Haemoglobins of the shark, Heterodontus portusjacksoni II. Amino acid sequence of the alpha-chain

The amino acid sequence of the alpha-chain of the principal haemoglobin from the shark, H. portusjacksoni has been determined. The chain has 148 residues and is acetylated at the amino terminal. The soluble peptides obtained by tryptic and chymotryptic digestion of the protein or its cyanogen bromide fragments were isolated by gel filtration, paper ionophoresis and paper chromatography. The amino acid sequences were determined by the dansyl-Edman procedure. The insoluble "core" peptide from the tryptic digestion contained 34 residues and required cleavage by several prosteases before the sequence was established. Compared with human alpha-chain there are 88 amino acid differences including the additional seven residues which appear on the amino terminal of the shark chain. There is also one deletion and one insertion. The chain contains no tryptophan but has four cysteinyl residues which is the highest number of such residues recorded for a vertebrate globin. In the alpha1beta1 contact sites there are four changes in the oxyhaemoglobin form and six deoxy form. Nine of the 16, alpha1beta1 contact sites show variation while three of the haem contact sites have changed in comparison to the residues known to be involved in these interactions in horse haemoglobin alpha-chain. Use of the sequence data to estimate a time of divergence of the shark from the main vertebrate line yielded the value of 410 +/- 46 million years. The data, in general, support the palaeontological view that bony fishes arose before the elasmobranchs.     

The relationship of education to blood pressure: findings on 40,000 employed Chicagoans

The relationship of education to both actual blood pressure and the prevalence of high blood pressure, based on a systolic pressure of 160 mm Hg or greater or a diastolic pressure of 95 mm Hg or greater, was analyzed among 27,033 men and women, white and black, age 25-44 and 45-64, from the Chicago Heart Association Detection Project in Industry. The educational status of each individual was categorized as not a high school graduate, high school graduate, some college, or college graduate. A statistically significant inverse association between education and high blood pressure was present in all groups of whites. This association could not be "accounted for" by differences in age, relative weight, and heart rate among the educational strata. Controlling for these variables did, however, lessen the association. Among black males a significant inverse association between education level and blood pressure was found for the younger group. For the older black males there was a clear inverse association although with the small numbers it did not achieve statistical significance. For black females there was no clear association.     

Influence of the common varistor dopants (CoO, Cr2O3 and Nb2O5) on the structural properties of SnO2 ceramics

The influence of dopants commonly used in SnO₂ varistor ceramics, such as CoO, Cr₂O₃ or Nb₂O₅, on the structural properties of SnO₂ was investigated. Several SnO₂-based ceramics containing only one of the dopants were prepared and characterized. Spectroscopic investigations [visible, near infrared (IR) and IR region] were performed to obtain information about dopants valence states inside the ceramics, as well as about their influence on electronic structure of the material. Structural properties were investigated by X-ray diffraction analysis and mechanisms of dopant incorporation were proposed. Obtained results were confirmed with results of the electrical measurements. Microstructural changes in doped ceramics were investigated by scanning electron microscopy (SEM) analysis that showed great differences in densities, grain size, and morphology of the SnO₂ ceramics depending on type of dopants and their distribution.     

Towards ubiquity in ambient intelligence: User-guided component mobility in the HI architecture

Ambient Intelligence (AmI) systems need to be as transparent as possible, that is, their users should perceive only the effects of the features presented to them and, in some cases, some kind of interface. They should not be conscious of how these features are implemented, from a hardware or from a software point of view.     

Structural Refinement and Photoluminescence Properties of MnWO4 Nanorods Obtained by Microwave-Hydrothermal Synthesis

Manganese tungstate (MnWO₄) nanorods were prepared at room temperature by the co-precipitation method and synthesized after processing in a microwave-hydrothermal (MH) system at 140 °C for 6–96 min. These nanorods were structurally characterized by X-ray diffraction (XRD), Rietveld refinements and Fourier transform (FT)-Raman spectroscopy. The growth direction, shape and average size distribution of nanorods were observed by means of transmission electron microscopy (TEM) and high resolution TEM (HR-TEM). The optical properties of the nanorods were investigated by ultraviolet visible (UV-vis) absorption and photoluminescence (PL) measurements. XRD patterns, Rietveld refinement data and FT-Raman spectroscopy indicate that the MnWO₄ precipitate is not a single phase structure while the nanorods synthesized by MH processing have a wolframite-type monoclinic structure without deleterious phases. FT-Raman spectra exhibited the presence of 17 Raman-active modes from 50 to 1,000 cm⁻¹. TEM and HR-TEM micrographs indicated that the nanorods are aggregated due to surface energy by Van der Waals forces and grow along the [100] direction. UV–vis absorption measurements confirmed non-linear values for the optical band gap (from 3.2 to 2.72 eV), which increased as the MH processing time increased. The structural characterizations indicated that the presence of defects in the MnWO₄ precipitate promotes a significant contribution to maximum PL emission, while MnWO₄ nanorods obtained by MH processing decrease the PL emission due to the reduction of defects in the lattice.     

Hydroxyapatite growth on cotton fibers modified chemically

We have prepared carboxymethyl cellulose fibers (CMC) by chemically modifying cotton cellulose with monochloroacetic acid and calcium chloride solution. This modification favored the growth of hydroxyapatite (HAP) on the surface of the CMC fibers in contact with simulated body fluid solutions (SBF). After soaking in SBF for periods of 7, 14 and 21 days, formation of HAP was observed. Analysis by scanning electron microscopy and X-ray diffraction showed that crystallinity, crystallite size, and growth of HAP increased with the soaking time. The amount of HAP deposited on CMC fibers increased greatly after 21 days of immersion in the SBF, while the substrate surface was totally covered with hemispherical aggregates with the size of the order of 2 microns. Elemental analysis showed the presence of calcium and phosphate, with calcium/phosphate atomic ratio of 1.54. Fourier transform infrared spectroscopy bands confirmed the presence of HAP. The results suggest that cotton modified by calcium treatment has a nucleating ability and can accelerate the nucleation of HAP crystals.     

Automatic adjustment of a medical imaging data acquisition system to unknown delays in the input communication channels

In this article, novel FIFO and RAM-based Synchronization Modules to keep synchronism throughout the input channels of a Data Acquisition Electronics (DAE) system are proposed. DAE is a main component of a Medical Imaging System, namely, a Positron Emission Mammography (PEM) system. DAE input data comes from a scanner constituted by an array of scintillating crystals. The scanner captures radiation generated by human cells injected with a radioactive substance and converts it into electrical signals. The corresponding digital information is sent to the DAE. In order to deal with the huge amount of data, flowing at high data rates, point-to-point (p2p) communication channels are used between the scanner and the DAE. Propagation delays associated with the different communication channels may change differently. Additionally, differences among channel delays may exceed one clock period. Keeping synchronism in these circumstances requires more than the classical asynchronous FIFO solution. All these aspects motivate the work proposed in this article. The PEM DAE system is a multi-board, multi-FPGA, multi-clock domain system. Therefore, the DAE architecture follows a Globally Asynchronous, Locally Synchronous (GALS) design style. The novel Synchronization Modules proposed in this article are implemented in the DAE. The effectiveness of these new structures is validated through simulation and laboratorial test. Simulation and test results are presented.     

New functional fibre in milk puddings: Effect on sensory properties and consumers' acceptability

The effect of the addition of a new functional fibre (high-amylose maize starch, HAMS, as a source of resistant starch), recently available in the market, on sensory characteristics and consumers' acceptability of milk puddings was studied. Milk puddings containing modified waxy maize starch and κ-carrageenan were produced with different HAMS concentrations (0, 1, 2, 3 and 4%). Higher HAMS concentration caused changes in the sensory characteristics of milk puddings. Particularly, sensory attributes such as some roughness, rough afterfeel and floury taste appeared. Besides, the addition of HAMS caused an increase in manual and oral thickness and a decrease in creaminess, melting, and sweetness. A HAMS enrichment level of 1.4% in this product was estimated as the maximum concentration that does not significantly modify consumers' overall acceptability. Using survival analysis the proportion of consumers who would buy milk desserts containing 1.4 % HAMS was estimated as 71%. Consumers more interested in consuming functional foods enriched with fibre were more tolerant to the sensory changes caused by the addition of HAMS to the milk puddings.