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51.
52.
We studied the molecular basis of protein C deficiency in a family with a history of thromboembolic disease. An approximately 50% reduction in anticoagulant activity despite normal levels of protein C amidolytic activity and antigen was detected in plasma from the proband. All the exons and intron/exon junctions of the protein C gene were studied using a strategy that combined polymerase chain reaction amplification with DNA sequencing of the amplified fragments. We identified a C-to-A change at nucleotide number 1387 of the protein C gene in the proband and his mother, and this mutant was designated protein C Osaka 10. The C-to-A change resulted in the substitution of Ser for Arg at position -1, which is the processing protease cleavage site. The mutant protein C was partially purified from plasma of the patient's mother using barium adsorption followed by ion-exchange column chromatography. It eluted at the same sodium chloride concentration as normal protein C, and thus gamma-carboxylation of the mutant protein appeared to be normal. The apparent molecular weight of this mutant protein C was the same as that of the normal protein on immunoblotting. Amino-terminal sequence analysis showed that the light chain of the mutant protein C had an additional Ser at position-1. Thus, the loss of anticoagulant activity of protein C Osaka 10 can be explained by alteration of the conformation of the Gla domain by the additional Ser in the mutant molecule. 相似文献
53.
Carboxymethyl-2-diethylaminoethylcellulose (CM-DEAE cellulose) was prepared by etherification of carboxymethylcellulose with diethylaminoethyl chloride in a NaOH solution. The behaviour of CM-DEAE cellulose in aqueous solution was studied by viscosity and GPC measurements. The degree of substitution (DS) and existing states of DEAE substituents were examined by the use of proton NMR, and those of the CM substituents were observed with FT-IR. The results reveal that the reduced viscosity and apparent molecular size of CM-DEAE cellulose vary with the concentration of sodium chloride and changes in pH and DS. These phenomena can be explained in terms of inter- and intra-molecular ionic interactions. 相似文献
54.
The crystallization behaviour of three amorphous alloys, Co50Ni25Si15B10, Ni50Co25Si15B10 and Ni50Co25P15B10, was studied by means of differential thermal analysis in conjunction with scanning transmission electron microscopy. Isochronal annealing showed a strong dependence of crystallization on scan rate over the range of 1.99 to 20.70 K min–1. At high Co/Ni ratios, a sequential two-stage crystallization process involving primary MS-I phase followed by MS-II phase precipitation was observed. At low Co/Ni ratios MS-I and MS-II crystallization were concurrent and inseparable. Replacement of the metalloid Si with P as the glass-former dramatically reduced the activation energy for crystallization as well as the crystallization temperature. A mechanistic understanding of these findings was pursued in light of TEM/STEM microanalysis 相似文献
55.
56.
有扰对称信道平均信息量的计算 总被引:3,自引:1,他引:2
照相手机让诺基亚抢了风头,这真是亚洲人的失策。在日本和韩国,内置摄像头的手机早就不是什么值得炫耀的东西了。不过日本人的动作还是很快的,GD88和N8一副后来居上的架势,让人不敢小瞧。 相似文献
57.
10000m3/h制氧机板式换热器损坏修复后,热端跑冷严重,氧气取出量不足,制氧机工况恶化,通过分析找出了原因是因某单元氧通道堵塞,并采取了处理措施。 相似文献
58.
本文合成了抑制H2S腐蚀的咪唑啉类缓蚀剂,并研究了它们的性能。正交试验表明,最佳合成条件是:反应温度:190~200℃;酸:胺(mol比)=1:1.05;反应时间以3~5小时为宜。根据以上条件合成的缓蚀剂,在含有3200mg/lH2S的泥浆中,用量为0.2%时,抑制效果能达到80~90%,与碱式碳酸锌复配使用时,能提高缓蚀效率和降低碱式碳酸锌的用量,并发现缓蚀剂与CaO之间具有明显的协同效应。泥浆流变性能试验表明,缓蚀剂能提高泥浆的动切力和动塑比,对失水量影响不大。此外,利用X-射线衍射试验证明:(1)低碳钢在含H2S的泥浆中,腐蚀产物的主要组成为Fe9S6;(2)H2S会破坏FCLS的稳定性,分解出Fe2+,与H2S反应生成面心立方的Fe2S4。 相似文献
59.
Jagath C Rajapakse Choong Leong Tan Xuebin Zheng Susanta Mukhopadhyay Kanyan Yang 《IEEE engineering in medicine and biology magazine》2006,25(2):102-111
Covariance-based methods of exploration of functional connectivity of the brain from functional magnetic resonance imaging (fMRI) experiments, such as principal component analysis (PCA) and structural equation modeling (SEM), require a priori knowledge such as an anatomical model to infer functional connectivity. In this research, a hybrid method, combining independent component analysis (ICA) and SEM, which is capable of deriving functional connectivity in an exploratory manner without the need of a prior model is introduced. The spatial ICA (SICA) derives independent neural systems or sources involved in task-related brain activation, while an automated method based on the SEM finds the structure of the connectivity among the elements in independent neural systems. Unlike second-order approaches used in earlier studies, the task-related neural systems derived from the ICA provide brain connectivity in the complete statistical sense. The use and efficacy of this approach is illustrated on two fMRI datasets obtained from a visual task and a language reading task. 相似文献
60.
郑国忠 《中国非金属矿工业导刊》1994,(4)
本文首先讨论GPS星座组成及美国的用政策,阐述了AS措施及其对GPS测量的影响。然后,对抗AS的若干方案进行介绍,分别对平方法、互相关法、码相关干法及P-W技术(Ashtech法)进行叙述和评论,指出它们的优缺点,评述它们在GPS技术发展过程所起的作用。最后,对GPS星座建成后的形势作了小结,并对当前我国GPS事业的有关问题提出四点建议。 相似文献