Validation of a 1-Day Analytical Diagnostic Real-Time PCR for the Detection of Salmonella in Different Food Meat Categories

Foodborne disease caused by Salmonella represents a worldwide public health problem. In Europe, salmonellosis is still the second most commonly recorded zoonosis. Since the standard culture method for detecting Salmonella (ISO 6579:2002) requires up to 5 days to produce results, the need to develop rapid methods represents an important issue for the authorities and the producers. The aim of the present study was the in-house validation, according to ISO 16140, of an open-formula diagnostic real-time PCR for the detection of Salmonella in all the different meat categories reported in the EU Regulations relative to microbiological criteria for food safety. The assay employed specific primers and a probe target within the ttrRSBCA locus, which allows the tetrathionate respiration in Salmonella. Selectivity, relative accuracy, relative sensitivity and relative specificity were established by testing 110 bacterial strains and 175 various edible meat samples. Results showed 100 % selectivity, 100 % relative accuracy, 100 % relative sensitivity and 100 % relative specificity of the real-time PCR when compared to the standard culture method used as reference. In addition, in order to minimize the effect of the competitive micro-flora naturally present on meat samples, a highly nutritious and selective commercial medium (ONE Broth Salmonella, Oxoid) was evaluated in comparison with the classical non-selective pre-enrichment broth (buffered peptone water). Results demonstrated that the ONE Broth Salmonella medium increases the growth of Salmonella in the presence of competitive micro-flora.     

Anomalous weak values via a single photon detection

Is it possible that a measurement of a spin component of a spin-1/2 particle yields the value 100? In 1988 Aharonov, Albert and Vaidman argued that upon pre-and...     

In search of a reliable technique for the determination of the biological stability of the organic matter in the mechanical-biological treated waste

The biological stability determines the extent to which readily biodegradable organic matter has decomposed. In this work, a massive estimation of indices suitable for the measurement of biological stability of the organic matter content in solid waste samples has been carried out. Samples from different stages in a mechanical-biological treatment (MBT) plant treating municipal solid wastes (MSW) were selected as examples of different stages of organic matter stability in waste biological treatment. Aerobic indices based on respiration techniques properly reflected the process of organic matter biodegradation. Static and dynamic respirometry showed similar values in terms of aerobic biological activity (expressed as oxygen uptake rate, OUR), whereas cumulative oxygen consumption was a reliable method to express the biological stability of organic matter in solid samples. Methods based on OUR and cumulative oxygen consumption were positively correlated. Anaerobic methods based on biogas production (BP) tests also reflected well the degree of biological stability, although significant differences were found in solid and liquid BP assays. A significant correlation was found between cumulative oxygen consumption and ultimate biogas production. The results obtained in this study can be a basis for the quantitative measurement of the efficiency in the stabilization of organic matter in waste treatment plants, including MBT plants, anaerobic digestion of MSW and composting plants.     

DAWL: A Delayed-ACK Scheme for MAC-Level Performance Enhancement of Wireless LANs

The IEEE 802.11 MAC protocol provides a reliable link layer using Stop & Wait ARQ. The cost for high reliability is the overhead due to acknowledgement packets in the direction opposite to the actual data flow. In this paper, the design of a new protocol as an enhancement of IEEE 802.11 is proposed, with the aim of reducing supplementary traffic overhead and increasing the bandwidth available for actual data transmission. The performance of the proposed protocol is evaluated through comparison with IEEE 802.11 as well as with a SSCOP-based protocol. Results underline significant advantages of the proposed protocol against existing ones, thus confirming the value and potentiality of the approach.Dzmitry Kliazovich received his Masters degree in telecommunication science from Belarusian State University of Informatics and Radioelectronics in 2002. He is currently working towards the Ph.D. degree in University of Trento, Italy. His main research interest lies in wireless networking field with a focus on performance optimization and cross-layer design.Fabrizio Granelli was born in Genoa in 1972. He received the “Laurea” (M.Sc.) degree in Electronic Engineering from the University of Genoa, Italy, in 1997, with a thesis on video coding, awarded with the TELECOM Italy prize, and the Ph.D. degree in Electronic Engineering and Computer Science from the same university in 2001. Since 2000 he is carrying on his teaching activity as Assistant Professor at the Dept. of Information and Communication Technologies (DIT) of the University of Trento (Italy) within the B.Sc. and M.Sc. Degrees in Telecommunications Engineering.The research interests of Dr. Granelli are mainly focused on networking, with particular attention to network modeling and performance evaluation, wireless networks, access control, and next-generation telecommunication networks.He is author of more than 30 refereed papers, published in several international journals and conferences.Dr. Granelli is member of the IEEE Committee on “Communication Systems Integration and Modeling” (CSIM) and of the Technical Programme Committee of the “QoS and Performance Evaluation Symposium” of the International Conference on Communications (ICC 2003 and ICC 2004).     

Integrating load balancing and locality in the parallelization of irregular problems

An irregular problem models the evolution of a system where several elements are irregularly distributed in a domain. The evolution modifies this distribution in a way that cannot be foreseen and the behavior of each element depends upon the elements close to it according to a problem dependent relation. Starting from a hierarchical representation of the domain, we define a parallelization methodology that includes a load balancing strategy that preserves this locality property and a strategy to collect information distributed onto the processing nodes.     

Planar packing of trees and spider trees

In [A. García, C. Hernando, F. Hurtado, M. Noy, J. Tejel, Packing trees into planar graphs, J. Graph Theory (2002) 172-181] García et al. conjectured that for every two non-star trees there exists a planar graph containing them as edge-disjoint subgraphs. In this paper we prove the conjecture in the case in which one of the trees is a spider tree.     

SVD-Based Techniques for Zero-Padded Block Transmission Over Fading Channels

In this paper, the performance of filter bank transceivers in the presence of a dispersive time-variant channel is investigated. It is well known that filter bank transceivers can be adapted to the channel transfer function to yield intersymbol interference (ISI) cancellation. When the channel is time variant, several problems arise, since the transceiver should be changed whenever the channel evolves. In this paper, we will allow both the transmitter and the receiver to change and satisfy the interference-free condition, under the assumption of a zero-padded block transmission. In this case, the optimum transmitter-receiver pair can be computed by using a singular value decomposition (SVD) of the channel matrix. A fast receiver adaptation based on SVD tracking is presented. Simulation results show that minimum performance loss with respect to the optimum receiver can be achieved for our reduced complexity receiver