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61.
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SA Klein JM Dobmeyer TS Dobmeyer M Pape OG Ottmann EB Helm D Hoelzer R Rossol 《Canadian Metallurgical Quarterly》1997,11(9):1111-1118
OBJECTIVE: To characterize changes of Th1/Th2 cytokine production by peripheral blood mononuclear cells (PBMC) that occur during the course of HIV infection by cytoplasmic cytokine staining on single cell level. DESIGN AND METHODS: Mitogen-stimulated PBMC from 16 healthy donors, 18 HIV-1-infected individuals without AIDS and 14 patients with AIDS were stained intracellularly with fluorescein-labelled MAb against interleukin (IL)-2, IL-4, IL-10 and interferon (IFN)-gamma. Additionally, co-staining of CD4+ T-cell, CD8+ T-cell, natural killer (NK) cell, B-cell and monocytic markers was performed. Fluorescence staining was analysed by three-colour flow-cytometry. RESULTS: A reduced percentage of IL-2 and IFN-gamma (Th1 type)-producing cells among CD4+ T cells from HIV-1-infected individuals could be demonstrated. There was a continuous decrease of IFN-gamma-producing CD4+ T cells in the course of HIV infection and a dramatic reduction of IL-2-expressing cells among CD4+ T cells in patients with AIDS. In contrast to Th1 cytokines, the frequency of Th2 cytokine expressing cells among CD4+ T cells increased in HIV-infected individuals. The maximum frequency of IL-4-expressing cells among CD4+ T cells was seen in HIV-infected individuals without AIDS, whereas the rate of IL-10-producing cells was highest in patients with AIDS. In HIV-infected individuals no significant proportion of Th0 cells expressing both Th1 and Th2 cytokines was detectable. In CD8+ T cells the percentage of IL-2 was expressing cells decreased continuously accompanied by a strong increase of the frequency of IFN-gamma-producing cells. CONCLUSION: The decreased percentage of cells expressing IL-2 and IFN-gamma in conjunction with an increased proportion of IL-4- and IL-10-producing cells among the CD4+ T cells in HIV-1-infected individuals demonstrate a Th1 to Th2 cytokine shift in the course of HIV infection on a single cell level. There was no evidence of a Th1 to Th0 cytokine shift. In addition to the loss of CD4+ T cells in HIV infection, the qualitative changes of Th1/Th2 cytokine expression may serve as a marker for progressive failure of cell-mediated immunity. 相似文献
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In recent years unmanned vehicles have grown in popularity, with an ever increasing number of applications in industry, the military and research within air, ground and marine domains. In particular, the challenges posed by unmanned marine vehicles in order to increase the level of autonomy include automatic obstacle avoidance and conformance with the Rules of the Road when navigating in the presence of other maritime traffic. The USV Master Plan which has been established for the US Navy outlines a list of objectives for improving autonomy in order to increase mission diversity and reduce the amount of supervisory intervention. This paper addresses the specific development needs based on notable research carried out to date, primarily with regard to navigation, guidance, control and motion planning. The integration of the International Regulations for Avoiding Collisions at Sea within the obstacle avoidance protocols seeks to prevent maritime accidents attributed to human error. The addition of these critical safety measures may be key to a future growth in demand for USVs, as they serve to pave the way for establishing legal policies for unmanned vessels. 相似文献
65.
A Gaussian sum filter (GSF) with component extended Kalman filters (EKF) is proposed as an approach to localizing an autonomous vehicle in an urban environment with limited GPS availability. The GSF uses vehicle‐relative vision‐based measurements of known map features coupled with inertial navigation solutions to accomplish localization in the absence of GPS. The vision‐based measurements have multimodal measurement likelihood functions that are well represented as weighted sums of Gaussian densities. The GSF is used because of its ability to represent the posterior distribution of the vehicle pose with better efficiency (fewer terms, less computational complexity) than a corresponding bootstrap particle filter with various numbers of particles because of the interaction with measurement hypothesis tests. The expectation‐maximization algorithm is used off line to determine the representational efficiency of the particle filter in terms of an effective number of Gaussian densities. In comparison, the GSF, which uses an iterative condensation procedure after each iteration of the filter to maintain real‐time capabilities, is shown through a series of in‐depth empirical studies to more accurately maintain a representation of the posterior distribution than the particle filter using 37 min of recorded data from Cornell University's autonomous vehicle driven in an urban environment, including a 32 min GPS blackout. © 2012 Wiley Periodicals, Inc. 相似文献
66.
Smith PM Campbell CK 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》1989,36(1):10-15
A study of low-loss surface-acoustic-wave (SAW) interdigitated interdigital transducer (IIDT) structures is presented. A model based on mixed scattering matrices was used in the analysis. It is shown that the inherent comb frequency response of the IIDT can be smoothed by slanting the transducers in the structure. Alternatively, the combs can be enhanced to produce a compact low-loss feedback element for potential application in multimode oscillators. Experimental verification of the analysis is provided by a 3:2 slanted-finger IIDT filter with a 10% relative bandwidth of 5.9-dB insertion loss, and a comb filter with 13.4-dB insertion loss and comb spacing of about 3 MHz. The measured responses agree quite well with the theory. 相似文献
67.
Edmonson PJ Smith PM Campbell CK 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》1992,39(5):631-637
The use of surface-transverse-wave (STW) resonator-based oscillators as amplifiers and as carrier recovery elements is discussed. It is demonstrated that these oscillators can amplify phase-shift-keyed signals with very little added noise, while providing a constant output power. Their performance in carrier recovery amplifications is analyzed. Experimental results showing the amplification with more than 80 dB of dynamic range of a 2 Mb/s BPSK signal and the carrier recovery of an 8 Mb/s signal with a 1-GHz STW oscillator are shown. 相似文献
68.
Campbell CK Smith PM Edmonson PJ 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》1992,39(6):768-773
Coupling-of-modes-in-time and coupling-of-modes-in-space analyses are applied to the modeling of a two-port two-pole waveguide-coupled SAW resonator filter. Modeling is applied to a lumped equivalent circuit and a distributed circuit. While the accuracy of the coupling estimation dictates the exact mode-splitting and bandwidth, the ensuing computations can give a good representation of the amplitude and phase response, including the effect of degenerate transverse modes. 相似文献
69.
D. T. Cambell and D. W. Fiske (1959) made the now-obvious statement that measures of the same variable made by different methods should agree (converge) and certainly should agree better than measures of different variables made by those several methods. Almost everyone accepted that statement, yet the multitrait–multimethod matrices published today show little or no improvement over the published examples cited in 1959. The article raised the conceptual problem of how a variable and its measurement should be linked and the methodological problem of clarifying the nature of methods and their effects. Given these unresolved problems, it is perhaps not surprising that the question of the appropriate statistical analysis of these matrices has also no consensual answer. Perhaps methods and traits or contents are so thoroughly intertwined that their interaction cannot be adequately analyzed. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
70.