Different susceptibility to phospholipase A2 treatment of the fluorescence intensity changes in the vicinity of Cys-964 and Lys-501 in the alpha-chain of probe-labeled Na+,K(+)-ATPase

Phospholipase A2 [EC 3.1.1.4] treatment of pig kidney Na+,K(+)-ATPase [EC 3.6.1.3] labeled with fluorescence probes at the alpha-chain reduced the extent of the fluorescence intensity change of an N-[p-(2-benzimidazolyl)phenyl]maleimide (BIPM) probe at Cys-964 to below one-third of the control level accompanying the accumulation of phosphoenzymes. However, it only induced a slight decrease in that of a fluorescence isothiocyanate (FITC) probe at Lys-501 with a large decrease in the rate of change. The addition of phosphatidylserine (PS) or phosphatidylinositol (PI) to the phospholipase-treated BIPM-FITC-labeled enzyme increased the rate of the FITC fluorescence change. Phospholipase treatment of the BIPM-enzyme greatly reduced the Na+,K(+)-ATPase activity. The addition of PS or PI to the treated enzyme induced reactivation. These data and others suggest that Cys-964 and Glu-953 (Rb+ protectable dicyclohexyl carbodiimide binding site) are located in the vicinity of the surface area of the enzyme where hydrocarbon chains of phospholipids are present, and conserved H-bonding amino acids, Thr-955 and Ser-962, are located rather near the center of a domain forming a cation binding route or cage with other hydrophobic transmembrane segments. These data may indicate that the interaction between the BIPM probe and the hydrocarbon chains of phospholipids changes in such a way as to sense the change in the binding state of various ligands accompanying the sequential appearance of reaction intermediates of the enzyme.     

区域内旱情监测站点的布设研究

本文对北京通州辖区范围内组织的大规模的土壤墒情调查数据进行总结。基于地理统计、空间分析以及统计学知识，分析了北京通州旱情分布形势。运用数值分类学的知识对测量数据进行了聚类分析，同时用DB(DaviesBouldin)指标准则研究了区域内合理布设旱情监测站的数量及位置，为建立旱情监测站点提供了理论依据。     

多属性神经网络地震反演在NB油田水平井钻探中的应用

NB油田位于渤海海域,为新近系河流相稠油油田,储层横向分布不稳定,砂体厚度薄、连通性较差,油水关系复杂,开发设计部署了大量水平井。选择神经网络反演方法,建立地震属性与储层参数的非线性关系,进而根据其关系反演得到储层参数数据体,利用该数据体沿层切片提取储层预测信息,研究储层发育规律,指导该油田水平井的部署和钻探,取得了较好的效果,降低了钻探风险。用神经网络地震反演方法进行储层预测研究,指导水平井钻探,在渤海海域尚属首次。     

国家战略发展新能源股价长期走牛可期待

严峻的能源短缺、居高不下的原油价格、日益严重的环境问题已经成为制约中国经济、社会发展的瓶颈。改变现有的以化石能源为主的能源结构,     

先进电子化政府基本模式及实现

电子化政府是运用信息技术创新政府服务的整合概念。建构电子政府已经成为世界范围内政府再造的新趋势。这里讨论了电子化政府基本模式及规划的内容和特点,并具体提出实现的模式、技术路径及方法。     

基于多信道预约可冲突避免的多址接入协议

本文为多跳分布多无线网络提出了一套灵活而有效的自适应获取冲突避免（AACA）的多址接入协议。在该协议中，各节点竞争公共信道，利用RTS/CTS对话形式来预约各业务信道，成功预约后的分组传输不会受到其他节点的干扰。它使用任意确定数目信道，以异步方式工作，并且使得各节点利用半双工无线电台就可以灵活、简便地实现资源预约。分析和比较结果说明，所提出的多信道预约协议可以有效地解决隐藏终端、暴露终端以及侵入终端的问题。     

测量输入光窗台阶指标的指示表的选择

阐述了对近贴聚焦型微通道板像增强管中输入光窗台阶高度和平整度指标进行测量时，所用指示仪表的选择依据，并详细分析了指示表在检定过程中的误差来源，进一步计算出其合成标准不确定度和扩展不确定度，从而选定可用于测试的指标表。     

基于图像的虚拟环境建模技术

综述了基于图像的虚拟现实环境的建模技术，并给出了各种模型表示的理论依据和建模过程的定义。把环境表示分为四类：全景图，光场数据库，深度图像和多投影中心图。全景图的生成包括基于图像相关性和基于图像整合变换的算法。光场数据库的分类主要依据全光函数。同时论述了在各种表示下的采样和建模技术。最后讨论了当前建模技术研究的热点及所需要解决的问题。     

Application of Code Approach for Emergency Evacuation in a Rail Station

This paper presents an emergency evacuation analysis during a mid-platform train fire in an underground rail station. A time-based evacuation scenario is developed taking into account the stairway inaccessibility due to smoke blockage. The calculation approach in NFPA 130 is then applied to a parametric study of this time-based scenario and its reliability is examined using a micro-simulation model. Both methods give consistent predictions of the exiting times. The results demonstrate that with the division of the station into two zones, the exiting time could not meet the code requirements, whereas it does meet the required exiting time without the division. Such division is due to smoke blockage and creates an uneven ratio of the occupant load to the available exiting capacity. This shows that appropriate consideration of the fire and smoke effects is important for station egress evaluation. Other issues such as the fire growth rate and the pre-evacuation time are also discussed.     

Y-Ba-Cu-O超导涂层的研制

用等离子喷涂工艺制备了 Y-Ba-Cu-O 高临界温度超导涂层,涂层的初始转变温度为101K、零电阻温度为86K。电阻率与磁化率的测量结果表明这种超导涂层具有与烧结超导块体相类似的性能,X 光衍射谱显示出涂层主要由 YBa_2Cu_3O_(?)单相化合物组成。