Isolation of adipophilin and butyrophilin from bovine milk and characterization of a cDNA encoding adipophilin

The milk fat globule membrane-associated proteins adipophilin (alias adipocyte differentiation-related protein) and butyrophilin were purified from bovine milk by reverse-phase chromatography. The nucleotide sequence of bovine adipophilin was obtained via peptide mapping and sequencing of a mammary gland cDNA clone, which comprises 1841 nucleotides and has an open reading frame of 450 amino acids. By peptide mapping, 19% of the amino acid sequence was confirmed. The obtained amino acid sequence has 87 and 80% identical residues with human and mouse adipophilin, respectively. Alignment with the proteins perilipin and TIP47 revealed two highly conserved segments, which may assemble into amphipathic alpha-helices.     

Mobility Impact on Cluster Based MAC Layer Protocols in Wireless Sensor Networks

Wireless sensor networks (WSNs) enable a wide variety of applications resulting in still increasing requirements for the protocols supporting the operations. The medium access control (MAC) layer protocols are essential for improving the performance of an application and its quality of service because MAC protocols influence channel capacity utilization, network delay, energy consumption, and scalability. The contribution of this paper is two novel cluster-based time division multiple access (TDMA) scheduling MACs for WSNs and an analysis of the mobility impact on both. The proposed MAC layer protocols support real time applications where the cluster-based scheduling improves the scalability and also improves the performance in varying network conditions. The paper presents the design, implementation and performance evaluation of the proposed cluster based TDMA scheduling algorithms green conflict free (GCF) and multicolor-GCF (M-GCF) for high complexity and high requirement applications of WSNs under both low and high mobility conditions. The comparative evaluation shows that the M-GCF algorithm has better slot sharing and less conflicts with reduced communication energy consumption, delay, and good throughput under static and low mobility conditions while the GCF algorithm has better performance in high mobility scenarios. The paper also defines the mobility threshold that decides the use of the GCF- and M-GCF algorithms according to the mobility requirement of application.     

Room electromagnetics

In analogy with the established discipline of room acoustics, various aspects of diffuse wideband microwave propagation in a room are treated. It is shown that an equivalent to Sabine's equation for reverberation time in a room is valid for the completely diffused field, depending only on the volume, the surface area, and an effective absorption coefficient. An exponential decay of the power as a function of the delay is a consequence of the assumptions. Furthermore, the concept of a reverberation distance is also valid. This is the distance from a transmitting antenna where the received diffuse, randomly scattered power equals the direct line-of-sight received power, such that the diffuse power dominates for distances larger than the reverberation distance. A number of measurements in a large room support the theory with an effective absorption coefficient of 0.5. The power delay profiles around the room from a transmitter in the ceiling vary only in the first arriving part of the impulse, whereas the tail, being dominated by the diffuse field, has the same power level for a given delay and the same decay rate all over the room. It is also a consequence of the theory that the diffuse fields incident on an antenna are uniformly distributed in angle.     

A Transmitter Architecture Based on Delta–Sigma Modulation and Switch-Mode Power Amplification

This brief presents a method of deploying RF switch-mode power amplification for varying envelope signals. Thereby the power amplifier can be operated as a switch with a high power efficiency as the result. The key idea is to transmit either a full RF period or none at all in such a way that the correct modulated RF signal is obtained after filtering. This is accomplished in a novel configuration of a low-pass DeltaSigma modulator using a phase modulated clock combined with a simple AND-gate. The designed modulator is easy to implement, displays very good linearity and offers time domain signals that promote the power efficiency of the power amplifier. The working principle is described through theory and simulations, and validation is done via measurements on a prototype of the modulator. Measurements on the prototype show that the presented modulator modulates a UMTS signal with more than 10-dB margin to the spectrum mask and EVM below 0.85% RMS (req<17.5%). Delta-sigma, power amplifier (PA), RF, switch mode, transmitter architecture, varying envelope.     

UNICORE 6 — Recent and Future Advancements

UNICORE is a European Grid Technology with more than 10 years of history. Originating from the Supercomputing domain, the latest version UNICORE 6 has turned into a general-purpose Grid technology that follows established standards and offers a rich set of features to its users. The paper starts with an architectural insight into UNICORE 6, highlighting the workflow features, standards and the different clients. Next, the current state of advancement is presented by describing recent developments. The paper closes with an outlook on future planned developments.     

Low‐Temperature Superionic Conductivity in Strained Yttria‐Stabilized Zirconia

Very high lateral ionic conductivities in epitaxial cubic yttria‐stabilized zirconia (YSZ) synthesized on single‐crystal SrTiO₃ and MgO substrates by reactive direct current magnetron sputtering are reported. Superionic conductivities (i.e., ionic conductivities of the order ～1 Ω^?1cm^?1) are observed at 500 °C for 58‐nm‐thick films on MgO. The results indicate a superposition of two parallel contributions – one due to bulk conductivity and one attributable to conduction along the film–substrate interface. Interfacial effects dominate the conductivity at low temperatures (<350 °C), showing more than three orders of magnitude enhancement compared to bulk YSZ. At higher temperatures, a more bulk‐like conductivity is observed. The films have a negligible grain‐boundary network, thus ruling out grain boundaries as a pathway for ionic conduction. The observed enhancement in lateral ionic conductivity is caused by a combination of misfit dislocation density and elastic strain in the interface. These very high ionic conductivities in the temperature range 150–500 °C are of great fundamental importance but may also be technologically relevant for low‐temperature applications.     

Self‐Assembly of Mechanoplasmonic Bacterial Cellulose–Metal Nanoparticle Composites

Nanocomposites of metal nanoparticles (NPs) and bacterial nanocellulose (BC) enable fabrication of soft and biocompatible materials for optical, catalytic, electronic, and biomedical applications. Current BC–NP nanocomposites are typically prepared by in situ synthesis of the NPs or electrostatic adsorption of surface functionalized NPs, which limits possibilities to control and tune NP size, shape, concentration, and surface chemistry and influences the properties and performance of the materials. Here a self‐assembly strategy is described for fabrication of complex and well‐defined BC–NP composites using colloidal gold and silver NPs of different sizes, shapes, and concentrations. The self‐assembly process results in nanocomposites with distinct biophysical and optical properties. In addition to antibacterial materials and materials with excellent senor performance, materials with unique mechanoplasmonic properties are developed. The homogenous incorporation of plasmonic gold NPs in the BC enables extensive modulation of the optical properties by mechanical stimuli. Compression gives rise to near‐field coupling between adsorbed NPs, resulting in tunable spectral variations and enhanced broadband absorption that amplify both nonlinear optical and thermoplasmonic effects and enables novel biosensing strategies.     

Distributed cross-layer coordination of congestion control and resource allocation in S-TDMA wireless networks

We consider the problem of joint congestion control and resource allocation in spatial-TDMA wireless networks. The design problem is posed as a utility maximization problem subject to link rate constraints which involve both transmission scheduling and power allocation. Starting from the performance limitations of a centralized optimization based on global network information, we proceed systematically in our development of two distributed and transparent protocols that rely on local information only. In the process, we introduce a novel decomposition method for convex optimization, establish its convergence for the utility maximization problem, and demonstrate how it suggests a distributed solution based on TCP/AQM and incremental updates of the transmission schedule. We develop a two-step procedure for finding the schedule updates and suggest two schemes for distributed link scheduling and power control under realistic interference models. Although the final protocols are suboptimal, we isolate and quantify the performance losses incurred by each simplification and demonstrate strong performance in examples.

Overview of the MPEG Reconfigurable Video Coding Framework

Video coding technology in the last 20 years has evolved producing a variety of different and complex algorithms and coding standards. So far the specification of such standards, and of the algorithms that build them, has been done case by case providing monolithic textual and reference software specifications in different forms and programming languages. However, very little attention has been given to provide a specification formalism that explicitly presents common components between standards, and the incremental modifications of such monolithic standards. The MPEG Reconfigurable Video Coding (RVC) framework is a new ISO standard currently under its final stage of standardization, aiming at providing video codec specifications at the level of library components instead of monolithic algorithms. The new concept is to be able to specify a decoder of an existing standard or a completely new configuration that may better satisfy application-specific constraints by selecting standard components from a library of standard coding algorithms. The possibility of dynamic configuration and reconfiguration of codecs also requires new methodologies and new tools for describing the new bitstream syntaxes and the parsers of such new codecs. The RVC framework is based on the usage of a new actor/ dataflow oriented language called CAL for the specification of the standard library and instantiation of the RVC decoder model. This language has been specifically designed for modeling complex signal processing systems. CAL dataflow models expose the intrinsic concurrency of the algorithms by employing the notions of actor programming and dataflow. The paper gives an overview of the concepts and technologies building the standard RVC framework and the non standard tools supporting the RVC model from the instantiation and simulation of the CAL model to software and/or hardware code synthesis.     

Multi-User Relaying of High-Rate Space–Time Code in Cooperative Networks

In this paper, we propose high-rate space–time coding for cooperative wireless networks to reduce the overall delay incurred in relaying signals to multiple receivers. The relay structure is optimized in order to achieve maximum SNR at the receiver nodes. The proposed scheme provides a significant reduction in the delay required for the relaying and transmission of the signals to the multiple receivers with a minute loss in performance. We have also shown by simulation that this loss in the performance could be recovered by selecting more number of relays. We propose two relaying strategies for high-rate space–time codes, which are very useful in providing high data-rate in wireless cooperative networks.