High-volume aspiration as a supplemental scavenging method for reducing ambient nitrous oxide levels in the operatory: a laboratory study

Occupational exposure to low levels of nitrous oxide (N2O) have been associated with adverse health effects. The National Institute for Occupational Safety and Health has established a threshold guideline of 25 ppm N2O. The purpose of this laboratory study was to determine the effectiveness of a high-volume dental aspirator as a supplemental device to reduce ambient N2O levels in the operatory. The investigation evaluated four experimental groups that were assigned based on whether or not the aspirator was used and on the rate at which operatory ventilation was established (5 or 10 room air exchanges per hour). Ambient N2O levels were monitored at 30 cm from the nasal hood using an infrared spectrophotometer. The room air exchange rate was measured with a flow hood and then manipulated to the desired ventilation rate. N2O levels were detected with a spectrophotometer and data were recorded with a microprocessor that continuously collected data. The results demonstrated that both utilization of supplemental oral aspiration and increased operatory ventilation significantly reduced ambient N2O levels. It was concluded that a high-volume aspirator, when used in conjunction with the normal scavenging system, can significantly reduce ambient N2O levels to within the guidelines established by the National Institute for Occupational Safety and Health.     

Detection of apoptosis in human and rat ovarian follicles

OBJECTIVE: The purpose of this study was to determine whether cells acquired from individual human preovulatory follicles undergo apoptosis (physiologic cell death) and, if so, to correlate the degree of apoptosis with characteristics of the follicles or the oocytes derived from the follicles. METHODS: We devised a sensitive nonradioactive method for detecting apoptotic DNA fragmentation in small numbers of cells derived from rat atretic follicles and follicular aspirates of patients undergoing assisted reproductive technologies. RESULTS: Using this method, apoptotic DNA was detected in rat atretic follicles, with optimal detection at 10-100 ng. Furthermore, apoptotic DNA was detected in some, but not all individual human follicular aspirates from several patients, and was found in follicles that produced oocytes that fertilized and developed into embryos. CONCLUSION: Apoptosis occurs in cells from human ovarian preovulatory follicles and may be a normal physiologic process of the follicle during luteinization.     

Cloning and assembly of PCR products using modified primers and DNA repair enzymes

We present a method for the creation of ligatable 3' overhangs by the incorporation of a modified base, uracil, at a specific position in the PCR primer and subsequent treatment with the DNA-modifying enzyme uracil DNA glycosylase and then either T4 endonuclease V or human apurinic/apyrimidinic endonuclease 1. In this study, we describe the cloning of a fragment specifying the chloramphenicol-resistance gene into a SacI vector site. To further test this method, three segments of the lacZ gene were amplified by PCR, and after treatment with the DNA-modifying enzymes, the properly oriented segments were ligated into a SacI-cleaved plasmid. Using the methods described, we were able to assemble PCR products into appropriate structures.     

Preoperative biliary drainage, colonisation of bile and postoperative complications in patients with tumours of the pancreatic head: a retrospective analysis of 241 consecutive patients

OBJECTIVE: To analyse the outcome of preoperative biliary drainage in patients being operated on for a tumour in the pancreatic head. DESIGN: Retrospective study. SETTING: University hospital, The Netherlands. SUBJECTS: Consecutive series of 241 patients. MAIN OUTCOME MEASURES: Decline in bilirubin concentrations and bacterial contamination of bile as a result of preoperative drainage. Incidence of postoperative complications in patients who underwent preoperative drainage and those who did not. RESULTS: 184/241 patients underwent preoperative biliary drainage. Endoscopic drainage was the most effective, shown by a median reduction in bilirubin concentrations of 82%, 74%, and 50% after endoscopic drainage (n = 149), papillotomy (n = 25) and external drainage (n = 10), respectively. Bacterial contamination of bile was significantly more common when an endoprosthesis was used, but did not result in a higher rate of infective complications. 163 Whipple's resections, 33 total pancreatectomies, and 45 biliary-enteric bypasses were performed. 137/241 (57%) patients had postoperative complications. There was no significant difference in the incidence of postoperative complications between patients who had preoperative biliary drainage and those who did not (p = 0.4).     

Morphology of oligomers and their network polymers

Summary The morphology of di (4-methacryloxyethylene carbamate toluylen-2-carbamic) esters of oligoethylene glycols (OUM) with different molecular weight, of their network polymers and of model oligomers without methacrylic groups was studied. The supermolecular structure of oligomers is found to depend on molecular weight. Globular structure is typical for low molecular weight oligomers with M_n1200. The structure of crystalline samples with M= 1600–3600 is lamellar. The latter is retained on melting. It is transformed into globular structure at a temperature much higher than the melting point. A correlation exists between the morphology of polymerizable oligomers and their network polymers. The process of network formation from oligomers is extremely complicated. Irrespective of the mechanism it is influenced by MWD, FTD, intermolecular interaction and a number of other factors. The interconnection between the morphology of polymerizable oligomers and the supermolecular structure of their crosslinked polymers has not been sufficiently studied.Information about this connection facilitates phenomenological understanding of the network formation process.Presented at the 7th IUPAC Discussion Conference Polymer Networks, Karlovy Vary, CSSR, September 1980