Development and application of the DPLEX shield method: Results of experiments using shield and segment models and application of the method in tunnel construction

The DPLEX shield method employs a shield having a parallel link-type excavation mechanism to build shield tunnels of various cross-sectional shapes (rectangle, oval, etc.). The segment used with the DPLEX method has a rational cross-sectional shape, that is, a rounded rectangle obtained by putting together curved sections having different radius of curvature. This paper describes the results of experiments conducted using a shield in work and segment models for the development of the DPLEX shield method.     

(Z,Z)-5,27-Tritriacontadiene: Major sex pheromone ofDrosophila pallidosa (Diptera; Drosophilidae)

A crude cuticular extract from both sexes of 3660 fruit flies (Drosophila pallidosa) was subjected to SiO₂ and AgNO₃/SiO₂ column chromatography, accompanied by bioassay for the sex pheromone activity. After three chromatographic steps, the active fraction was obtained. The main component of the active fraction was determined to be (Z,Z)-5,27-tritriacontadiene [(Z,Z)-5,27-C_33:2, on the basis of gas-liquid chromatographic analysis, chemical derivatization and gas chromatography-mass spectrometry. Synthetic (Z,Z)-5,27-C_33:2 at 5 female equivalents (FE) elicited a clear courtship response with a high courtship index amongD. pallidosa males. Therefore it was concluded that (Z,Z)-5,27-C_33:2 was a major sex pheromone component in this species.     

Apolipoprotein E epsilon 4 allele distribution in Wernicke-Korsakoff syndrome with or without global intellectual deficits

Recent genetic studies show that the apolipoprotein E (ApoE) epsilon 4 allele is a risk factor for Alzheimer's disease (AD). Whether this allele is associated with other dementing diseases is the next important question. The information could provide a clue to the pathogenetic role of ApoE. In the present study, patients with Wernicke-Korsakoff syndrome (WKS) of alcoholic etiology were divided into two groups according to the severity of intellectual deficits, i.e., those of "classical" Korsakoff patients with preserved intellectual function other than amnesia and those with global intellectual deficits. Genotyping showed that the frequency of ApoE epsilon 4 allele was significantly higher in the patients with global deficits, suggesting the involvement of this allele in the intellectual decline of WKS. In contrast, distributions of other two markers, alpha 1-antichymotrypsin and presenilin-1, did not differ between the two groups. These results added further support to the notion that the consequence of acute insult to the brain is influenced by the ApoE genotype, and suggested ApoE's role in the development of a certain group of "alcoholic dementia."     

Variants of the 3' region of the cagA gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases

The CagA protein of Helicobacter pylori is an immunogenic antigen of variable size and unknown function that has been associated with increased virulence as well as two mutually exclusive diseases, duodenal ulcer and gastric carcinoma. The 3' region of the cagA gene contains repeated sequences. To determine whether there are structural changes in the 3' region of cagA that predict outcome of H. pylori infection, we examined 155 cagA gene-positive H. pylori isolates from Japanese patients including 50 patients with simple gastritis, 40 with gastric ulcer, 35 with duodenal ulcer, and 30 with gastric cancer. The 3' region of the cagA gene was amplified by PCR followed by sequencing. CagA proteins were detected by immunoblotting using a polyclonal antibody against recombinant CagA. One hundred forty-five strains yielded PCR products of 642 to 651 bp; 10 strains had products of 756 to 813 bp. The sequence of the 3' region of the cagA gene in Japan differs markedly from the primary sequence of cagA genes from Western isolates. Sequence analysis of the PCR products showed four types of primary gene structure (designated types A, B, C, and D) depending on the type and number of repeats. Six of the seven type C strains were found in patients with gastric cancer (P < 0.01 in comparison to noncancer patients). Comparison of type A and type C strains from patients with gastric cancer showed that type C was associated with higher levels of CagA antibody and more severe degrees of atrophy. Differences in cagA genotype may be useful for molecular epidemiology and may provide a marker for differences in virulence among cagA-positive H. pylori strains.     

PadSpace: A new framework for the service federation of web resources

End-users need a simple and interactive tool for service composition development. A PadSpace proposes an extension of a typical Linda-like coordination model (tuplespace) to provide mechanisms for the interoperation among Web applications, Web services and end-users’ local functional resources. First, a PadSpace provides an end-user supporting tool for composing Web applications, Web services, and local visual resources based on the meme media architecture without writing any program codes. It enables end-users to directly manipulate visual components, and to create new composite components for the creation of services that use Web applications, Web services, and local functional resources. Second, a PadSpace provides a spreadsheet-based service-coordination tool for end-users to orchestrate multiple Web applications, Web services, and local functional resources. Finally, we show some new applications of service composition and service orchestration.     

Residential PV system users' perception of profitability,reliability, and failure risk: An empirical survey in a local Japanese municipality

Although previous studies have addressed the reliability of residential PV systems in order to improve the dissemination of the systems among individual users and societies, few have examined users' perception of their own PV systems, which might contain solutions to firmly establish the system into society. First, the present paper examined the extent to which residential PV system users understand specification, reliability, and failure risk of their own systems. Second, causal factors affecting users' satisfaction with PV systems were examined. By analyzing data collected in Kakegawa City, this paper revealed that users did not appropriately understand the basic specifications of their residential PV systems, and in particular, the fact that the systems sometimes failed and therefore needed proper maintenance. Furthermore, a strong causal relationship between users' expectations of financial return from the system and their level of satisfaction was confirmed empirically. These results suggested that excessive focus on profitability and relatively low interest in the systems' reliability and failure risk should be addressed more to avoid problems that could potentially hamper the establishment of this technology into society.     

Label-free electrochemical immunoassay for the detection of human chorionic gonadotropin hormone

Here we report on a new and rapid immunoassay for the label-free voltammetric detection of human chorionic gonadotropin hormone (hCG) in urine. Monitoring the changes in the current signals of antibodies (Abs) before and after the binding of the antigen (Ag) provides the basis for an immunoassay that is simple, rapid, and cost-effective. Since hCG is found at highly elevated levels in pregnant female urine with the range of 30,000-200,000 mIU/mL (approximately 30-200 nM) by 8-10 weeks into pregnancy, its label-free electrochemical detection was achieved by using our method. The coverage of the electrode surface with the Ab and the incubation time with the target Ag were optimized for the detection of hCG. The limit of detection of our method was calculated to be 15 pM (n = 3, approximately 15 mIU/mL) in synthetic hCG samples and 20 pM (n = 3, approximately 20 mIU/mL) in human urine. The electrochemical results for the detection of hCG in the urine samples were in agreement with the results obtained using a reference system, enzyme-linked immunosorbent assay. Further research about the intrinsic electroactivity of Abs and their target molecules would surely provide new and sensitive screening assays, as well as extensive data regarding their interaction mechanisms.     

Possible use of bulk superconductor with tunnel junctions for nuclear radiation spectroscopy

Nuclear radiation detectors with Josephson junctions, as hitherto reported, were composed of thin superconducting strips and had a poor energy resolution. A conceptual study is made on improvement of these detectors, with special consideration of quasiparticle collection. A bulk superconductor is shown to be appropriate as a radiation detector. Superconducting tunnel junctions of two types are proposed for superconducting radiation detectors of excellent energy resolution. These have high barrier penetrability and large area in order to collect all the quasiparticles generated in the superconductor by nuclear radiation.     

Identification of proteins in human substantia nigra

Characterization of the human brain proteome is a critical area of research. While examination of the human cortex has provided some insight, very little is known about the proteome of the human midbrain, which demonstrates substantial loss of dopaminergic neurons in the substantia nigra pars compacta (SNpc) in Parkinson's disease (PD). Therefore, characterization of this region is essential to a better understanding of the pathogenesis of PD. This dataset paper reports two separate studies, where human SNpc was collected from PD and control subjects and 1263 proteins were identified using MALDI-TOF/TOF as well as linear ion trap MS platforms. With gene ontology analysis, the proteins were categorized according to their biological processes, as well as cellular components. These data were also compared with previous proteomic characterization of the human frontal and temporal cortex, and cerebrospinal fluid to establish shared proteins of relevance. The present dataset is the most extensive survey of the human SNpc proteome, to date. Further characterization of the SNpc proteome will significantly facilitate our understanding of the function and expression of proteins involved in PD, as well as provide potential proteins that may be utilized as biomarkers.     

Context-aware spatio-temporal event prediction via convolutional Hawkes processes

Machine Learning - Massive spatio-temporal event data sets are now available that cover events such as disease outbreaks, armed conflicts and crimes. Predicting such events and revealing the...