Structural,mechanical and electrical-contact properties of nanocrystalline-NbC/amorphous-C coatings deposited by magnetron sputtering

Niobium-carbide nanocomposite coatings with a carbon content varying from 43 to 64 at.% were deposited by dual DC magnetron sputtering. X-ray diffraction, x-ray photoelectron spectroscopy and electron microscopy showed that all coatings consisted of nanometer sized NbC grains embedded in a matrix of amorphous carbon. Mechanical properties and electrical resistivity showed a strong dependency on the amount of amorphous carbon (a-C) and NbC grain size in the coating. The highest hardness (23 GPa), elastic modulus (295 GPa) and the lowest resistivity (260 μΩ cm) were measured for the coating with about 15% of a-C phase. Contact resistance measurements using a crossed cylinder set-up showed lowest contact resistance for the coating containing 33% a-C (140 μΩ at a contact force of 100 N), which is comparable to a Ag reference (45 μΩ at a contact force of 100 N). Comparison with TiC-based nanocomposites studied under similar conditions showed that the NbC system has less tendency to form a-C and that lowest contact resistance is obtained at comparable amounts of a-C phase in both material systems (33% for NbC compared to 35% for TiC). With these good electrical contact properties, the NbC nanocomposites can be considered as a potential material for electrical contact applications.     

Tribofilm formation from TiC and nanocomposite TiAlC coatings,studied with focused ion beam and transmission electron microscopy

This work demonstrate how two different carbide coatings respond very differently to tribological stress and their very different ability to provide low friction tribofilms in dry sliding against steel. Both coatings, TiC and TiAlC, were deposited by DC-magnetron sputtering, but while the TiC is a thermodynamically stable coating, the TiAlC is made metastable with the addition of Al, and therefore releases carbon upon tribological testing. Thus, the TiAlC coating is shown to be self-lubricating on the atomic scale which makes very low friction achievable. The primary interest in this study is the differences in the tribofilms formed on the steel balls that have been sliding against the two coatings. Cross-section samples for transmission electron microscopy were extracted from the ball tribofilms using a focused ion beam instrument. X-ray photoelectron spectroscopy and Raman analysis were employed to provide information on the chemical and structural characteristics of the tribofilms. It was shown that tribofilms on steel balls largely inherit the structure and composition that evolve in the coating wear tracks, that the tribofilm microstructure greatly affects the friction level. It was also shown that tribofilm delamination, occurring with tribofilm growth, was initiated in weak ribbon like regions inside the tribofilm.     

Reliability analysis of a sheet metal forming process using Monte Carlo analysis and metamodels

The aim of the present paper is to evaluate the use of linear and quadratic approximating response surfaces as metamodels in a reliability assessment of a sheet metal forming process using the Monte Carlo simulation technique. Monte Carlo simulation was used to determine the probability for springback and thickness variation in a sheet metal part. The conclusions of this study is that Monte Carlo analysis can be used to identify the most important variables and to estimate the range of the studied responses. Linear metamodels can be used to identify the important variables and to give an estimate of the probabilistic response. But quadratic surfaces are required for a more accurate analysis.     

Experimental investigation of surface crack initiation, propagation and tension stiffening in self-compacting steel–fibre-reinforced concrete

To investigate crack initiation and propagation in reinforced, self-compacting, steel?Cfibre-reinforced concrete (SCSFRC) members, tie elements were tested in tension. Strain and surface crack formation were monitored with an optical strain measurement system based on digital image correlation. In addition, to capture the softening behaviour (???Cw) of the material, uni-axial tension testing was performed on SCSFRC cylinders. The results show that, with the optical strain measurement system, it was possible to detect different cracking modes and to follow the crack growth. It was especially of interest to recognize that high fibre amounts tend to change a sudden opening of a crack (as in non-fibrous concrete) into a more stable procedure. It was found that, for a given crack width, the SCSFRC specimens exhibited a noticeably higher tension stiffening than the specimens without fibres. Moreover, at a given load, the crack widths decreased by as much as 65% for the SCSFRC specimens with a nominal fibre content of 1%. For the uni-axial tension tests the results showed that with higher fibre content, for this type of fibre and concrete, both the peak stress and the residual tensile stress were increased. Additionally, it was noted for both specimen types that the scatter in fibre distribution decreased with increasing fibre content.     

Synthesis of C‐Glucosylated Octaketide Anthraquinones in Nicotiana benthamiana by Using a Multispecies‐Based Biosynthetic Pathway

Carminic acid is a C‐glucosylated octaketide anthraquinone and the main constituent of the natural dye carmine (E120), possessing unique coloring, stability, and solubility properties. Despite being used since ancient times, longstanding efforts to elucidate its route of biosynthesis have been unsuccessful. Herein, a novel combination of enzymes derived from a plant (Aloe arborescens, Aa), a bacterium (Streptomyces sp. R1128, St), and an insect (Dactylopius coccus, Dc) that allows for the biosynthesis of the C‐glucosylated anthraquinone, dcII, a precursor for carminic acid, is reported. The pathway, which consists of AaOKS, StZhuI, StZhuJ, and DcUGT2, presents an alternative biosynthetic approach for the production of polyketides by using a type III polyketide synthase (PKS) and tailoring enzymes originating from a type II PKS system. The current study showcases the power of using transient expression in Nicotiana benthamiana for efficient and rapid identification of functional biosynthetic pathways, including both soluble and membrane‐bound enzymes.