The survival-growth capacity of Salmonella populations on tomato epidermis labeled by a natural-light labeling system was investigated after persistent fears of such marks serving as possible entryways for the pathogenic organisms, alone and in the presence of Pectobacterium carotovorum subsp. carotovorum, a soft-rot organism. Different treatments involving natural-light labeling, fruit waxing, and a five-strain cocktail of Salmonella were applied to mature green tomato surfaces in different sequences prior to storage at 4, 12, or 25°C. Fruit was sampled every 3 days, and Salmonella was enumerated from all treatments and unlabeled fruit, which served as controls. There were no significant differences between treatments or between treatments and controls throughout. The results indicate that the cuticle and epidermal interruptions caused by natural-light labeling do not facilitate the penetration and colonization of the tomato pericarp. In a separate set of experiments, the capacity of Salmonella to penetrate tomato in the presence of a potential synergism with P. carotovorum subsp. carotovorum was investigated. The addition of P. carotovorum at higher, lower, or equal population densities to Salmonella did not significantly alter the behavior of Salmonella on tomatoes stored at 25°C, regardless of natural-light labeling. The inability of P. carotovorum and Salmonella to colonize natural-light-etched surfaces of tomato fruit indicates that the use of this technology does not adversely compromise the surface of tomatoes. 相似文献
The metabolic alterations of Brassica rapa (L.) leaves attacked by larvae of the specialist Plutella xylostella L. (Lepidoptera: Yponomeutidae) and the generalist Spodoptera exigua Hubner (Lepidoptera: Noctuidae) were investigated with nuclear magnetic resonance (NMR) spectroscopy, followed by a multivariate data analysis. The principal component analysis (PCA) of 1H NMR spectra showed that metabolic changes in B. rapa leaves induced by the 2nd and the 4th instars were different from each other. However, the congestion of the one-dimensional 1H NMR spectrum made it difficult to identify discriminating metabolites. To overcome the spectral complexity, several two-dimensional NMR techniques were applied. Of those evaluated, J-resolved spectroscopy, which affords an additional coupling constant, provided a wide range of structure information on differentiating the metabolites. Based on the J-resolved spectra combined with PCA, the major signals contributing to the discrimination were alanine, threonine, glucose, sucrose, feruloyl malate, sinapoyl malate, and gluconapin. 相似文献
Thin-film Bi2Te3- and Sb2Te3-based superlattice (SL) thermoelectric (TE) devices are an enabling technology for high-power and low-temperature applications,
which include low-noise amplifier cooling, electronics hot-spot cooling, radio frequency (RF) amplifier thermal management,
and direct sensor cooling. Bulk TE devices, which can pump heat loads on the order of 10 W/cm2, are not suitable in these applications due to their large size and low heat pumping capacity. Recently, we have demonstrated
an external maximum temperature difference, ΔTmax, as high as 58 K in an SL thin-film p–n couple. This state-of-the-art couple exhibited a cold-side minimum temperature, Tcmin, of −30.9°C. We regularly attain ΔTmax values in excess of 53 K, in spite of the many significant electrical and thermal parasitics that are unique to thin-film
devices. These measurements do not use any complex thermal management at the heat sink to remove the heat flux from the TE
device’s hot side. We describe here multistage SL cooling technologies currently being developed at RTI that can provide useful
microcooling cold-side temperatures of 200 K. This effort includes a three-stage module employing independently powered stages
which produced a ΔTmax of 101.6 K with a Tcmin of −75°C, as well as a novel two-wire three-stage SL cascade which demonstrated a Tcmin of −46°C and a ΔTmax of nearly 74 K. These RTI modules are only 2.5 mm thick, significantly thinner than a similar commercial three-stage module
(5.3 mm thick) that produces a ΔTmax of 96 K. In addition, TE coolers fabricated from these thin-film SL materials perform significantly better than the extrapolated
performance of similar thickness bulk alloy materials. 相似文献
Light up galectin: Photoprobes based on thiodigalactoside were prepared for galectin‐3, a lectin linked to cancer. The probes contained either benzophenone or acetophenone moieties as the photolabel for covalent attachment to the protein. One particular probe labeled galectin‐3 selectively, even in the presence of cell lysate.
Hydroxyapatite (HA) nanoparticles (NPs) doped with different radioisotopes for use as theranostic systems play an important role in scientific research nowadays due to their ability to simultaneously act in the treatment and diagnosis of various types of cancers. In this work, we describe the synthesis and characterization of a hydroxyapatite/tenorite nanocomposite functionalized with folic acid, representing a nanotheranostic material with potential for application as an agent in positron emission tomography imaging systems and to act specifically in the treatment and diagnosis of osteosarcoma. 64Cu and 32P were produced by nuclear activation in the TRIGA reactor at CDTN. The obtained samples were characterized by XRD with Rietveld refinement, XAFS, SEM, BET, TGA, FTIR, CHN, ICP-AES, XPS and gamma spectroscopy. We investigated how CuO grows in HA NPs, the stability of the interactions between CuO and HA constituents and the interactions between folic acid and the surface of the HA NPs. The results indicate the formation of a second phase (tenorite) besides hydroxyapatite, and that the interactions between the two phases are stable, resulting in a nanocomposite. Furthermore, the activation of 64Cu and 32P inside the HA matrix, through the exposition to a neutron flux, produces a theranostic material of interest for biological tests. 相似文献
The development of a myriad of nanoparticles types has opened new possibilities for the diagnostics and treatment of many diseases, especially for cancer. However, most of the researches done so far do not focus on the protection of normal cells surrounding a tumor from irradiation bystander effects that might lead to cancer recurrence. Gap-junctions are known to be involved in this process, which leads to genomic instability of neighboring normal cells, and flufenamic acid (FFA) is included in a new group of gap-junction blockers recently discovered. The present work explores the use of mesoporous silica nanoparticles MCM-41 functionalized with 3-Aminopropyltriethoxysilane (APTES) for anchoring the flufenamic acid for its prolonged and controlled release and protection from radiation bystander effects. MCM-41 and functionalized samples were structurally and chemically characterized with multiple techniques. The biocompatibility of all samples was tested in a live/dead assay performed in cultured MRC-5 and HeLa cells. HeLa cells cultured were exposed to 50?Gy of gamma-rays and the media transferred to fibroblast cells cultured separately. Our results show that MCM-41 and functionalized samples have high biocompatibility with MCR-5 and HeLa cells, and most importantly, the FFA delivered by these NPs was able to halt apoptosis, one of main bystander effects. 相似文献
Reliability growth tests are often used for achieving a target reliability for complex systems via multiple test‐fix stages with limited testing resources. Such tests can be sped up via accelerated life testing (ALT) where test units are exposed to harsher‐than‐normal conditions. In this paper, a Bayesian framework is proposed to analyze ALT data in reliability growth. In particular, a complex system with components that have multiple competing failure modes is considered, and the time to failure of each failure mode is assumed to follow a Weibull distribution. We also assume that the accelerated condition has a fixed time scaling effect on each of the failure modes. In addition, a corrective action with fixed ineffectiveness can be performed at the end of each stage to reduce the occurrence of each failure mode. Under the Bayesian framework, a general model is developed to handle uncertainty on all model parameters, and several special cases with some parameters being known are also studied. A simulation study is conducted to assess the performance of the proposed models in estimating the final reliability of the system and to study the effects of unbiased and biased prior knowledge on the system‐level reliability estimates. 相似文献
Using the stacked crystal filter (SCF) concept, a coupled resonator filter (CRF) can be interpreted as a device in which 2 piezoresonators are stacked on top of each other in such a way that a certain degree of acoustic interaction occurs. The work presented in this paper reports a novel all-electrical model for the CRF. The model associates acoustical coupling with an equivalent electrical coupling between resonators. The resulting equivalent circuit makes it possible to apply classical filter synthesis techniques directly based on the coupling control between resonators. It complements with a synthesis approach the analysis approach of the Mason model. 相似文献
Urinary modified nucleosides have a potential role as cancer biomarkers, and most of the methods used in their study have utilized low-pressure phenylboronate affinity chromatography materials for the purification of the cis-diol-containing nucleosides. In this study, a boronate HPLC column was surprisingly shown not to trap the nucleosides as would be expected from experience with the classic Affigel 601 resin but showed only partial selectivity toward cis-diol groups while other groups exhibited better retention. In aprotic conditions, trapping of nucleosides was possible; however, the selectivity toward cis-diol-containing compounds was lost with the Lewis basicity of available nitrogens being the main determinant of retention. The experimental findings are compared to and confirmed by DFT calculations. 相似文献