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81.
This paper discusses the issue of unbalanced bid prices in unit price highway contracts. It analyzes the reasons contractors unbalance their bids and looks for ways that allow public transportation agencies to discourage this practice. It reviews the results of a study of quantity estimating accuracy of 462 transportation projects in Oklahoma, and finds that one method to reduce unbalanced bid prices is for the agency to ensure that the bid quantities used in the engineer’s estimate are as accurate as possible. Doing so reduces the need for contractors to unbalance to protect fixed costs and target profit on bid items that will underrun the quantity used in the engineer’s estimate. 相似文献
82.
Suzuki C.; Kawano M.; Kashiwagi T.; Arata Y.; Kawasumi T.; Kashiwagi Y. 《Protein engineering, design & selection : PEDS》2000,13(2):73-76
Expression of the SMK1 gene which encodes the yeast killer toxinSMKT is lethal in Saccharomyces cerevisiae. Effects of deletionand site-directed mutagenesis of SMK1 on the lethality and thesecretion of the gene products were examined. Deletion of theinterstitial 相似文献
83.
Fabio Bioletto Martina Bollati Chiara Lopez Stefano Arata Matteo Procopio Federico Ponzetto Ezio Ghigo Mauro Maccario Mirko Parasiliti-Caprino 《International journal of molecular sciences》2022,23(9)
Primary aldosteronism (PA) is a pathological condition characterized by an excessive aldosterone secretion; once thought to be rare, PA is now recognized as the most common cause of secondary hypertension. Its prevalence increases with the severity of hypertension, reaching up to 29.1% in patients with resistant hypertension (RH). Both PA and RH are “high-risk phenotypes”, associated with increased cardiovascular morbidity and mortality compared to non-PA and non-RH patients. Aldosterone excess, as occurs in PA, can contribute to the development of a RH phenotype through several mechanisms. First, inappropriate aldosterone levels with respect to the hydro-electrolytic status of the individual can cause salt retention and volume expansion by inducing sodium and water reabsorption in the kidney. Moreover, a growing body of evidence has highlighted the detrimental consequences of “non-classical” effects of aldosterone in several target tissues. Aldosterone-induced vascular remodeling, sympathetic overactivity, insulin resistance, and adipose tissue dysfunction can further contribute to the worsening of arterial hypertension and to the development of drug-resistance. In addition, the pro-oxidative, pro-fibrotic, and pro-inflammatory effects of aldosterone may aggravate end-organ damage, thereby perpetuating a vicious cycle that eventually leads to a more severe hypertensive phenotype. Finally, neither the pathophysiological mechanisms mediating aldosterone-driven blood pressure rise, nor those mediating aldosterone-driven end-organ damage, are specifically blocked by standard first-line anti-hypertensive drugs, which might further account for the drug-resistant phenotype that frequently characterizes PA patients. 相似文献
84.
Keisuke Nakajima Saori Oka Takashi Tanikawa Yoko Nemoto-Sasaki Naoki Matsumoto Hiroki Ishiguro Yoichiro Arata Takayuki Sugiura Atsushi Yamashita 《International journal of molecular sciences》2022,23(18)
We previously reported that lysophosphatidylinositol (LPI) functions as an endogenous agonist of GPR55, a novel cannabinoid receptor. However, the physiological roles of LPI-GPR55 have not yet been elucidated in detail. In the present study, we found that LPI induced morphological changes in GPR55-expressing HEK293 cells. LPI induced the cell rounding of GPR55-expressing HEK293 cells but not of empty-vector-transfected cells. LPI also induced the activation of small GTP-binding protein RhoA and increased stress fiber formation in GPR55-expressing HEK293 cells. The inhibition of RhoA and Rho kinase ROCK by the C3 exoenzyme and the ROCK inhibitor reduced LPI-induced cell rounding and stress fiber formation. These results clearly indicated that the LPI-induced morphological changes and the assembly of the cytoskeletons were mediated through the GPR55-RhoA-ROCK pathway. 相似文献
85.
Niemeyer Caleb Nasielski Joshua Janovicek Ken Bruulsema Tom Deen Bill 《Nutrient Cycling in Agroecosystems》2021,121(1):115-128
Nutrient Cycling in Agroecosystems - Nitrogen (N) fertilizer decision support systems that rely primarily on corn grain yield often perform poorly because year-to-year variations in net soil N... 相似文献
86.
Yanclo Loriane A. Sigge Gunnar Belay Zinash A. October Feroza Caleb Oluwafemi J. 《Food science and biotechnology》2022,31(3):311-322
Food Science and Biotechnology - Nectarine fruit is highly perishable due to its high moisture content (89%) and susceptibility to decay. Continuous degradation in quality attributes due to... 相似文献
87.
A generalized computational methodology for reduced order acoustic‐structural coupled modeling of the aeroacoustics of a wind turbine blade is presented. This methodology is used to investigate the acoustic pressure distribution in and around airfoils to guide the development of a passive damage detection approach for structural health monitoring of wind turbine blades for the first time. The output of a k ? ε turbulence model computational fluid dynamics simulation is used to calculate simple acoustic sources on the basis of model tuning with published experimental data. The methodology is then applied to a computational case study of a 0.3048‐m chord NACA 0012 airfoil with two internal cavities, each with a microphone placed along the shear web. Five damage locations and four damage sizes are studied and compared with the healthy baseline case for three strategically selected acoustic frequencies: 1, 5, and 10 kHz. In 22 of the 36 cases in which the front cavity is damaged, the front cavity microphone measures an increase in sound pressure level (SPL) above 3 dB, while rear cavity damage only results in six out of 24 cases with a 3‐dB increase in the rear cavity. The 1‐ and 5‐kHz cases show a more consistent increase in SPL than the 10‐kHz case, illustrating the spectral dependency of the model. The case study shows how passive acoustic detection could be used to identify blade damage, while providing a template for application of the methodology to investigate the feasibility of passive detection for any specific turbine blade. 相似文献
88.
Kruse Natalie Hawkins Caleb López Dina L. Johnson Kelly 《Mine Water and the Environment》2019,38(4):718-734
Mine Water and the Environment - The East Branch of Raccoon Creek, Ohio is highly impacted by pre-regulation coal mining and contains 10 steel slag leach beds that passively treat the low pH, and... 相似文献
89.
Sarah Taylor David Baker Philip Owuor John Orchard Caleb Othieno Clifton Gay 《Journal of the science of food and agriculture》1992,58(2):185-191
The plant pigment (carotenoid and chlorophyll) composition of fresh green leaf of tea for a selection of clones was studied in relation to the quality of black tea produced from processing the same green leaf. The plant pigment composition of the green leaf for nine Kenyan clones was monitored by HPLC at 450 nm. Using discriminant analysis it was shown that clones could be distinguished from one another on the basis of their green leaf plant pigment composition. Using step-wise multiple regression analysis, a linear relationship was established between the levels of six green leaf plant pigments peak 5 (neochrome), peak 17 and 18 (isomers of lutein and/or lutein epoxide), peak 23 (chlorophyll b type compound), peak 26 (aurochrome) and peak 28 (β-carotene)) and the quality of the manufactured black tea for the selected clones as assessed by tea tasters' evaluations. The model had an adjusted R-square value of 0.912 and was significant at P≤ 0.05. The value of the relationship as a means of predicting black tea quality is discussed. 相似文献
90.
We investigated prolidase activity in samples derived from wound fluid as well as blister fluid. Prolidase activity was elevated in fluid samples collected from wounds over the levels in sera collected from patients with chronic wounds (P < 0.05). Prolidase activity was also present in samples taken from blister diseases. However, prolidase activity in blister fluid was not higher than that in sera collected from patients with blister diseases. Our results indicate that prolidase may play a role in wound healing. 相似文献