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71.
Bhattacharya D Saha S Basu S Chakravarty S Chakravarty A Banerjee D Chakrabarti A 《Proteomics. Clinical applications》2010,4(5):480-488
Purpose: In (hemoglobin, Hb) HbEβ‐thalassemia, HbE (β‐26 Glu→Lys) interacts with β‐thalassemia to produce clinical manifestation of varying severity. This is the first proteomic effort to study changes in protein levels of erythrocytes isolated from HbEβ‐thalassemic patients compared to normal. Experimental design: We have used 2‐DE and MALDI‐MS/MS‐based techniques to investigate the differential proteome profiling of membrane and Hb‐depleted fraction of cytosolic proteins of erythrocytes isolated from the peripheral blood samples of HbEβ‐thalassemia patients and normal volunteers. Results: Our study showed that redox regulators such as peroxiredoxin 2, Cu‐Zn superoxide dismutase and thioredoxin and chaperones such as α‐hemoglobin stabilizing protein and HSP‐70 were upregulated in HbEβ‐thalassemia. We have also observed larger amounts of membrane associated globin chains and indications of disruption of spectrin‐based junctional complex in the membrane skeleton of HbEβ‐thalassemic erythrocytes upon detection of low molecular weight fragments of β‐spectrin and decrease in β‐actin and dematin content. Conclusion and clinical relevance: We have observed interesting changes in the proteomic levels of redox regulators and chaperons in the thalassemic hemolysates and have observed strong correlation or association of the extent of such proteomic changes with HbE levels. This could be important in understanding the role of HbE in disease progression and pathophysiology. 相似文献
72.
73.
In this paper, we propose a numerical scheme which is almost second-order spatial accurate for a one-dimensional singularly perturbed parabolic convection-diffusion problem exhibiting a regular boundary layer. The proposed numerical scheme consists of classical backward-Euler method for the time discretization and a hybrid finite difference scheme for the spatial discretization. We analyze the scheme on a piecewise-uniform Shishkin mesh for the spatial discretization to establish uniform convergence with respect to the perturbation parameter. Numerical results are presented to validate the theoretical results. 相似文献
74.
This paper investigates the sensitivity improvement of an air filled cavity based thermal accelerometer. The accelerometer does not contain any solid proof mass and it is realizable in CMOS technology. The sensitivity has been improved by a new square ring shaped heater structure. The sensitivity of accelerometer performance is compared with other different heater shapes. It is found that, heater design having higher heat dissipation in the suspended beam gives higher sensitivity. The simulation is carried out using commercial FEM simulator COMSOL Multiphysics. For the peak heater temperature of 609 K, the proposed square ring shaped heater provides a sensitivity of 0.335 K/g. Under same conditions, sensitivity of an accelerometer with a meander shaped heater structure is only 0.098 K/g and diamond shaped structure is 0.229 K/g. 相似文献
75.
This paper deals with the study of a post-processing technique for one-dimensional singularly perturbed parabolic convection–diffusion
problems exhibiting a regular boundary layer. For discretizing the time derivative, we use the classical backward-Euler method
and for the spatial discretization the simple upwind scheme is used on a piecewise-uniform Shishkin mesh. We show that the
use of Richardson extrapolation technique improves the ε-uniform accuracy of simple upwinding in the discrete supremum norm from O (N
−1 ln N + Δt) to O (N
−2 ln2
N + Δt
2), where N is the number of mesh-intervals in the spatial direction and Δt is the step size in the temporal direction. The theoretical result is also verified computationally by applying the proposed
technique on two test examples. 相似文献
76.
A stability-based distributed routing mechanism to support unicast and multicast routing in ad hoc wireless network 总被引:6,自引:0,他引:6
An ad hoc network can be envisioned as a collection of mobile routers, each equipped with a wireless transceiver, which are free to move about arbitrarily. In ad hoc wireless networks, even if two nodes are outside the wireless transmission range of each other, they may still be able to communicate in multiple hops using other intermediate nodes. However, the dynamics of these networks, as a consequence of mobility and disconnection of mobile hosts, pose a number of problems in designing routing schemes for effective communication between any pair of source and destination. In this paper, a stability-based unicast routing mechanism, that considers both link affinity and path stability in order to find out a stable route from source to destination, is proposed. It is then extended to support multicast routing as well where only local state information (at source) is utilized for constructing a multicast tree. The performance of the proposed scheme is evaluated on a simulated environment to show that the stability-based scheme provides a unified framework for both unicast and multicast routing and reduces the probability of route error drastically in both the cases. 相似文献
77.
78.
The creep behaviour of polyvinyl chloride (PVC) has been studied in the temperature range 280 to 340° F under constant stress varying from 3.4 to 22.7 p.s.i. It is shown that the steady-state creep rate is an exponential function of stress as suggested by Norton but the exponent decreases with temperature. The activation energy for creep is determined using an activated-state rate process and it is found to be a decreasing function of stress with a higher value at temperatures 320° F and above. It is shown that the time dependent strain can be represented by $$\gamma = \gamma _0 + \dot \gamma _s t + \gamma _T \left[ {1 - \exp \left( { - K\dot \gamma _s t} \right)} \right]$$ where γ 0 is the instantaneous strain on stressing, \(\dot \gamma _s\) the secondary creep rate, γ T transient strain, and K is a constant. Scanning electron micrograph studies of the fracture surface and the change in activation energy apparently support the probability of two different deformation mechanisms i.e., domain flow and chain segmental or molecular flow at temperatures below and above 320° F, respectively. 相似文献
79.
Multicomponent silicate glasses of composition (wt%) 66SiO2-18B2O3-7Al2O3-6Na2O-3BaO were prepared by three sol-gel processes which differed primarily in the extent of hydrolysis of the metal alkoxide precursors. Gels which were prepared from solutions in which a stoichiometric excess of water was added, causing extensive replacement of OR groups (R (C
x
H(2x+1))s–1 by hydroxyl groups, were converted to fully dense, organic-free, monolithic glasses at temperatures near the glass transition temperature. Gels containing large numbers of OR groups showed enhanced densification at lower temperatures due to condensation reactions, but these gels could not be converted to fully dense, organic-free glasses. This investigation has shown that at least three possible densification mechanisms might be operative during the gel to glass conversion: volume relaxation, condensation reactions and viscous sintering.A US Department of Energy Facility. 相似文献
80.
The temporal scale effect of loading data on nitrate-nitrogen load computation was examined using outputs of watershed modeling tool Hydrologic Simulation Program-FORTRAN (HSPF) for the Amite River in Louisiana, USA. The daily nitrate-nitrogen concentrations simulated using the HSPF were employed first to obtain daily, weekly, bi-weekly, and monthly average data and then to develop load duration curves for the data with four different temporal scales. The duration curves exhibited high variability in the load estimated using daily data as compared with those based on bi-weekly and monthly data. According to daily data, the nitrate-nitrogen load in the winter was found to be 2,780 kg. The nitrate-nitrogen load decreased with increasing temporal (daily, weekly, bi-weekly, and monthly) scale (commonly used in water quality monitoring) of the data. The coefficient of variation, used to quantify the effect of temporal scale on the load, was found to be linearly and inversely correlated with the logarithm of the time scale. Based on the finding, empirical equations were proposed to extrapolate near real-time data for flow and nitrate-nitrogen, greatly simplifying nutrient monitoring and reducing the cost involved in water quality monitoring. 相似文献