Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene in E. coli

Ethylene is an essential platform chemical with a conjugated double bond, which can produce many secondary chemical products through copolymerisation. At present, ethylene production is mainly from petroleum fractionation and cracking, which are unsustainable in the long term, and harmful to our environment. Therefore, a hot research field is seeking a cleaner method for ethylene production. Based on the model ethylene-forming enzyme (Efe) {"type":"entrez-protein","attrs":{"text":"AAD16440.1","term_id":"4323597","term_text":"AAD16440.1"}}AAD16440.1 (6vp4.1.A) from Pseudomonas syringae pv. phaseolicol, we evaluated five putative Efe protein sequences using the data derived from phylogenetic analyses and the conservation of their catalytic structures. Then, pBAD expression frameworks were constructed, and relevant enzymes were expressed in E. coli BL21. Finally, enzymatic activity in vitro and in vivo was detected to demonstrate their catalytic activity. Our results show that the activity in vitro measured by the conversion of α-ketoglutarate was from 0.21–0.72 μmol ethylene/mg/min, which varied across the temperatures. In cells, the activity of the new Efes was 12.28–147.43 μmol/gDCW/h (DCW, dry cellular weight). Both results prove that all the five putative Efes could produce ethylene.     

Exploring the Effects of the Interaction of Carbon and MoS2 Catalyst on CO2 Hydrogenation to Methanol

Hydrogenation of CO₂ to form methanol utilizing green hydrogen is a promising route to realizing carbon neutrality. However, the development of catalyst with high activity and selectivity to methanol from the CO₂ hydrogenation is still a challenge due to the chemical inertness of CO₂ and its characteristics of multi-path conversion. Herein, a series of highly active carbon-confining molybdenum sulfide (MoS₂@C) catalysts were prepared by the in-situ pyrolysis method. In comparison with the bulk MoS₂ and MoS₂/C, the stronger interaction between MoS₂ and the carbon layer was clearly generated. Under the optimized reaction conditions, MoS₂@C showed better catalytic performance and long-term stability. The MoS₂@C catalyst could sustain around 32.4% conversion of CO₂ with 94.8% selectivity of MeOH for at least 150 h.     

初中物理习题解决的数据驱动加工方式

通过分析认知加工理论的发展历程,认为物理习题解决属于数据驱动加工,而物理问题解决属于概念驱动加工.结合初中物理习题的解决,阐述了数据驱动加工的原理、分类及解题过程.     

华蟾素注射液对人肝癌HepG-2细胞增殖及周期的影响

Objective: The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and cell cycle of human hepatoma HepG-2 cells. Methods: Cell proliferation was assessed by MTT assay, cell cycle distributionwas detected by the flow cytometry (FCM). The expression of Cyclin A, CDK2 mRNA levels were examined by RT-PCR.Quantitative colorimetric assay was used to analyze Cyclin NCDK2 activity in HepG-2 cells. Results: Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose- and time-dependent ways; FCM analysis showed Cinobufacini injection induced cell cycle arrest at S phase; RT-PCR assay showed Cinobufacini injection down-regulated Cyclin A, CDK2expression at mRNA levels; Quantitative colorimetric assay showed Cinobufacini injection deceased Cyclin A/CDK2 activity in HepG-2 cells. Conclusion: Cinobufacini injection can inhibit human hepatoma HepG-2 cells growth, induce cell apoptosis and induce cell cycle arrest at S phase, the mechanism of which might be partly related to the down-regulation of Cyclin A,CDK2 mRNA expression and inhibition of Cyclin A/CDK2 activity.     

Structural design and mechanical performance test of densely drilled glass fiber reinforced plastics pipelines

Densely drilled glass fiber reinforced plastic (GFRP) pipelines are thought to be of prosperous future in sand control in oilfield. However, due to the fact that the mechanical properties of the perforated GFRP pipelines are always lowered remarkably, some structural design work must be done. Several key factors, including the winding angle of the glass fibers, the mechanical properties of the matrix resin were considered carefully in advance. The tensile strength, compressive strength and the screw-crushing properties of the GFRP pipelines were measured subsequently. Most of the test results were coincided with what had been expected during structural design. The winding angle was proved to be an important factor in improving the mechanical performance of perforated GFRP pipelines. Mechanical property of the matrix resin was another important factor in determining the performance of the GFRP pipelines. The effect of the hole-size and distribution on the mechanical performance of the heavily perforated GFRP pipelines always depended on the winding angle and matrix resin. In the screw-crushing test, the GFRP pipelines showed to be easily destroyed by the slowly rotating drill head.     

Effects of phospholipid hydroperoxide glutathione peroxidase mRNA expression on meat quality of <Emphasis Type=Italic>M. longissimus dorsi</Emphasis> in pigs

Antioxidant enzymes protect muscle tissues against oxidation from reactive oxygen species and increase the stability of the muscle. Phospholipid hydroperoxide glutathione peroxidase (GPx4) is an important antioxidant enzyme in muscle tissue. So the aim of this study was to identify the effects of mRNA expression level of GPx4 gene on meat quality properties in Longissimus dorsi of three pig breeds. Total 36 pigs (12 pigs for each breed) were used to measure the meat quality properties and to analyze the expression levels of GPx4 gene by quantitative real-time PCR. The results showed that meat quality, GPx4 activity, and GPx4 mRNA levels were affected by pig breeds. There were the lowest expressible moisture, drip loss, and cooking loss in Laiwu Pig among the three breeds, and also there were the highest GPx4 activity and the expression levels of GPx4 gene in Laiwu Pig compared with the other two pig breeds. Analysis of meat samples divergent for water-holding capacity identified highly negative associations between expressions levels of GPx4 gene and expressible moisture, drip loss and cooking loss. These results suggest that the higher mRNA expression level of GPx4 gene up-regulate the water-holding capacity and the activity of GPx4, and the expression of GPx4 gene plays an important role in physiological changes related to the meat quality properties and the mechanisms of the antioxidant ion of meat.     

基于深度学习的采摘机器人目标识别定位算法

为了提升采摘机器人对果实的识别准确率以及定位定精度,提出一种基于深度学习Faster-RCNN框架的采摘机器人目标识别和定位算法.首先采用卷积神经网络VGG16模型提取输入图像的特性信息,并利用区域提议网络RPN生成含有目标的候选框,通过引入自适应候选框数的方法有效提升了算法性能,然后利用多任务损失函数对目标进行分类识...     

A model relating tool torque and its associated power and specific energy to rotation and forward speeds during friction stir welding/processing

Torque (M) is a very important quantity in the friction stir welding (FSW) process but a satisfactory model relating M to the two major FSW parameters, rotation speed (ω) and forward speed (v), has not been well developed. In this work, a better model of the form, M=f(ω, v), was sought. Experimentally, FSW of an aluminium alloy was conducted over a wide range of ω and v values and M was measured. An exponential decay function has been found to fit all the M versus ω data well and is meaningful for any ω value. The effect of v on M can approximately be accounted for through linearly relating the model parameters to v. The model allows for a detailed evaluation of the sensitivity of M to changes in ω and v. The model decay and the pre-exponential parameters need to be adjusted to make predictions for different aluminium alloys in the low ω range. Both the model and experimental data demonstrate a diminishing alloy effect on material flow resistance as ω increases. Furthermore, models of tool power (P_Tool) and specific energy (E_s) can be derived from the model for M and give predictions that are also meaningful over the entire range of ω values. Finally, the dependences of P_Tool and E_s on ω and v and the relationship between E_s and the processing zone temperature will be discussed.     

国内外己内酰胺的供需现状及发展前景

分析了国内外己内酰胺的生产消费现状及发展前景,提出了发展我国己内酰胺生产的建议。