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991.
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993.
S Sudo J Tanaka K Toku J Desaki S Matsuda T Arai M Sakanaka N Maeda 《Canadian Metallurgical Quarterly》1998,154(2):499-510
Although microglial cells are well known to become activated in the pathological brain, mechanisms underlying the microglial activation are not fully understood. In the present study, with an aim to elucidate whether neurons are involved in the microglial activation, we compared the morphology and the superoxide anion (O2-)-generating activity of rat microglial cells in pure culture with those of cells cocultured with rat primary cortical neurons. Microglial cells in pure culture in serum-free Eagle's minimum essential medium on poly-L-lysine-coated coverslips displayed ramified morphology and suppressed activity of O2- generation. In contrast, microglial cells in neuron-microglia coculture under the same conditions as those for the pure culture displayed ameboid shape and upregulated activity of O2- generation. Electron microscopic observation revealed that microglial cells in coculture were more abundant in Golgi apparatus and secretory granules than those in pure culture and that some of microglial cells in the vicinity of neurites exhibited membrane specialization reminiscent of a junctional apparatus with high electron density between a microglial soma and a neurite. Microglial cells in coculture tended to tie neurites in bundles by extending processes. Medium conditioned by neurons significantly enhanced O2- generation by microglia, but microglial cells in contact with or in close apposition to cocultured neurons were much more intensely activated than those remote from the neurons. Furthermore, the membrane fraction of cortical neurons activated microglial cells, and this effect was abolished by treating the neuronal membrane with trypsin or neuraminidase. In conclusion, neuronal-microglial contact may be necessary to mediate microglial activation. The present findings suggest that the contact of microglia with damaged neurons in the brain is a plausible cause to activate microglia in the neuropathological processes. 相似文献
994.
Murabayashi F. Hotta T. Tanaka S. Yamauchi T. Yamada H. Nakano T. Kobayashi Y. Bandoh T. 《Solid-State Circuits, IEEE Journal of》1994,29(3):298-302
This paper describes 3.3-V BiCMOS circuit techniques for a 120-MHz RISC microprocessor. The processor is implemented in a 0.5-μm BiCMOS technology with 4-metal-layer structure. The chip includes a 240 MFLOPS fully pipelined 64-b floating point datapath, a 240-MIPS integer datapath, and 24 KB cache, and contains 2.8 million transistors. The processor executes up to four operations at 120 MHz and dissipates 17 W. Novel BiCMOS circuits, such as a 0.6-ns single-ended common base sense amplifier, a 0.46-ns 22-b comparator, and a 0.7-ns path logic adder are applied to the processor. The processor with the proposed BiCMOS circuits has a 11%-47% shorter delay time advantage over a CMOS microprocessor 相似文献
995.
Y Tanaka Y Yoshida M Hirano M Morimoto T Kanaseki 《Canadian Metallurgical Quarterly》1993,107(6):522-526
The distribution of substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactive (ir) fibres in the cat's larynx was investigated utilizing immunohistochemistry. Many SP- and CGRP-ir fibres with varicosities were found within and below the epithelium and along the basement membrane of the mucosa of all different regions except in the membranous portion of the vocal fold. In the subepithelium, some SP- and CGRP-ir nerve bundles and nerve fibres were recognized around the vessels and glands. In the mucosa, the pattern of distribution and the density of SR-ir fibres were similar to those of CGRP-ir fibres. These reactive fibres were denser in the supraglottic region than in the subglottic region. In the taste bud-like structures, only SP-ir fibres appeared, whereas in the motor endplates, CGRP-reaction was found exclusively. The present findings suggest that the regional distribution of SP- and CGRP-immunoreactivity might be related with sensory and autonomic innervation in the larynx. 相似文献
996.
Three experiments tested the ability to integrate information about object–object relationships in 2 chimpanzees. In Experiment 1, the subjects were trained to match 1 part of a 2-part object to its other part, match a tool to its assembled object, match a container to its tool, and match a tool to its container. In Experiment 2, the subjects were trained to match a picture of the sample. One subject learned this type of matching task and was then tested on whether she could choose the pictures of related items in Experiment 1. Although the subject was reinforced irrespective of her choices, she chose pictures of items related to the sample when there was no picture of the sample. Experiment 3 showed that the subject was able to match a picture of the item among related items. The results suggest that the subject might integrate information about relationships acquired in Experiment 1 and organize it to make networks of related items. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
997.
G Richard AR Wright S Harris SZ Doyle B Korge C Mazzanti T Tanaka W Harth OW McBride JG Compton 《Canadian Metallurgical Quarterly》1994,103(5):665-668
Darier's disease (DD) is an autosomal dominant genodermatosis characterized by epidermal acantholysis and dyskeratosis. We have performed genetic linkage studies in 10 families with DD (34 affected) by analyzing 14 polymorphic microsatellite markers. Our results confirm recent reports mapping the DD gene to chromosome 12q23-q24.1. Haplotype analysis of recombinant chromosomes in our families, along with previously reported data, narrow the location of the DD gene to a 5 cM interval flanked by the loci D12S354 and D12S84/D12S105. This localization allowed exclusion of two known genes, PLA2A and PAH, as candidate loci for DD. Three other gene loci (PPP1C, PMCH, PMCA1), mapping in 12q21-q24, remain potential candidates. 相似文献
998.
999.
K Koike T Kawabe T Tanaka S Toh T Uchiumi M Wada S Akiyama M Ono M Kuwano 《Canadian Metallurgical Quarterly》1997,57(24):5475-5479
The human cMOAT gene encodes a membrane protein involved in the ATP-dependent transport of hydrophobic compounds. To determine whether cMOAT is associated with drug sensitivity, we transfected an expression vector containing cMOAT antisense cDNA into the HepG2 human hepatic cancer cell line. We observed a reduction in cMOAT protein, as well as an enhanced level of glutathione, in the antisense transfectants. The transfectants displayed an increased sensitivity to cisplatin, vincristine, doxorubicin, and the camptothecin derivatives, (4S)-4,11-diethyl-4-hydroxy-9-[(4-piperidinopiperidino)carbonyl oxy]dione hydrochloride triethydrate and 7-ethyl-10-hydroxycamptothecin, but not to etoposide, 3-[4-amino-2-methyl-5-pyrimidinyl)methyl]-1-(2-chloroethyl)-1-nitrosoure a, 5-fluorouracil, and mitomycin C. Results suggest that cMOAT levels are inversely correlated with those of glutathione, and that cMOAT and its related genes may be involved in the sensitivity of cells to certain anticancer agents. 相似文献
1000.
[Correction Notice: An erratum for this article was reported in Vol 75(1) of Journal of Applied Psychology (see record 2008-10492-001). An error exists in Figure 2 and the accompanying text of the article. The corrected information is included in the erratum.] The problem of assessing fit of structural equation models is reviewed, and two sampling studies are reported that examine the effects of sample size, estimation method, and model misspecification on fit indices. In the first study, the behavior of indices in a known-population confirmatory factor analysis model is considered. In the second study, the same problem in an empirical data set is examined by looking at antecedents and consequences of work motivation. The findings across the two studies suggest that (a) as might be expected, sample size is an important determinant in assessing model fit; (b) estimator-specific, as opposed to estimator-general, fit indices provide more accurate indications of model fit; and (c) the studied fit indices are differentially sensitive to model misspecification. Some recommendations for the use of structural equation model fit indices are given. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献