Expression of green fluoscrescent protein gene in Sclerotinia sclerotiorum

Protoplasts of the pathogenic plant fungus,Sclerotinia sclerotiorum,were transformed using the pPGF plasmid,which contains green fluorescent protein gene,under the control of Aspergillus nidulans regulatory sequences. The pPGF plasmid was introduced by PEG/CaCl2 treatment. Positive transformants were harvested with hygromycin B (HYG) resistance as selective marker,and then were observed with green fluorescence phenomena in response to blue light,which suggested that GFP gene was cloned into genome DNA of S. sclerotiorum. The transformants were verified mitotically stable by Southern blotting analysis and passage culturing. This study is developed as an initial step for further research into infection mechanisms of S. sclerotiorum to plants and interactions with bio-control fungus.     

Effects of main factors on remedying northern landscaping water by hybrid ecological filter

Natural zeolite and coal cinder were layered as main packing medium of the ecological filter instead of traditional filling to remedy the static lake water in Northern China.The ecological filter was running in a combined upward and downward flow mode.Dynamic experiments were carried out to study the effects of retention time and ambient temperature on pollutants’ removal efficiency of the hybrid ecological filter.The function of plant was also studied by contrast test.It is showed that the removal efficiencies of NH4+-N and TP are increased when the retention time is changed from 1 h to 2 h and 4 h,but the removal efficiency of TN is decreased,the removal efficiency of NH4+-N is increased from 91.5% to 98% and that of TP is increased from 31.8% to 52.5%.When the temperature declines,the temporal removal efficiency of NH4+-N is reduced,but the removal efficiency of 24 h and 48 h is remained.The removal efficiency of TP after 24 h and 48 h is decreased when the temperature declines evidently.The retention time plays an important role in NH4+-N and TP removal,and the ambient temperature is significant for TP removal.The plant favors for TP and organic matter removal but has little effect on TN removal.     

基于高速摄像机的电磁电器动态特性测试及其图像处理的研究

为了研究电磁电器动态特性测试的新方法,以智能交流接触器为例拍摄了智能交流接触器的动态过程,对采集的接触器触头、铁心等动态位移图像进行处理,然后应用二进小波多层多尺度分解完成降噪处理,此外本文采用样条求导方式对运动速度进行软测量,从而获得智能交流接触器的真实动态特性,介绍了电磁电器动态特性测试装置与数据处理新方法。该研究采用非接触测试方式,进行图像拍摄与降噪处理,实现了电磁电器动态过程全面、高速与准确的测量,为加快电磁电器产品的研究开发奠定基础。     

Zonal disintegration phenomenon in enclosing rock mass surrounding deep tunnels -- Elasto-plastic analysis of stress field of enclosing rock mass

The zonal disintegration phenomenon (ZDP) is a typical phenomenon in deep block rock masses. In order to investigate the mechanism of ZDP, an improved non-linear Hock-Brown strength criterion and a bi-linear constitutive model of rock mass were used to analyze the elasto-plastic stress field of the enclosing rock mass around a deep round tunnel. The radius of the plastic region and stress of the enclosing rock mass were obtained by introducing dimensionless parameters of radial distance. The results show that tunneling in deep rock mass causes a maximum stress zone to appear in the vicinity of the boundary of the elastic and the plas-tic zone in the surrounding rock mass. Under the compression of a large tangential force and a small radial force, the rock mass in the maximum stress zone was in an approximate uniaxial loading state, which could lead to a split failure in the rock mass.     

Relationship of hydrocarbon and source-rock in Nos.3-5 tectonic belts of the Lenghu area, northern Qaidam Basin

Based on analyses of the components of crude oil hydrocarbons and carbon isotopes, the content of normal alkane de-creased from 49.00% to 20.10% when moving from the No.3 to No.5 tectonic belt of the Lenghu area of the Qaidam Basin, while cycloalkanes increased from 30.00% to 52.20% and aromatic and branch chain alkanes increased gradually as well. The maturity of sterane in crude oil is higher than that of its source-rock, which shows that the hydrocarbons were generated from a deep source-rock of high maturity around the tectonic belts of the Lenghu area. The analysis of the characteristics of carbon isotopes also shows that these isotopes of hydrocarbon compounds in the No.4 and No.5 tectonic belt are apparently heavier than those in the No.3 belt. The results of our research show that the hydrocarbons in the No.3 tectonic belt are mainly from a relatively rich sapropelic substance, while the hydrocarbons in the No.4 and No.5 tectonic belt originated mainly from organic matter of a rela-tively rich humic type substance.     

Relation between structural evolution of the Longmenshan orogenic zone and sedimentation of its foreland basin

In order to determine the area for oil and gas exploration in China's north Sichuan basin, we have divided the time during which the Longmenshan foreland basin was formed into five periods, based on the sedimentary response relationship of the fore-land basin to structural evolution: 1) a late Triassic Noric period; 2) an early-Middle Jurassic period; 3) a late Jurassic to early Cre-taceous period; 4) a late Cretaceous to Paleogene-Neogene period and 5) the Quaternary period. As well, we analyzed the sedimen-tary environment and lithologic features of every basin-forming period. The results show that there are several favorable source-reservoir-cap assemblages in our study area, making it a major region for future oil and gas exploration in China's northern Sichuan basin.     

考虑初始弯曲矩形薄壁钢管砼柱管壁屈曲分析

实际工程中的钢管都是有初始弯曲的,初始弯曲的存在会降低薄壁钢管混凝土柱的承载力.采用能量法对具有初始弯曲管壁的屈曲性能进行了分析,分析结果表明,初始弯曲的存在会使管壁的宽厚比降低30%左右.     

基于无线网的农业虚拟现实技术的研究、

针对传统农业在作物生长过程中遇到技术问题只能在网上或书上查得文字解决方案的弊端,作者将无线网技术与农业虚拟现实技术相结合,采用虎拟现实技术设计了一套虚拟农业专家系统,该系统使用802．11g作为无线网传输标准．利用作物现场的终端设备对作物生长情况进行实进监测和分析,并及时调用专家系统中的虚拟解决方案进行针对性的处理,从而可将损失减到最小．     

基于模糊层次分析法的应用性技术成果评价

针对现有应用性技术成果的评价指标体系,建立了相应的模糊层次分析法数学模型,此模型可克服传统层次分析法判断矩阵构造主观性强和一致性不易检验等缺点,为应用性技术成果的等级评价提供了一种简便合理的科学评价方法.     

简支梁多位置损伤识别研究

曲率模态是结构损伤识别的敏感标示量,采用数值仿真方法,把曲率模态用于桥梁损伤识别.利用简支梁损伤前后一阶曲率变化率作为识别参数,便可明显确定其多处损伤位置.对相关样本参数进行归一化处理并引入噪声干扰,利用BP人工神经网络进行数值仿真计算,也能对其损伤程度进行有效模拟,验证了BP神经网络也有很强的泛化能力及容错能力.