Stable and transient subharmonic emissions from isolated contrast agent microbubbles

Ultrasound contrast agents (UCAs) have been widely studied in recent years in order to improve and develop new, sophisticated imaging techniques for clinical applications. In order to improve the understanding of microbubble-ultrasound interactions, an acoustic dynamic characterization of UCA microbubble behavior was performed in this work using a high frame-rate acquiring and processing system. This equipment is connected to a commercial scanner that provides RF beam-formed data with a frame-rate of 30 Hz. Acquired RF sequences allows us to follow the dynamics of cavitation mechanisms in its temporal evolution during different insonifying conditions. The experimental setup allowed us to keep the bubbles free in a spatial region of the supporting medium, thus avoiding boundary effects that can alter the ultrasound field and the scattered echo from bubbles. The work focuses on the study of subharmonic emission from an isolated bubble of contrast agent. In particular, the acoustic pressure threshold for a subharmonic stable emission was evaluated for a subset of 50 microbubbles at 3.3 MHz and at 5 MHz of insonation frequencies. An unexpected second pressure threshold, which caused the stand still of the subharmonic emission, was detected at 3.3 MHz and 5 MHz excitation frequencies. A transient subharmonic emission, which is hypothesized as being related to the formation of new free gas bubbles, was detected during the ultrasound-induced destruction of microbubbles. An experimental procedure was devised in order to investigate these behaviors and several sequences of RF echo signals and the related spectra, acquired from an isolated bubble in different insonation conditions, are presented and discussed in this paper.     

Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods—biomimetic (BioM), electrochemical at 20 mA/cm² and at 6.5 mA/cm²—and cultured with MSCs. At days 4, 7 and 14, cell proliferation—alamarBlue^® activity and DNA quantification—and differentiation down the osteogenic lineage—ALP activity normalised per amount of DNA and SEM (morphology)—were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.     

An empirical study on green practices of mobile phone users

Given the market’s saturation with smartphones and the increased power needs that these have as compared with older feature phones, the users’ green practices and behavior is emerging as an important research topic. The environmental aspects and general awareness issues are not addressed in this study; however, the limited battery life of smartphones is a decisive factor that shapes the users’ behavior, practices and preferences. As such, the users need to follow green practices and carefully assess the energy related characteristics (speed, screen size, weight, and price) that they value the most in a smartphone, to maximize their experience. Based on our previous work that gathered relevant data from 313 users, we extended the analysis in order to examine the user battery life and charging practices, buying habits, green practices and preferences. To that end, we used quantitative statistics and Fuzzy Decision Tree analysis to propose relevant Fuzzy Decision Rules that can classify the results and profile the users.     

Development of Concentration-Dependent Diffusion Instability in Reactive Miscible Fluids Under Influence of Constant or Variable Inertia

In this work, we focus on the processes which accompany a frontal neutralization reaction occurring between two miscible fluids filling a vertical Hele-Shaw cell. We have found that chemically-induced changes of reagent concentrations coupled with concentration- dependent diffusion (CDD) can produce spatially localized low density areas which are sensitive to the external inertial field. In the case of static gravity we have demonstrated both experimentally and theoretically that it can give rise to the development of perfectly periodic convective structure. This scenario is strikingly different from the irregular density fingering, which is typically observed in the miscible systems. When the system is under the influence of the periodic low-frequency vibrations perpendicular to the reaction front, we found numerically the excitation of a mixed-mode instability combining the double-diffusion instabilities and the Rayleigh-Taylor mechanism of the convection within the low density areas.     

Ecoepidemics with infected prey in herd defence: the harmless and toxic cases

We consider a predator–prey population model with prey gathering together for defence purposes. A transmissible unrecoverable disease affects the prey. We characterize the system behaviour, establishing that ultimately either only the susceptible prey survive, or the disease becomes endemic, but the predators are wiped out. Another alternative is that the disease is eradicated, with sound prey and predators thriving at an equilibrium or through persistent population oscillations. Finally, the populations can thrive together, with the endemic disease. The only impossible alternative in these circumstances is predators thriving just with infected prey. But this follows from the model assumptions, in that infected prey are too weak to sustain themselves. A mathematical peculiarity of the model is the singularity-free reformulation, which leads to three entirely new dependent variables to describe the system. The model is then extended to encompass the situation in which ingestion of diseased prey is fatal for the predators and to the cases where the predators find the infected prey less palatable.     

A software engineering approach for the development of heterogeneous robotic applications

One of the most evident characteristics of robotic applications is heterogeneity: large robotic projects involve many different researchers with very different programming needs and areas of research, using a variety of hardware and software that must be integrated efficiently (i.e.: with a low development cost) to construct applications that satisfy not only classic robotic requirements (fault-tolerance, real-time specifications, intensive access to hardware, etc.) but also software engineering aspects (reusability, maintainability, etc.). Most existing solutions to this problem either do not deal with such heterogeneity or do not cover specific robotic needs. In this paper we propose a framework for the integration of heterogeneous robotic software through a software engineering approach: the BABEL development system, which is aimed to cover the main phases of the application lifecycle (design, implementation, testing, and maintainance) when unavoidable heterogeneity conditions are present. The capabilities of our system are shown by its support for designing and implementing diverse real robotic applications that use several programming languages (C, C++, JAVA), execution platforms (RT-operating systems, MS-Windows, no operating system at all), communication middleware (CORBA, TCP/IP, USB), and also a variety of hardware components (Personal Computers, microcontrollers, and a wide diversity of sensor and actuator devices in mobile robots and manipulator arms).