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The progression of anchorage-dependent epithelial cells to anchorage-independent growth represents a critical hallmark of malignant transformation. Using an in vitro model of human papillomavirus (HPV)-induced transformation, we previously showed that acquisition of anchorage-independent growth is associated with marked (epi)genetic changes, including altered expression of microRNAs. However, the laborious nature of the conventional growth method in soft agar to measure this phenotype hampers a high-throughput analysis. We developed alternative functional screening methods using 96- and 384-well ultra-low attachment plates to systematically investigate microRNAs regulating anchorage-independent growth. SiHa cervical cancer cells were transfected with a microRNA mimic library (n = 2019) and evaluated for cell viability. We identified 84 microRNAs that consistently suppressed growth in three independent experiments. Further validation in three cell lines and comparison of growth in adherent and ultra-low attachment plates yielded 40 microRNAs that specifically reduced anchorage-independent growth. In conclusion, ultra-low attachment plates are a promising alternative for soft-agar assays to study anchorage-independent growth and are suitable for high-throughput functional screening. Anchorage independence suppressing microRNAs identified through our screen were successfully validated in three cell lines. These microRNAs may provide specific biomarkers for detecting and treating HPV-induced precancerous lesions progressing to invasive cancer, the most critical stage during cervical cancer development.  相似文献   
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Magnesium powders are used widely in reactive and energetic composite materials, such as pyrotechnic formulations, solid fuels and explosives, as a vital ignition source. However, magnesium powders are highly reactive with atmospheric oxygen and moisture, forming the inert magnesium oxide and hydroxide. These reactions limit their shelf life, decrease their potency and affects the overall stability of these materials. In order to increase their long-term stability, protective magnesium coatings are desired. In this work, a method for coating magnesium powders using tetramethylcyclotetrasiloxane (D4H) through a vapor-solid interface reaction is described. This reaction requires no solvents, little material, and does not produce any corrosive byproducts. Coated powders demonstrate increased stability when exposed to elevated temperatures and high humidity conditions, with no oxidation of the magnesium observable. Despite improved stability, the coatings do not negatively impact ignition temperature and dynamics for magnesium powders.  相似文献   
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We have developed an x-ray beam-position monitor for detecting the radiation properties of an x-ray free electron laser (FEL). It is composed of four PIN photodiodes that detect backscattered x-rays from a semitransparent diamond film placed in the beam path. The signal intensities from the photodiodes are used to compute the beam intensity and position. A proof-of-principle experiment at a synchrotron light source revealed that the error in the beam position is reduced to below 7 μm by using a nanocrystal diamond film prepared by plasma-enhanced chemical vapor deposition. Owing to high dose tolerance and transparency of the diamond film, the monitor is suitable for routine diagnostics of extremely intense x-ray pulses from the FEL.  相似文献   
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Freeze casting, also known as ice templating, is a particularly versatile technique that has been applied extensively for the fabrication of well-controlled biomimetic porous materials based on ceramics, metals, polymers, biomacromolecules, and carbon nanomaterials, endowing them with novel properties and broadening their applicability. The principles of different directional freeze-casting processes are described and the relationships between processing and structure are examined. Recent progress in freeze-casting assisted assembly of low dimensional building blocks, including graphene and carbon nanotubes, into tailored micro- and macrostructures is then summarized. Emerging trends relating to novel materials as building blocks and novel freeze-cast geometries—beads, fibers, films, complex macrostructures, and nacre-mimetic composites—are presented. Thereafter, the means by which aligned porous structures and nacre mimetic materials obtainable through recently developed freeze-casting techniques and low-dimensional building blocks can facilitate material functionality across multiple fields of application, including energy storage and conversion, environmental remediation, thermal management, and smart materials, are discussed.  相似文献   
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The Thyroid hormone Receptor Interacting Protein 12 (TRIP12) protein belongs to the 28-member Homologous to the E6-AP C-Terminus (HECT) E3 ubiquitin ligase family. First described as an interactor of the thyroid hormone receptor, TRIP12’s biological importance was revealed by the embryonic lethality of a murine model bearing an inactivating mutation in the TRIP12 gene. Further studies showed the participation of TRIP12 in the regulation of major biological processes such as cell cycle progression, DNA damage repair, chromatin remodeling, and cell differentiation by an ubiquitination-mediated degradation of key protein substrates. Moreover, alterations of TRIP12 expression have been reported in cancers that can serve as predictive markers of therapeutic response. The TRIP12 gene is also referenced as a causative gene associated to intellectual disorders such as Clark–Baraitser syndrome and is clearly implicated in Autism Spectrum Disorder. The aim of the review is to provide an exhaustive and integrated overview of the different aspects of TRIP12 ranging from its regulation, molecular functions and physio-pathological implications.  相似文献   
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Interactive Evolutionary Computation (IEC) creates the intriguing possibility that a large variety of useful content can be produced quickly and easily for practical computer graphics and gaming applications. To show that IEC can produce such content, this paper applies IEC to particle system effects, which are the de facto method in computer graphics for generating fire, smoke, explosions, electricity, water, and many other special effects. While particle systems are capable of producing a broad array of effects, they require substantial mathematical and programming knowledge to produce. Therefore, efficient particle system generation tools are required for content developers to produce special effects in a timely manner. This paper details the design, representation, and animation of particle systems via two IEC tools called NEAT Particles and NEAT Projectiles. Both tools evolve artificial neural networks (ANN) with the NeuroEvolution of Augmenting Topologies (NEAT) method to control the behavior of particles. NEAT Particles evolves general-purpose particle effects, whereas NEAT Projectiles specializes in evolving particle weapon effects for video games. The primary advantage of this NEAT-based IEC approach is to decouple the creation of new effects from mathematics and programming, enabling content developers without programming knowledge to produce complex effects. Furthermore, it allows content designers to produce a broader range of effects than typical development tools. Finally, it acts as a concept generator, allowing content creators to interactively and efficiently explore the space of possible effects. Both NEAT Particles and NEAT Projectiles demonstrate how IEC can evolve useful content for graphical media and games, and are together a step toward the larger goal of automated content generation.  相似文献   
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