Extraction of DNA from malaria-infected erythrocytes using isotachophoresis

We demonstrate a technique for purification of nucleic acids from malaria parasites infecting human erythrocytes using isotachophoresis (ITP). We release nucleic acids from malaria-infected erythrocytes by lysing with heat and proteinase K for 10 min and immediately, thereafter, load sample onto a capillary device. We study the effect of temperature on lysis efficiency. We also implement pressure-driven counterflow during ITP extraction to extend focusing time and increase nucleic acid yield. We show that the purified genomic DNA samples are compatible with polymerase chain reaction (PCR) and demonstrate a clinically relevant limit of detection of 0.5 parasites per nanoliter using quantitative PCR.     

The role of familism in stress and coping processes among African American and White dementia caregivers: Effects on mental and physical health.

Objective: To explore how familism, burden, and coping styles mediate the relationships between ethnicity and the mental and physical health of caregivers. Design: A probability sample of 65 White and 95 African Americans respondents caring for an older family member with dementia was used to test hypotheses from a sociocultural stress and coping model using path analysis. Main outcome measures: Measures of caregivers' health included subjective health, self-reported diseases, blood pressure, and heart rate. Mental health measures included self-reported depression and psychological symptoms. Results: Contrary to the hypothesis, familism had an adverse effect on outcomes and was related to low education levels rather than to African American ethnicity. A buffering effect of active coping between being African American and diastolic blood pressure was found even after controlling for levels of education. Conclusions: Findings supported a core stress and coping model in which more behavior problems of care recipients were associated with poorer mental health of caregivers via greater burden and more use of avoidant coping. Results also demonstrate that this core model can be extended to physical health. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Altered specificities of genetically engineered {alpha}1 antitrypsin variants

Seven active site variants of human ₁-antitrypsin (₁AT) wereproduced in Escherichia coli following site-specific mutagenesisof the ₁AT complementary DNA. ₁AT (Ala ³⁵⁸), ₁AT (Ile³⁵⁸ and₁AT (Val³⁵⁸), were efficient inhibitors of both neutrophil andpancreatic elastases, but not of cathepsin G. ₁AT (Ala³⁵⁸, Val³⁵⁸)and ₁AT (Phe³⁵⁸ specifically inhibited pancreatic elastase andcathepsin G respectively. The most potent inhibitor of neutrophilelastase was ₁AT (Leu³⁵⁸), which also proved to be effectiveagainst cathepsin G. The ₁AT (Arg³⁵⁸) variant inactivated thrombinwith kinetics similar to antithrombin III in the presence ofheparin. Electrophoretic analysis showed that SDS-stable highmol. wt complexes were formed between the mutant inhibitorsand the cognate proteases in each case. These data indicatethat effective inhibition occurs when the ₁AT P1 residue (position358) corresponds to the primary specificity of the target protease.Moreover, alteration of the P3 residue (position 356) can furthermodify the reactivity of the inhibitor. Two of the variantshave therapeutic potential: ₁AT (Leu³⁵⁸ may be more useful thanplasma ₁AT in the treatment of destructive lung disorders and₁ (Arg³⁵⁸ could be effective in the control of thrombosis.     

Chemical Thermodynamic Analysis of Silicon Doping in MOCVD of GaAs System

With chemical thermodynamic method,enthalpies,entropies and heat capacities of(SiH_3)_nAsH_(3-n)(1≤n≤3),(CH_3)_mSiH_(4-m)(1≤m≤4)and their radicals were calculated.Homogeneous reactions equilibrium of 65gas phase species in SiH_4(or Si_2H_6)doped MOCVD GaAs by TMG and AsH_3 system was analyzed,the re-lations of gas phase partial pressures with growth temperatures and input partial pressures were calculated.When the gas phase is saturated with a GaAs:Si solid,the gas phase partial pressures and solid phase siliconimpurity(Si_(Ga)-As,Ga-Si_(As),Si_(Ga)-Si_(As))concentrations were calculated under different growth temperatures andinput partial pressures.With the above results,some of the Si doping behavior in MOCVD of GaAs are ex-plained.     

Meaning in the context of stress and coping.

Although theoretical and empirical work on topics related to meaning and meaning making proliferate, careful evaluation and integration of this area have not been carried out. Toward this end, this article has 3 goals: (a) to elaborate the critical dimensions of meaning as it relates to stressful life events and conditions, (b) to extend the transactional model of stress and coping to include these dimensions, and (c) to provide a framework for understanding current research and directions for future research within this extended model. First, the authors present a framework for understanding diverse conceptual and operational definitions of meaning by distinguishing 2 levels of meaning, termed global meaning and situational meaning. Second, the authors use this framework to review and synthesize the literature on the functions of meaning in the coping process and propose a definition of meaning making that highlights the critical role of reappraisal. The authors specify the roles of attributions throughout the coping process and discuss implications for future research. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Sensitivity of vegetation phenology detection to the temporal resolution of satellite data

Vegetation phenology derived from satellite data has increasingly received attention for applications in environmental monitoring and modelling. The accuracy of phenological estimates, however, is unknown at the regional and global level because field validation data are insufficient. To assess the accuracy of satellite‐derived phenology, this study investigates the sensitivity of phenology detection to both the temporal resolution of sampling and the number of consecutive missing values (usually representing cloud cover) in the time series of satellite data. To do this, time series of daily vegetation index data for various ecosystems are modelled and simulated using data from Moderate‐Resolution Imaging Spectroradiometer (MODIS) data. The annual temporal data are then fitted using piecewise logistic functions, which are employed to calculate curvature change rate for detecting phenological transition dates. The results show that vegetation phenology can be estimated with a high precision from time series with temporal resolutions of 6–16 days even if daily data contains some uncertainties. If the temporal resolution is no coarser than 16 days for time series sampled using an average composite, the absolute errors are less than 3 days. On the other hand, the phase shift of temporal sampling is shown to have limited impacts on phenology detection. However, the accuracy of phenology detection may be reduced greatly if missing values in the time series of 16‐day MODIS data occur around the onsets of phenological transition dates. Even so, the probability that the absolute error in phenological estimates is greater than 5 days is less than 4% when only one period is missing in the time series of 16‐day data during vegetation growing seasons; this probability increases to 20% if there are two consecutive missing values.     

An algorithm for the retrieval of 30-m snow-free albedo from Landsat surface reflectance and MODIS BRDF

We present a new methodology to generate 30-m resolution land surface albedo using Landsat surface reflectance and anisotropy information from concurrent MODIS 500-m observations. Albedo information at fine spatial resolution is particularly useful for quantifying climate impacts associated with land use change and ecosystem disturbance. The derived white-sky and black-sky spectral albedos may be used to estimate actual spectral albedos by taking into account the proportion of direct and diffuse solar radiation arriving at the ground. A further spectral-to-broadband conversion based on extensive radiative transfer simulations is applied to produce the broadband albedos at visible, near infrared, and shortwave regimes. The accuracy of this approach has been evaluated using 270 Landsat scenes covering six field stations supported by the SURFace RADiation Budget Network (SURFRAD) and Atmospheric Radiation Measurement Southern Great Plains (ARM/SGP) network. Comparison with field measurements shows that Landsat 30-m snow-free shortwave albedos from all seasons generally achieve an absolute accuracy of ±0.02-0.05 for these validation sites during available clear days in 2003-2005, with a root mean square error less than 0.03 and a bias less than 0.02. This level of accuracy has been regarded as sufficient for driving global and regional climate models. The Landsat-based retrievals have also been compared to the operational 16-day MODIS albedo produced every 8-days from MODIS on Terra and Aqua (MCD43A). The Landsat albedo provides more detailed landscape texture, and achieves better agreement (correlation and dynamic range) with in-situ data at the validation stations, particularly when the stations include a heterogeneous mix of surface covers.