The proposed biofilm model was capable of describing the data obtained for the three different flow conditions with the use of the specific growth rate in the biofilm and the desorption coefficient as fit parameters. The specific growth rates were 0.16, 0.27, and 0.49 h(-1) for Reynolds numbers of 3.2, 32, and 170, respectively, and the desorption coefficients were about 1% of these values. 相似文献
46.
Wouter M.J. Achten Joana Almeida Vincent Fobelets Evelien Bolle Erik Mathijs Virendra P. Singh Dina N. Tewari Louis V. Verchot Bart Muys 《Applied Energy》2010
Since 2003 India has been actively promoting the cultivation of Jatropha on unproductive and degraded lands (wastelands) for the production of biodiesel suitable as transportation fuel. In this paper the life cycle energy balance, global warming potential, acidification potential, eutrophication potential and land use impact on ecosystem quality is evaluated for a small scale, low-input Jatropha biodiesel system established on wasteland in rural India. In addition to the life cycle assessment of the case at hand, the environmental performance of the same system expanded with a biogas installation digesting seed cake was quantified. The environmental impacts were compared to the life cycle impacts of a fossil fuel reference system delivering the same amount of products and functions as the Jatropha biodiesel system under research. The results show that the production and use of Jatropha biodiesel triggers an 82% decrease in non-renewable energy requirement (Net Energy Ratio, NER = 1.85) and a 55% reduction in global warming potential (GWP) compared to the reference fossil-fuel based system. However, there is an increase in acidification (49%) and eutrophication (430%) from the Jatropha system relative to the reference case. Although adding biogas production to the system boosts the energy efficiency of the system (NER = 3.40), the GWP reduction would not increase (51%) due to additional CH4 emissions. For the land use impact, Jatropha improved the structural ecosystem quality when planted on wasteland, but reduced the functional ecosystem quality. Fertilizer application (mainly N) is an important contributor to most negative impact categories. Optimizing fertilization, agronomic practices and genetics are the major system improvement options. 相似文献
47.
Anat Akiva Johanna Melke Sana Ansari Nalan Liv Robin van der Meijden Merijn van Erp Feihu Zhao Merula Stout Wouter H. Nijhuis Cilia de Heus Claudia Muñiz Ortera Job Fermie Judith Klumperman Keita Ito Nico Sommerdijk Sandra Hofmann 《Advanced functional materials》2021,31(17):2010524
Bone formation (osteogenesis) is a complex process in which cellular differentiation and the generation of a mineralized organic matrix are synchronized to produce a hybrid hierarchical architecture. To study the mechanisms of osteogenesis in health and disease, there is a great need for functional model systems that capture in parallel, both cellular and matrix formation processes. Stem cell-based organoids are promising as functional, self-organizing 3D in vitro models for studying the physiology and pathology of various tissues. However, for human bone, no such functional model system is yet available. This study reports the in vitro differentiation of human bone marrow stromal cells into a functional 3D self-organizing co-culture of osteoblasts and osteocytes, creating an organoid for early stage bone (woven bone) formation. It demonstrates the formation of an organoid where osteocytes are embedded within the collagen matrix that is produced by the osteoblasts and mineralized under biological control. Alike in in vivo osteocytes, the embedded osteocytes show network formation and communication via expression of sclerostin. The current system forms the most complete 3D living in vitro model system to investigate osteogenesis, both in physiological and pathological situations, as well as under the influence of external triggers (mechanical stimulation, drug administration). 相似文献
48.
Wim Kellens Wouter Vanneuville Els Verfaillie Ellen Meire Pieter Deckers Philippe De Maeyer 《Water Resources Management》2013,27(10):3585-3606
This paper presents the state of the art of flood risk management in Flanders, a low-lying region in the northern part of Belgium which is vulnerable to flooding. Possible flood hazard sources are not only the many rivers which pass through the Flemish inland, but also the North Sea, which is sensitive to the predicted sea level rise and which can affect large parts of the Flemish coastal area. Due to the expected increase in flood risks in the 21st century, the Flemish government has changed its flood management strategy from a flood control approach to a risk-based approach. Instead of focusing on protection against a certain water level, the objective now is to assure protection against the consequences of a flood, while considering its probability. In the first part, attention is given to the reasoning and functioning of the risk-based approach. Recent improvements to the approach are discussed, as well as the GIS-implementation of the entire model. The functioning of the approach is subsequently demonstrated in two case studies. The second part of the paper discusses future challenges for the flood risk management in Flanders. The driving force behind these challenges is the European Directive on the assessment and management of flood risks, which entered into force in 2007. The Flemish implementation of the directive is discussed and situated in the European landscape. Finally, attention is given to the communication of flood risks to the general public, since the “availability” of flood risk management plans is among the requirements of the EU Floods Directive. 相似文献
49.
Riccioli C Pérez-Marín D Guerrero-Ginel JE Saeys W Garrido-Varo A 《Applied spectroscopy》2011,65(7):771-781
This paper proposes a method based on near-infrared hyperspectral imaging for discriminating between terrestrial and fish species in animal protein by-products used in livestock feed. Four algorithms (Mahalanobis distance, Kennard-Stone, spatial interpolation, and binning) were compared in order to select an appropriate subset of pixels for further partial least squares discriminant analysis (PLS-DA). The method was applied to a set of 50 terrestrial and 40 fish meals analyzed in the 1000-1700 nm range. Models were then tested using an external validation set comprising 45 samples (25 fish and 20 terrestrial). The PLS-DA models obtained using the four subset-selection algorithms yielded a classification accuracy of 99.80%, 99.79%, 99.85%, and 99.61%, respectively. The results represent a first step for the analysis of mixtures of species and suggest that NIR-CI, providing valuable information on the origin of animal components in processed animal proteins, is a promising method that could be used as part of the EU feed control program aimed at eradicating and preventing bovine spongiform encephalopathy (BSE) and related diseases. 相似文献
50.
Wouter A. Serdijn Albert C. Van Der Woerd Jan Davidse Arthur H. M. Van Roermund 《Analog Integrated Circuits and Signal Processing》1995,8(2):131-143
This paper discusses the design of low-voltage low-power fully-integratable automatic gain controls. Four different AGCs are presented, all consisting of three elementary building blocks: a controlled amplifier, a comparator and a voltage follower. Their design is treated separately. As an example, the final section describes an automatic gain control for hearing instruments, realized in a bipolar process. 相似文献