Synthesis and in vitro antimicrobial characterization of Boron-PVA Electrospun nanofibers

Polyvinyl alcohol (PVA) electrospun nanofibers and boric acid (BA) complexes of these nanofibers were synthesized with and without using TritonX-100(TX-100) as a water soluble surfactant (PVA, PVA/BA, PVA/TX-100, PVA/TX-100/BA). The electrospun nanofibers were characterized by SEM, FTIR and TGA. Preliminary results showed that certain amount of boric acid decreased the number of Stapylococcus aureus from 6,8x10⁷(CFU/mL) to 3x10⁷(CFU/mL). In the preparation of polymer, boric acid was used as a crosslinker and antimicrobial agent whereas TX-100 was used as surfactant. The viscosity and conductivity measurements indicated that the BA increased viscosity but decreased conductivity. In contrast, TX-100 increased conductivity without considerable effect on viscosity, which affects the production and resulting nanofiber properties such as diameter and bead formation. The antimicrobial activities of the nanofibers were assayed in vitro by Gram-negative (E. coli) and Gram-positive(S. aureus) bacteria. It was observed that PVA/TX-100/BA showed better antibacterial activity against S.aureus bacteria compared to PVA and PVA/TX-100 nanofibers.     

Economic and environmental impacts of insulation in district heating pipelines

The determination of optimum thickness of insulation is often applied to energy technologies and building projects. In this study, the energy, economic and environmental evaluations of thermal insulation in district heating pipeline are discussed. The optimum insulation thickness, energy saving over a lifetime of 10 years, payback period and emissions of CO₂, CO and SO₂ are calculated for nominal pipe sizes and fuel types based on heating loads in Afyonkarahisar/Turkey. The life cycle cost analysis is used to determine the optimum thickness of the pipeline material in order to take into account the change in inflation that directly affect both the cost of pipeline material and fuels depending on fuel type. The results show that the highest value of optimum insulation thickness, energy savings, emissions and the lowest payback period are reached for a nominal pipe size of 200 mm. About three times more energy saving results by making 200 mm nominal pipe instead of 50 mm. Considering the economical and environmental advantages, the geothermal energy is a better choice and then fuel-oil. When thermal insulation is done in a district heating pipeline, there will be a significant reduction of 21% in the amount of CO₂ emitted to the atmosphere.     

Identification of the building parameters that influence heating and cooling energy loads for apartment buildings in hot-humid climates

Identifying the building parameters that significantly impact energy performance is an important step for enabling the reduction of the heating and cooling energy loads of apartment buildings in the design stage. Implementing passive design techniques for these buildings is not a simple task in most dense cities; their energy performance usually depends on uncertainties in the local climate and many building parameters, such as window size, zone height, and features of materials. For this paper, a sensitivity analysis was performed to determine the most significant parameters for buildings in hot-humid climates by considering the design of an existing apartment building in Izmir, Turkey. The Monte Carlo method is selected for sensitivity and uncertainty analyses with the Latin hypercube sampling (LHC) technique. The results show that the sensitivity of parameters in apartment buildings varies based on the purpose of the energy loads and locations in the building, such as the ground, intermediate, and top floors. In addition, the total window area, the heat transfer coefficient (U) and the solar heat gain coefficient (SHGC) of the glazing based on the orientation have the most considerable influence on the energy performance of apartment buildings in hot-humid climates.     

Comparison of analytical techniques for dynamic trace metal speciation in natural freshwaters

Several techniques for speciation analysis of Cu, Zn, Cd, Pb, and Ni are used in freshwater systems and compared with respect to their performance and to the metal species detected. The analytical techniques comprise the following: (i) diffusion gradients in thin-film gels (DGT); (ii) gel integrated microelectrodes combined to voltammetric in situ profiling system (GIME-VIP); (iii) stripping chronopotentiometry (SCP); (iv) flow-through and hollow fiber permeation liquid membranes (FTPLM and HFPLM); (v) Donnan membrane technique (DMT); (vi) competitive ligand-exchange/stripping voltammetry (CLE-SV). All methods could be used both under hardwater and under softwater conditions, although in some cases problems with detection limits were encountered at the low total concentrations. The detected Cu, Cd, and Pb concentrations decreased in the order DGT > or = GIME-VIP > or = FTPLM > or = HFPLM approximately = DMT (>CLE-SV for Cd), detected Zn decreased as DGT > or = GIME-VIP and Ni as DGT > DMT, in agreement with the known dynamic features of these techniques. Techniques involving in situ measurements (GIME-VIP) or in situ exposure (DGT, DMT, and HFPLM) appear to be appropriate in avoiding artifacts which may occur during sampling and sample handling.     

Perceived susceptibility to and perceived causes of road traffic injuries in an urban and rural area of Tanzania

The aim of the study was to investigate social and behavioral correlates of perceived vulnerability to traffic injuries in an urban and rural setting in Tanzania. In 2002, a sample of 494 adults aged 15 years and above participated in household interviews in Dar es Salaam (urban) and Hai District (rural). The study was part of a population-based survey that collected self-report data on non-fatal injuries. In Dar es Salaam 75 and 82% of males and females, respectively, perceived it as likely that they would experience a traffic injury in general. The corresponding figures in Hai were 63 and 64%. Men rated their road traffic vulnerability similarly to women (OR=0.8, 95% CI 0.5-1.3). Factors associated with high perceived vulnerability as a pedestrian or being injured by a bicycle were amount of road safety information received from health workers and friends, having caused a car to swerve and having crossed a road while talking. Respondents perceived driver recklessness and driver drunkenness as the leading causes of traffic injuries in both areas. Differences were found between the urban and rural setting with respect to perceived risk for traffic injury. The implications of these findings in the context of traffic injury prevention are discussed.     

Phase Portrait Modeling of a Nonlinear System with a Dynamic Fuzzy Network

Fuzzy logic and neural networks are two important technologies for modeling and control of dynamical systems and have been constrained by the non-dynamical nature of their some popular architectures. There exist problems such as large rule bases (i.e., curse of dimensionality), long training times, the need to determine buffer lengths. This article proposes to overcome these major problems in phase portrait modeling of a nonlinear system with a dynamic fuzzy network (DFN) with unconstrained connectivity and with dynamic fuzzy processing units called “feurons”. Nonlinear physical system properties can be encapsulated by DFN. As an example, DFN has been used as the modeler for some nonlinear physical system such as chaotic, limit cycle, oscillator. The minimization of an integral quadratic performance index subject to dynamic equality constraints is considered for a phase portrait modeling application. For gradient computation adjoint sensitivity method has been used. Its computational complexity is significantly less than direct sensitivity method, but it requires a backward integration capability. We used first and approximate second order gradient-based methods including Broyden–Fletcher–Golfarb–Shanno algorithm to update the parameters of the dynamic fuzzy networks yielding faster rate of convergence     

Yield and growth response to potassium of grain sorghum as influenced by variety in a savanna soil of Nigeria

Field trials were conducted at Samaru over a three-year period (1980–82) to study the yield, growth and nutrient concentration of three grain sorghum varieties (L. 187, SK5912 and FFBL) in relation to potassium fertilization in a savanna soil. Potassium application rates were 0, 25, 50 and 75 kg K ha^–1. Year × potassium interactions were not significant although there were significant variety × K interactions. The highest grain yields for var. L.187, SK5912 and FFBL occurred from the application of 25, 50 and 75 kg K ha^–1 respectively. Straw yield was generally increased by K application, which also promoted tillering and hastened flowering in grain sorghum. Although grain weight per head, head number per m², grain number and 1000-grain weight were unaffected by this nutrient, weight per head was reduced by 22.8 per cent. K application enhanced N concentration of sorghum plants but caused decline in P concentration. The highest K rate gave the highest K concentration in each of the three varieties at 7 weeks after planting. Optimum K requirement of grain sorghum would seem to be between 25 and 50 kg K ha^–1.     

Nutrient estimation from an FFQ developed for a black Zimbabwean population

Background  

There is little information in the literature on methods of food composition database development to calculate nutrient intake from food frequency questionnaire (FFQ) data. The aim of this study is to describe the development of an FFQ and a food composition table to calculate nutrient intake in a Black Zimbabwean population.     

Correlation between Schmidt hardness,uniaxial compressive strength and Young’s modulus for andesites,basalts and tuffs

Volcanic rocks from the Bodrum Peninsula have been studied to establish a relationship between the uniaxial compressive strength (UCS), Youngs modulus (E) and Schmidt hammer rebound number (N). Test results obtained on andesites, basalts and tuffs have been assessed statistically and the parameters obtained correlated. Regression equations were established between N, E and UCS.The study suggests the following empirical relationships: UCS=2.75 N–36.83, E=0.17 UCS+0.28 and E=0.47 N–6.25. The relationships are somewhat different from the correlations suggested by previous studies and should therefore be used only for andesites, basalts and tuffs with degrees of weathering and mineralogical structure similar to those used in the present study. The results obtained using solely the Schmidt hammer test for estimating mechanical properties of rocks are less accurate than when a full suite of laboratory tests is carried out but it is hoped that these empirical equations will help geotechnical engineers making practical decisions at a preliminary site investigation stage.     

Oxythiamine and dehydroepiandrosterone inhibit the nonoxidative synthesis of ribose and tumor cell proliferation

This study investigates the significance of the glucose-6-phosphate dehydrogenase (G6PD) catalyzed oxidative and the transketolase (TK) catalyzed nonoxidative pentose cycle (PC) reactions in the tumor proliferation process by characterizing tumor growth patterns and synthesis of the RNA ribose moiety in the presence of respective inhibitors of G6PD and TK. Mass spectra analysis of 13C-labeled carbons revealed that these PC reactions contribute to over 85% of de novo ribose synthesis in RNA from [1,2-(13)C]glucose in cultured Mia pancreatic adenocarcinoma cells, with the fraction synthesized through the TK pathway predominating (85%). Five days of treatment with the TK inhibitor oxythiamine (OT) and the G6PD inhibitor dehydroepiandrosterone-sulfate (0.5 microM each) exerted a 39 and a 23% maximum inhibitory effect on cell proliferation in culture, which was increased to 60% when the two drugs were administered in combination. In vivo testing of 400 mg/kg OT or dehydroepiandrosterone-sulfate in C57BL/6 mice hosting Ehrlich's ascitic tumor cells revealed a 90.4 and a 46% decrease in the final tumor mass after 3 days of treatment. RNA ribose fractional synthesis through the TK reaction using metabolites directly from glycolysis declined by 9.1 and 23.9% after OT or the combined treatment, respectively. Nonoxidative PC reactions play a central regulating role in the carbon-recruiting process toward de novo nucleic acid ribose synthesis and cell proliferation in vitro and in vivo. Therefore, enzymes or substrates regulating the nonoxidative synthesis of ribose could also be the sites to preferentially target tumor cell proliferation by new anticancer drugs.