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The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase. 相似文献
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对两步法合成己二酸二酰肼(ADH)的工艺条件进行了研究。首先采用己二酸和甲醇酯化反应合成了己二酸二甲酯,然后将己二酸二甲酯与水合肼酰肼化,得到了高纯度己二酸二酰肼,并确定了适宜的工艺条件:对于酯化反应,催化剂为KHSO4,用量为己二酸质量数的5.5%,n(醇)/n(酸)为4.0,反应时间1.0 h,在该条件下,产品己二酸二甲酯质量分数高达99.8%,收率为77%;对于酰肼化反应,n(肼)/n(酯)、n(醇)/n(酯)比均为4.0,反应时间为1.5 h,此条件下,ADH收率达99%,质量分数高达99.5%。 相似文献
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通过对结构胶在工业厂房吊车走行部修缮应用实例的介绍与分析,结合工程经验和有关资料提出了合理的加固方法,给同类工程的实施提供了一定的借鉴,也提供了增加既有设施寿命的一种可行、简便的方法。 相似文献
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基于大型通用有限元软件ABAQUS对一钢框架-钢筋混凝土核心筒结构的动力弹塑性性能进行了初步研究,研究了这类结构体系在地震作用下的混凝土损伤机制和框架与核心筒之间的剪力分配等,本文的研究成果可为同类研究提供参考。 相似文献