Isolation and Characterisation of a Ferrirhodin Synthetase Gene from the Sugarcane Pathogen Fusarium sacchari

FSN1, a gene isolated from the sugar‐cane pathogen Fusarium sacchari, encodes a 4707‐residue nonribosomal peptide synthetase consisting of three complete adenylation, thiolation and condensation modules followed by two additional thiolation and condensation domain repeats. This structure is similar to that of ferricrocin synthetase, which makes a siderophore that is involved in intracellular iron storage in other filamentous fungi. Heterologous expression of FSN1 in Aspergillus oryzae resulted in the accumulation of a secreted metabolite that was identified as ferrirhodin. This siderophore was found to be present in both mycelium and culture filtrates of F. sacchari, whereas ferricrocin is found only in the mycelium, thus suggesting that ferricrocin is an intracellular storage siderophore in F. sacchari, whereas ferrirhodin is used for iron acquisition. To our knowledge, this is the first report to characterise a ferrirhodin synthetase gene functionally.     

黄色云母珠光颜料的制备

将纳米BiVO4包覆在云母钛珠光颜料上,制得了黄色珠光颜料。介绍了该珠光颜料的制备方法,并探讨了BiVO4的包覆机理。     

球墨铸铁气焊工艺探讨

球墨铸铁因其铸造性能差,常产生缩孔、缩松、夹渣、石墨漂浮、皮下气孔以及球化衰退等缺陷[1,2],其中缩孔、缩松和夹渣缺陷往往是球墨铸铁件的致命缺陷,相当一部分铸件因为局部位置的缺陷而报废.公司现有的球墨铸铁气焊材料为401焊丝和402焊丝,大部分焊接后有色差要求的球墨铸铁件不能挽救.本文通过对球墨铸铁件气焊焊材的选择和工艺探讨,以期能够对缺陷铸件挽救工程提供帮助.     

磁场形态对等离子弧堆焊层组织性能的影响

在低碳钢表面进行Fe5自熔合金等离子弧堆焊时外加纵向磁场,并改变磁场形态,对不同磁场参数下堆焊试样进行硬度和磨损试验.采用显微电镜和扫描电镜对堆焊层显微组织进行分析,研究磁场形态和参数对堆焊层组织性能的影响规律和作用机理.结果表明,交、直流纵向磁场均可以改善堆焊层的组织性能,由于交流纵向磁场中频率可调,增加了磁场的可控性,对堆焊层组织性能的作用效果比直流纵向磁场更明显.在焊接电流为160A,磁场电流为3A,磁场频率为10Hz时,堆焊层金属性能达到最佳值,此时硬度为68HRC,磨损量为0.0318g.     

The Complex and Critical Role of Glycine 12 (G12) in Beta-Connexins of Human Skin

Glycine is an amino acid with unique properties because its side chain is composed of a single hydrogen atom. It confers conformational flexibility to proteins and conserved glycines are often indicative of protein domains involving tight turns or bends. All six beta-type connexins expressed in human epidermis (Cx26, Cx30, Cx30.3, Cx31, Cx31.1 and Cx32) contain a glycine at position 12 (G12). G12 is located about halfway through the cytoplasmic amino terminus and substitutions alter connexin function in a variety of ways, in some cases altering protein interactions and leading to cell death. There is also evidence that alteration of G12 changes the structure of the amino terminus in connexin- and amino acid- specific ways. This review integrates structural, functional and physiological information about the role of G12 in connexins, focusing on beta-connexins expressed in human epidermis. The importance of G12 substitutions in these beta-connexins is revealed in two hereditary skin disorders, keratitis ichthyosis and erythrokeratodermia variabilis, both of which result from missense mutations affecting G12.     

不同污泥干化焚烧技术路线全链条碳足迹分析

碳足迹分析是目前国际上常用的技术能耗评估和资源优化配置的方法.文中采用IPCC《国家温室气体清单指南》提供的方法,对干化-焚烧、深度脱水-干化-焚烧、厌氧消化-深度脱水-干化-焚烧3种基于焚烧的污泥处理处置技术路线进行碳足迹分析.结果表明,污泥干化-焚烧技术路线净碳排放量最低.厌氧消化虽然是污泥资源化利用的重要方式,但...     

污水处理工艺反硝化过程的不同碳源研究

研究了污水处理厂三种常用碳源-乙酸、乙酸钠和葡萄糖对污水处理厂反硝化作用的影响。实验结果表明使用这三种碳源时,对污水处理厂在反硝化过程中均无明显的亚硝酸盐氮累积。去除单位质量硝酸盐氮所需的乙酸质量最少,葡萄糖最高。乙酸、乙酸钠和葡萄糖三种碳源对反硝化速率的表现为乙酸>乙酸钠>葡萄糖。使用乙酸和乙酸钠作为碳源时,会导致出水pH值升高;而使用葡萄糖时则表现为pH值降低。     

Optimized excitation energy transfer in a three-dye luminescent solar concentrator

The spectral range of sunlight absorbed by a luminescent solar concentrator (LSC) is increased by using multiple dyes. Absorption, fluorescence, and fluorescence excitation spectra, and relative light output are reported for LSCs made with one, two, or three BODIPY dyes in a thin polymer layer on glass. Losses caused by multiple emission and reabsorption events are minimized by optimizing resonance excitation energy transfer between dyes. Increases in the outputs from the multiple-dye LSCs are directly proportional to increases in the number of photons absorbed. The output of the three-dye LSC is 45–170% higher than those of the single-dye LSCs.     

Estimation of the protein content of US imports of milk protein concentrates

Recent declines in milk prices in the United States have sparked renewed concern that imports of milk protein concentrates (MPC) are increasingly entering the United States with very low tariff rates and is having an adverse impact on the US dairy industry. Milk protein concentrates are used in the United States in many different products, including the starter culture of cheese, or in nonstandard cheeses such as baker's cheese, ricotta, Feta and Hispanic cheese, processed cheese foods, and nutritional products. One of the difficult aspects of trying to assess the impact of MPC imports on the US dairy industry is to quantify the protein content of these imports. The protein content of MPC imports typically ranges from 40 to 88%. The purpose of this study is to develop a methodology that can be used to estimate the protein content of MPC on a country by country basis. Such an estimate would not only provide information regarding the quantity of protein entering the United States, but would also provide a profile of low- and high-value MPC importers. This is critical for market analysis, since it is the lower valued MPC imports that more directly displaces US-produced skim milk powder.