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61.
A simple method has been devised for immobilization of acetylcholinesterase (AChE) covalent bonding to a multiwall carbon
nanotube (MWNT)-cross-linked cellulose acetate composite on a screen-printed carbon electrode (SPCE) and a sensitive and disposable
amperometric sensor for rapid determination of carbaryl pesticide is proposed. The immobilized enzyme was preserved on this
film because of the excellent biocompatibility and non-toxicity of cellulose acetate. Based on the inherent conductive properties
of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl.
MWNT improved the interface enzymatic hydrolysis reaction and increased the amperometric response of the sensor. Under optimum
conditions, the inhibition of carbaryl on AChE increased linearly with the increasing concentration of carbaryl in two ranges,
from 0.01 to 0.5 μg mL−1 and from 2 to 20 μg mL−1, with the correlation coefficients of 0.9985 and 0.9977, respectively. The detection limit was 0.004 μg mL−1 taken as the concentration equivalent to 10% decrease in signal. The sensor showed acceptable stability, accuracy and could
be fabricated in batches, thus it is economic and portable. This type of disposable enzyme-based amperometric sensor has extensive
application potential in environmental monitoring of pesticides. 相似文献
62.
一个通用代理计费系统开发框架的设计与实现 总被引:4,自引:4,他引:0
用户在不同的网络环境中选择不同的代理软件实现上网和用户管理,这些代理软件既有共性又各具特色,为减少开发不同代理计费软件的工作量,提高开发效率,加快开发周期,提出利用软件重用的思想,将代理软件的差异集中到少数几个类的实现上,设计并实现一个通用代理计费系统开发框架,基于此框架利用相对简单的步骤开发了基于SQUID的代理计费系统,验证了框架的实用性和可行性,达到了设计目标。 相似文献
63.
64.
Three new polymeric aromatic lithium sulfonylimides were used as salts in high molecular weight poly(ethylene oxide) (PEO) electrolytes. Their conductivity and electrochemical stability behaviors were investigated. The electrolytes with lithium poly[4,4′‐(hexafluoroisopropylidene)diphenoxy]sulfonylimide (LiPHFIPSI) showed a better conductive performance compared with the other two lithium salts. The best conductivity was obtained for PEO/LiPHFIPSI EO/Li = 16 (1.90 × 10?4 S/cm at 60°C). Thermal analysis indicated that the salts effectively decreased the crystallinity of PEO. Moreover, the electrolytes also had good electrochemical stability and their oxidative potential was to 5.5 V versus Li/Li+. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 85: 1802–1805, 2002 相似文献
65.
66.
BACKGROUND: Defects in the skull have presented difficult reconstructive problems. Recently glass-ionomer cement and preformed. implants have been used to repair bony defects in the skull base and in cranio-facial surgery. Three patients are reported to illustrate possible applications for this new material. METHODS: Ionocap cement and Ionoroc-skull standardized implants have been used. The cement is available as a two component blister pack. When mixed and blended a gel is produced which can be worked for approximately 5 min before hardening. It can then be contoured and drilled to the required shape. The resultant material is biocompatible and biostable, is non-toxic and permanently bonds to bone with no increase in temperature or shrinkage. RESULTS: The material has been used to fill the temporal defect left by transplantation of the temporalis muscle for oral reconstruction, the anterior and lateral walls of the maxilla and following craniofacial resection, the posterior wall of the frontal sinus and anterior skull base. CONCLUSIONS: Three patients with different reconstructive problems have been presented to illustrate the use of an alloplastic material that has a tensile strength similar to bone and firmly adheres to it. The material was found to be safe and easy to use. All three patients have now been followed for 12 months with no adverse affects. 相似文献
67.
In a complex social, political, economic, technological, and/or environmental context, corporate, military, government, and other organizations are often faced with collective decision-making situations. The rationale for group decision exercises is that the judgment of many will usually prove superior to the judgment of one. However, it has been shown that collective decision exercises are often highly dependent on matters of perspective, values and opinion, all of which - being essentially subjective in nature - are beyond the reach of existing formal decision technology. Furthermore, it can be expected that many collective decision exercises - particularly those of strategic import - will not lend themselves to the quantitative analysis instruments that have long dominated the management and decision science repertoire. This does not, however, mean that they must remain entirely and forever outside the bounds of scientific rationality. The ordering protocols which are currently available do not have enough technical mechanics to be relied upon to bring us to any satisfying resolution of a priori disputation. Hence, we propose an a priori ordering reference model that might support consensus-building in a multiple stakeholders context. In order to detect whether the subjective arguments are products of proper reasons or merely instances of raw rhetoric and to suggest how any logical or syntactical flaws might best be repaired, we propose logical ordering facilities and protocols for integrating procedural and instrumental provisions for a group-decision process with two lines of technical innovation: the superimpositional ordering function and the logical ordering support facilities. 相似文献
68.
介绍了高温超导红外探测器电极材料的选择和制备。选用先蒸、后蒸金,再合金化的电极工艺。芯片装配成器件后,器件性能稳定,膜层牢固,接触电阻小。 相似文献
69.
本文介绍了ConcurrentC语言在一分布式系统上的实现。该实现使用了不同于预编译法的编译器的构造方法,同时将Concurrent C语言中的进程实现为UNIX进程中的用户级线索,是ConcurrentC语言在分布式系统上的一种高效实现。 相似文献
70.
In this paper,we address the need for better hands-on learning materials in computing education and the need for integrating mobile computing education into computing curricular.We propose an innovative learning approach that uses state-of-the-art mobile computing technologies and devices to design a highly accessible and real-world relevant hands-on labware for computing education.The labware employs a modular design such that the learning materials can both be combined together into a single course of mobile program development and be integrated into the related computing courses as building blocks.The labware will help students understand the concepts,improve their problem solving skills,and prepare them for the mobile computing industrial workforce.The pilot modules of the labware have been presented to undergraduate students and have received positive feedbacks. 相似文献