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131.
O-Alkyl N-anthracenylmethyl derivatives of Cinchona alkaloids can function as enantioselective phase-transfer catalysts. By employing these catalysts in the asymmetric alkylation of glycine imines, one can generate a range of alpha-amino acid derivatives with high levels of enantiomeric excess. It is also possible to generate the catalysts in situ from commercially available chiral amines, which offers the opportunity to evaluate libraries of related structures. This latter approach has been successfully applied to a series of biphenyl quaternary ammonium salts resulting in the development of a new highly selective catalyst and opening up the potential of further expanding the range of alpha-amino acid derivatives that can be prepared. 相似文献
132.
This work describes a system for precise re-location of cells within a monolayer after atomic force imaging. As we know little about probe interaction with soft biological surfaces any corroborative evidence is of great importance. For example, it is of paramount importance in living cell force microscopy that interrogated cells can be re-located and imaged by other corroborative technologies. Methodologies expressed here have shown that non-invasive force parameters can be established for specific cell types. Additionally, we show that the same sample can be transferred reliably to an SEM. Results here indicate that further work with live cells should initially establish appropriate prevailing force parameters and that cell damage should be checked for before and after an imaging experiment. 相似文献
133.
Previous research has classified the occupation of sheep shearing as heavy work where shearers flex their spine and hips for long periods of time, handle awkward loads and expend high amounts of energy. The aim of this research was to investigate the magnitude of spinal forces produced during the shearing phase of the work and to determine whether the use of a commercially available back support harness would reduce these forces. Following discussion on task complexity and risk of back injury with senior shearing instructors, three component tasks of the shearing phase were identified as posing high risk of injury and were prioritized for primary analysis. Although the dragging out of a sheep in preparation for shearing and an unexpected loss of animal control were also identified as being of high risk, technological and instrumentation difficulties precluded their analysis. Twelve experienced shearers were videotaped while shearing with and without the use of a back harness. Surface mounted retro-reflective markers placed on the trunk defined three linked segments: Pelvis, Lumbar and Head, Arms, and Upper Trunk (HAUT). A 3D, link segment, top down, inverse dynamics approach was used to describe the motion and to estimate forces involved during the identified tasks of shearing. The spinal force/time profiles of this sample of shearers demonstrated large compressive and shear forces for all three tasks that are close to the NIOSH and University of Waterloo action limits for compressive and shear forces respectively (McGill 1997, Yingling and McGill 1999, Marras 2000). The use of the back support harness reduced these forces by substantial and statistically significant amounts. This effect was consistent across all three tasks. The results of this study demonstrate the production of high levels of compressive and shear forces within the spine of shearers during the three shearing tasks studied and that the use of a back support harness can substantially reduce these forces. Therefore the use of a back harness may reduce the cumulative load on the spine during shearing thereby moderating damage to the articular structures. However it is not known whether the harness would protect the spine from a sudden or unexpected force. 相似文献
134.
This paper presents application of sequential enhanced data processing procedures to high-resolution tandem mass spectra for identification of peptides using the Mascot database search algorithm. A strategy for (1) selection of fragment ion peaks from MS/MS spectra, (2) utilization of improved mass accuracy of the precursor ions, and (3) wavelet denoising of the mass spectra prior to fragment ion selection have been developed. The number of peptide identifications obtained using the enhanced processing was then compared with that obtained using software provided by the instrument manufacturer. Approximately 9000 MS/MS spectra acquired by the Applied Biosystems 4700 TOF/TOF MS instrument were used as a model data set. After application of the new processing, an increase of 33% unique peptides and 22% protein identifications with at least two unique peptides were found. The influence of the processing on the percentage of false positives, estimated by searching against a randomized database, was estimated to increase false positive identifications from 2.7 to 3.9%, which was still below the 5% error rate specified in the Mascot search. These data processing approaches increase the amount of information that can be extracted from LC-MS analysis without the necessity of additional experiments. 相似文献
135.
Fluorescence has been suggested as a method with which to detect and identify bacterial spores. To better understand the nature of the fluorescence signal, we observed the intrinsic steady-state fluorescence and phosphorescence spectra of Bacillus globigii (BG) in both dried and aqueous forms. In vitro, dried, and suspension forms of BG were measured at room temperature in 300-600-nm excitation wavelengths. Also, the phosphorescence of dry BG spores was measured at room temperature at 300-600-nm excitation wavelengths. The wet BG spores exhibited a strong maximum in their fluorescence spectrum, with the peak excitation wavelength near 300 nm and emission wavelength near 400 nm. When the BG was dried, this peak shifted to an approximately 450-nm excitation maximum and an 500-nm emission maximum. The difference between the wet and the dry spore fluorescence spectra cannot be explained by the phosphorescence of the dry spores. Other changes must take place when the spores are wet to account for the large changes observed in the spectrum. 相似文献
136.
137.
Barry N. Haack 《Remote sensing of environment》1983,13(3):265-275
Thematic mapper simulator data collected for the Los Angeles Basin in 1980 were examined to assess their utility for urban and near-urban land-cover delimitations. Spectral data for six of the thematic mapper channels were reprojected to a UTM grid and aggregated to 30-m resolution, 120 m for the thermal band. Statistics for 21 training sites representing 8 land-cover types were obtained and examined using transformed divergence calculations for intraclass variability, optimal number of channels for classification, and best channels for classification. Four channels of data are adequate for classification with the best results obtained by selection of one channel from each of the available major portions of the electromagnetic spectrum. The thermal channel data is useful for urban land-cover delineations at 30-m resolution, but its utility at 120-m resolution is not clear from this study. 相似文献
138.
D. P. Saccuzzo and D. L. Schubert (see record 1981-28173-001) proposed that schizophrenic individuals have deficient posticonic processes. The deficiency accounts for the observed general slowness in visual information processing. It is argued that a major methodological mistake in the choice of target durations in a backward masking task invalidates Saccuzzo and Schubert's conclusions. (1 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
139.
Low-glycemic-load diets: impact on obesity and chronic diseases 总被引:3,自引:0,他引:3
Historically, carbohydrates have been thought to play only a minor role in promoting weight gain and in predicting the risk of development of chronic disease. Most of the focus had been on reducing total dietary fat. During the last 20 years, fat intake decreased, while the number of individuals who were overweight or developed a chronic conditions have dramatically increased. Simultaneously, the calories coming from carbohydrate have also increased. Carbohydrates can be classified by their post-prandial glycemic effect, called the glycemic index or glycemic load. Carbohydrates with high glycemic indexes and high glycemic loads produce substantial increases in blood glucose and insulin levels after ingestion. Within a few hours after their consumption, blood sugar levels begin to decline rapidly due to an exaggerated increase in insulin secretion. A profound state of hunger is created. The continued intake of high-glycemic load meals is associated with an increased risk of chronic diseases such as obesity, cardiovascular disease, and diabetes. In this review, the terms glycemic index and glycemic load are defined, coupled with an overview of short- and long-term changes that occur from eating diets of different glycemic indexes and glycemic loads. Finally, practical strategies for how to design low-glycemic-load diets consisting primarily of low-glycemic carbohydrates are provided. 相似文献
140.
Kirwan B 《Applied ergonomics》2003,34(5):441-452
This paper presents a case study of a large Human Factors programme applied in the nuclear fuel reprocessing industry (1987-1991). The paper outlines the key Human Factors issues addressed, as well as the impacts achieved, and gives an indication of the resources utilised (approximately 15 person-years of effort). It also considers the starting point of the programme, in terms of the factors that led to the need for such an extensive programme. Some general lessons learned are given at the end of the paper. 相似文献