Fine Mapping and Functional Analysis of Major Regulatory Genes of Soluble Solids Content in Wax Gourd (Benincasa hispida)

Soluble solids content (SSC) is an important quality trait of wax gourd, but reports about its regulatory genes are scarce. In this study, the SSC regulatory gene BhSSC2.1 in wax gourd was mined via quantitative trait locus (QTL) mapping based on high-density genetic mapping containing 12 linkage groups (LG) and bulked segregant analysis (BSA)-seq. QTL mapping and BSA-seq revealed for the first time that the SSC QTL (107.658–108.176 cM) of wax gourd was on Chr2 (LG2). The interpretable phenotypic variation rate and maximum LOD were 16.033% and 6.454, respectively. The QTL interval contained 13 genes. Real-time fluorescence quantitative expression analysis, functional annotation, and sequence analysis suggested that Bch02G016960, named BhSSC2.1, was a candidate regulatory gene of the SSC in wax gourd. Functional annotation of this gene showed that it codes for a NADP-dependent malic enzyme. According to BhSSC2.1 sequence variation, an InDel marker was developed for molecular marker-assisted breeding of wax gourd. This study will lay the foundation for future studies regarding breeding and understanding genetic mechanisms of wax gourd.     

Ultra-thin Body Buried In0.35Ga0.65As Channel MOSFETs with Extremely Low Off-current on Si Substrates

In this paper, we investigated the electrical properties of the Metal-oxide-semiconductor gate stack of Ti/Al2O3/InP under different annealing conditions. A min...     

车灯座注塑模具设计

通过对车灯座注塑模具的设计,阐述了模具浇注系统的设计方法,旨在降低模具的复杂程度和制造成本;详细介绍了组合式型腔、型芯的结构设计及模具的制造方法。     

Local multi-model integrated soft sensor based on just-in-time learning for mechanical properties of hot strip mill process

The mechanical properties of hot rolled strip are the key index of product quality,and the soft sensing of them is an important decision basis for the control a...     

Al4B2O9纳米棒改性制备优异综合性能的固体聚合物电解质

随着储能设备和电力驱动产品的激增,特别是电动汽车的大规模推广应用,全固态电池被认为是最有可能解决电动设备日益严峻的安全问题和高能量密度需求的策略之一.本文报道了一种Al4B2O9纳米棒改性的聚环氧乙烷(PEO)基固体聚合物电解质(ASPE),其具有高离子电导率、宽电化学窗口、良好的机械性能和阻燃性能.具体来说,因为Al...     

SVD based scale transform invariant observable degree for LTI system

Dear editor, The basic concept of observability is used to express the possibility to recover the state from measurements in modern control theory [1-3].It is a...     

动目标显示雷达模拟器地物回波单元的设计和实现

在分析了地物杂波回波特性的基础上,首先设计了动目标显示雷达模拟器的地物回波形成单元的组成方框图.结合方框图,得出了形成地物回波的基本要素:同步计数电路、计数比较电路、多普勒调制电路.最后结合雷达自身的特点,设计了动目标显示雷达模拟训练器地物回波形成单元的基本组成电路,并将其与雷达联机,取得良好的效果.     

Simulation annealing diagnosis algorithm method for optimized forecast of the dynamic response of floating offshore wind turbines

Design of floating offshore wind turbines(FOWTs)needs reliable and innovative technologies to overcome the challenges on how to better predict the dynamic respo...     

Improving the precision of optical metrology by detecting fewer photons with biased weak measurement

In optical metrological protocols to measure physical quantities, it is, in principle, always beneficial to increase photon number n to improve measurement prec...     

Biological analysis of woodpecker’s brain after impact experiments

Woodpeckers can withstand a fierce impact during pecking. Previous studies have focused on the biomechanical analysis of the pecking process, the properties of the beak and hyoid bone of woodpecker; however, the biological characteristics of the woodpecker brain are also important in resisting impact injuries. We employed impact experiments and biological analysis in normal and injured brains to reveal the impact-resistant biological characteristics of woodpecker brains, as well as the impact energy's biological effects on the woodpecker brain. The hoopoe, which has a similar size but only a slight pecking behavior, was selected as the control group to compare brain morphology and neuronal cells differences in normal brains between woodpecker and hoopoe by sectioning and staining. A loading device was designed to conduct a quantifiable impact energy to the woodpeckers' head. Four groups of woodpeckers were impacted with the same energy on the forehead, beak, tempus and occiput,respectively. Biological changes in the injured brains were evaluated by Nissl staining and enzyme-linked immunosorbent assay.The results showed that:(1) woodpeckers had a larger cerebellum and a higher density of Nissl bodies than hoopoe;(2) Nissl apoptosis appeared in the brain samples after the forehead and the occiput impact experiments, but no obvious Nissl body apoptosis was observed after impact on the tempus and the beak;(3) β-amyloid protein accumulated in the normal status woodpecker brain. This study reveals that: woodpecker brain morphology is well-adapted to impact, woodpecker heads display location-dependent protective performance, with beak and tempus regions having a better protective performance than the forehead and occiput, Nissl apoptosis appears in injured woodpecker brains, and that the accumulation of β-amyloid protein does not show a direct relationship with the injury state of woodpecker's brain tissue in our study.