Reduced Gonadotrophin Receptor Expression Is Associated with a More Aggressive Ovarian Cancer Phenotype

Follicle-stimulating hormone (FSH) and luteinising hormone (LH) play important roles in regulating cell growth and proliferation in the ovary. However, few studies have explored the expression of FSH and LH receptors (FSHR and LHCGR) in ovarian cancer, and their functional roles in cancer progression remain inconclusive. This study investigated the potential impact of both mRNA (FSHR, LHCGR) and protein (FSHR, LHCGR) expression on ovarian cancer progression using publicly available online databases, qRT-PCR (high grade serous ovarian cancers, HGSOC, n = 29 and benign ovarian tumors, n = 17) and immunohistochemistry (HGSOC, n = 144). In addition, we investigated the effect of FSHR and LHCGR siRNA knockdown on the pro-metastatic behavior of serous ovarian cancer cells in vitro. High FSHR or high LHCGR expression in patients with all subtypes of high-grade ovarian cancer was significantly associated with longer progression-free survival (PFS) and overall survival (OS). High FSHR protein expression was associated with increased PFS (p = 0.050) and OS (p = 0.025). HGSOC patients with both high FSHR and high LHCGR protein levels had the best survival outcome, whilst both low FSHR and low LHCGR expression was associated with poorest survival (p = 0.019). Knockdown of FSHR significantly increased the invasion of serous ovarian cancer cells (OVCAR3 and COV362) in vitro. LHCGR knockdown also promoted invasion of COV362 cells. This study highlights that lower FSHR and LHCGR expression is associated with a more aggressive epithelial ovarian cancer phenotype and promotes pro-metastatic behaviour.     

Reducing the burden of HACCP on SMEs

Despite the acknowledged contribution of Small and Medium Enterprises (SMEs) to the food industry there is increasing evidence that Hazard Analysis Critical Control Point (HACCP) implementation is limited in this sector, with the burden of implementation perceived as potentially insurmountable. Using an action research methodology, this in-depth, government funded research project took the form of a two stage, 18 month investigation into methods of reducing burdens of HACCP on SMEs. Stage one indicted that SMEs see HACCP as a difficult, complex set of activities requiring great amounts of time effort and with few, if any, perceived benefits. In stage two, however, with the help of research tools developed, a number of SMEs completed HACCP and many made good progress on a tight timescale. This research thus concludes that SMEs can achieve HACCP if they are provided with sufficient guidance and support in a context of general consensus of HACCP terminology and requirements. Recommendations are made, many of which have been subsequently adopted by the UK Food Standards Agency.     

基于SuperMap的地理信息系统课程教学设计

根据地理信息系统课程的特点,提出基于SuperMap的课程教学设计,将相关的GIS理论、算法、过程和结果输出与显示的一系列基础性实验、综合性实验、设计性实验得以直观的实现．针对课程教学中出现的问题,对课程的设计理念、教学目标、教学内容、教学模式等进行分析研究,在GIS教学过程中,在对内容的安排上紧跟社会需求和科学技术的发展,实现缩短学时,提高教学效果,优化教学过程,增强学生信息素养,提高学生综合竞争能力．     

Modelling and management of smart microgrid for rural electrification in sub-saharan Africa: The case of Nigeria

Access to electricity is still a challenge in many parts of sub-Saharan Africa. In Nigeria, over 70% of the rural dwellers do not have access to electricity. The purpose of this paper is to examine the potential of a smart microgrid for off-grid rural electrification in Nigeria. A combination of design thinking and model-based design methodology is employed to select a suitable microgrid configuration and to develop a smart microgrid model. A system consisting of a solar photovoltaic array, battery energy storage and a diesel generator is selected, and the model is developed in Simulink. Demand data from 10 rural communities in Nigeria are used to validate the performance of the model and the potential for demand management is considered. The use of energy efficient light bulbs is found to reduce the peak electricity demand of the case study communities by 42 to 76%. Combining the proposed system with the use of LED bulbs makes the system to have 56 to 81% less net present cost than a system with a diesel generator alone and incandescent light bulbs. The proposed smart microgrid is found to be more suitable for off-grid rural electrification in Nigeria than diesel generators which are currently used for off-grid electrification in Nigeria.     

Marching Cubes算法改进研究及应用

对MC算法进行了改进和优化,运用渐近线判别法解决了原有算法的二义性问题,改善了生成图形的质量;利用线性八叉树结构提高了图形的绘制速度,使实时绘制和交互操作成为可能。运用改进后的MC算法,结合某铁矿实测数据进行矿体表面三维重构,得到了满意的效果。     

Temporal Bayesian Knowledge Bases – Reasoning about uncertainty with temporal constraints

Time is ubiquitous. Accounting for time and its interaction with change is crucial to modeling the dynamic world, especially in domains whose study of data is sensitive to time such as in medical diagnosis, financial investment, and natural language processing, to name a few. We present a framework that incorporates both uncertainty and time in its reasoning scheme. It is based on an existing knowledge representation called Bayesian Knowledge Bases. It provides a graphical representation of knowledge, time and uncertainty, and enables probabilistic and temporal inferencing. The reasoning scheme is probabilistically sound and the fusion of temporal fragments is well defined. We will discuss some properties of this framework and introduce algorithms to ensure groundedness during the construction of the model. The framework has been applied to both artificial and real world scenarios.     

Overlapping melodic alarms are almost indiscriminable

OBJECTIVE: We explore how accurately and quickly nurses can identify melodic medical equipment alarms when no mnemonics are used, when alarms may overlap, and when concurrent tasks are performed. BACKGROUND: The international standard IEC 60601-1-8 (International Electrotechnical Commission, 2005) has proposed simple melodies to distinguish seven alarm sources. Previous studies with nonmedical participants reveal poor learning of melodic alarms and persistent confusions between some of them. The effects of domain expertise, concurrent tasks, and alarm overlaps are unknown. METHOD: Fourteen intensive care and general medical unit nurses learned the melodic alarms without mnemonics in two sessions on separate days. In the second half of Day 2 the nurses identified single alarms or pairs of alarms played in sequential, partially overlapping, or nearly completely overlapping configurations. For half the experimental blocks nurses performed a concurrent mental arithmetic task. RESULTS: Nurses' learning was poor and was no better than the learning of nonnurses in a previous study. Nurses showed the previously noted confusions between alarms. Overlapping alarms were exceptionally difficult to identify. The concurrent task affected response time but not accuracy. CONCLUSION: Because of a failure of auditory stream segregation, the melodic alarms cannot be discriminated when they overlap. Directives to sequence the sounding of alarms in medical electrical equipment must be strictly adhered to, or the alarms must redesigned to support better auditory streaming. APPLICATION: Actual or potential uses of this research include the implementation of IEC 60601-1-8 alarms in medical electrical equipment.     

Cadmium-binding protein components of flaxseed: Influence of cultivar and location

The distribution of cadmium-(Cd)-binding components from five flaxseed cultivars grown at three locations in southern Manitoba was investigated to examine genotypic and environmental effects. Three protein fractions with different electrostatic properties, eluting at 0.10, 0.25 and 0.50 M NaCl by ion-exchange chromatography DEAE-Sephacel, represented 12%, 66% and 7% of the bound (extracted) protein, respectively, while 15% of the protein remained unbound. Cadmium and other divalent metal (zinc, copper and calcium) contents of protein fractions were strongly influenced by location. Cultivar differences in protein and cadmium contents of the protein fractions were highly significant. Cadmium and zinc accumulated similarly in the 0.10 and 0.25 M protein fractions at 51% and 40–43%, respectively. Transfer of copper occurred prominently in the 0.50 M fraction while most of the calcium (55%) remained unbound. The distribution of cadmium, zinc, copper and calcium in fractions of flaxseed proteins was strongly influenced by cultivar and location, indicating differences in their accumulation, migration and transfer.     

Effect of ripeness and postharvest storage on the evolution of colour and anthocyanins in cherries (Prunus avium L.)

The relationship between colour parameters and anthocyanins of four sweet cherry cultivars, Burlat, Saco, Summit and Van was studied. The colour (L^∗, a^∗, b^∗, chroma and hue angle parameters) and anthocyanins were analysed during two different years at two different ripening stages (partially ripe, and ripe, respectively). The cherries were analysed at harvest and after storage at 1.5 ± 0.5 °C and 15 ± 5 °C for 30 and 6 days, respectively. The colour was measured by tristimulus colourimetry (CIELAB system) directly on the fruits, while anthocyanins were quantified by HPLC-DAD analysis on methanolic extracts of freeze-dried samples of the fresh cherries and on the differently stored cherries. L^∗, chroma, and hue angle values were always lower for the ripe than for the partially ripe cherries. All of the cultivars were found to contain cyanidin-3-rutinoside and cyanidin-3-glucoside as the major anthocyanins. The total anthocyanin content in fruits of the different cultivars varied in the order Burlat > Saco > Van > Summit. The concentration of anthocyanins increased at both temperatures of storage in both ripe and partially ripe cherries, but the extent of increase varied among cultivars. Cherries stored at 15 ± 5 °C showed higher reduction of L^∗, chroma and hue angle than fruits stored at 1.5 ± 0.5 °C. L^∗, a^∗, b^∗, chroma and hue angle correlated negatively (P < 0.001) with the total anthocyanins levels, but not with the total phenols. These results show that chromatic functions of chroma and hue correlate closely with the evolution of colour and anthocyanins levels during storage of sweet cherries and indicate that colour measurements can be used to monitor pigment evolution and anthocyanin contents of cherries (and vice versa).     

Postharvest Quality of Strawberry Fruit (<Emphasis Type="Italic">Fragaria x Ananassa</Emphasis> Duch cv. Albion) as Affected by Ozone Washing: Fungal Spoilage,Mechanical Properties,and Structure

Storage life of strawberry fruit is limited due to its high softening rate and susceptibility to fungal attack. Thus, it is of interest to develop and improve preservation techniques to reduce postharvest losses. The aim of this study was to evaluate the effect of ozonized water washing (maximum concentration of 3.5 mg L^?1; 5–15 min) on fungal spoilage, water loss, mechanical properties, and structure of strawberries (cv. Albion) harvested in different years and stored at 5 °C. The effect of ozone treatment on decontamination and weight loss was dose-dependent. Ozonization for 5 min significantly reduced fungal incidence (~?22–25% lesser than control) and weight loss of strawberries throughout storage. High doses (10 or 15 min) did not reduce significantly fungal growth in relation to control, although weight loss in fruit treated for 15 min was higher than control. In both harvest years studied, cold storage provoked a diminution in deformability modulus and an increase in deformation but a significant effect of treatment was not observed. These changes were correlated with alteration of cell walls and turgor loss. Otherwise, cuticular membrane of strawberries treated for 5 min was observed thicker and more electron dense than in fruit ozonized for 15 min or control. It could partially explain the lower moisture loss observed in 5-min treated fruit. Results showed that ozonized water washing for 5 min can be an alternative strategy for extending postharvest strawberry shelf-life by decreasing fungal decay and water loss along cold storage, without affecting mechanical parameters.