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201.
B Kirel S Yetgin U Saatci S Ozen A Bakkaloglu N Besbas 《Canadian Metallurgical Quarterly》1996,15(3):236-241
Brush border myosin-I (BBMI) is a single-headed unconventional myosin found in the microvilli of intestinal epithelial cells, where it links the core bundle of actin filaments to the plasma membrane. An association of BBMI with anionic phospholipids has been shown to be mediated by a carboxy-terminal domain which is rich in basic amino acids. We have exploited this natural affinity of BBMI for negatively charged lipids to form two-dimensional (2D) crystals of this protein which are suitable for structural analysis by electron crystallographic techniques. The 2D crystals which we have obtained belong to one of two space groups, p22121 or p2. We present here projection maps calculated from images of negatively stained crystals for each of these crystal types to a resolution of 20 A and show that the asymmetric unit is the same in both crystal types. 相似文献
202.
U. K. Viswanathan T. R. G. Kutty R. Keswani C. Ganguly 《Journal of Materials Science》1996,31(10):2705-2709
The hardening response and the indentation creep of a 350 grade commercial maraging steel were evaluated using a hot hardness tester. The hardness versus temperature plot exhibited three distinct regions. Hardness response was noted between 500–800 K. The unusually high values of activation energy and stress exponent obtained during the creep experiment could be rationalized by a novel concept of introducing a back stress term in the indentation creep relation. The corrected value of the activation energy was found to be reasonably in agreement with the activation energy for diffusion of Ni in iron. Results are supplemented with microstructural observation. 相似文献
203.
RAP46 is a negative regulator of glucocorticoid receptor action and hormone-induced apoptosis 总被引:1,自引:0,他引:1
M Kullmann J Schneikert J Moll S Heck M Zeiner U Gehring AC Cato 《Canadian Metallurgical Quarterly》1998,273(23):14620-14625
RAP46 was first identified by its ability to bind the glucocorticoid receptor. It has since been reported to bind several cellular proteins, including the anti-apoptotic protein Bcl-2, but the biological significance of these interactions is unknown. Here we show that RAP46 binds the hinge region of the glucocorticoid receptor and inhibits DNA binding and transactivation by the receptor. We further show that overexpression of RAP46 in mouse thymoma S49.1 cells inhibits glucocorticoid-induced apoptosis. Conversely, glucocorticoid-induced apoptosis and transactivation were enhanced after treating S49.1 cells with the immunosuppressant rapamycin, which down-regulates cellular levels of BAG-1, the mouse homolog of RAP46. The effect of rapamycin can, however, be overcome by overexpression of RAP46. These results together identify RAP46 as a protein that controls glucocorticoid-induced apoptosis through its negative regulatory action on the transactivation property of the glucocorticoid receptor. 相似文献
204.
M Karlberg M Magnusson EM Malmstr?m A Melander U Moritz 《Canadian Metallurgical Quarterly》1996,77(9):874-882
OBJECTIVE: To assess postural performance in patients with dizziness of suspected cervical origin in whom extracervical causes had been excluded, and to assess the effects of physiotherapy on postural performance and subjective complaints of neck pain and dizziness. DESIGN: Prospective, randomized, controlled trial. SETTING: Primary care centers and a tertiary referral center. PATIENTS AND SUBJECTS: Of 65 referrals, 43 patients were excluded because extracervical etiology was suspected. Of the remaining 22 patients, 17 completed the study (15 women, 2 men, x age 37 yr, range 26-49). The controls were 17 healthy subjects (15 women, 2 men, x age 36 yr, range 25-55). INTERVENTION: Physiotherapy based on analysis of symptoms and findings, and aimed to reduce cervical discomfort. Patients were randomized either to receive immediate physiotherapy (n = 9), or to wait 2 months, undergo repeat measurements, and then receive physiotherapy (n = 8). MAIN OUTCOME MEASURES: Posturography, measuring velocity and variance of vibration-induced body sway and variance of galvanically induced body sway. Subjective intensity of neck pain (Visual Analog Scale ratings, 0-100), intensity and frequency of dizziness (subjective score 0-4). RESULTS: The patients manifested significantly poorer postural performance than did healthy subjects (.05 > p > .0001). Physiotherapy significantly reduced neck pain and intensity and the frequency of dizziness (p < .01), and significantly improved postural performance (.05 > p > .0007). CONCLUSIONS: Patients with dizziness of suspected cervical origin are characterized by impaired postural performance. Physiotherapy reduces neck pain and dizziness and improves postural performance. Neck disorders should be considered when assessing patients complaining of dizziness, but alternative diagnoses are common. 相似文献
205.
The present study was initiated to assess time-course and risk factors for the development of cataract and posterior-capsule opacification as well as complications of cataract surgery and YAG-laser capsulotomy following vitrectomy for diabetic retinopathy. The charts of all patients undergoing vitrectomy for diabetic retinopathy during a 5-year period in a university eye hospital were retrospectively reviewed. The course of 306 consecutive eyes in which the lens was retained during vitrectomy was analyzed for subsequent cataract surgery and YAG-laser capsulotomy. The first 6 months after cataract or YAG-laser surgery were examined for the occurrence of complications. Data were analyzed with regard to the time course using Kaplan-Meier life-table analysis. The proportion of eyes that underwent cataract surgery after vitrectomy increased nearly linearly with time, approaching 75% after 5 years. Silicone tamponade (relative risk 1.9; P = 0.0005) and transscleral retinal cryotherapy (relative risk 1.4; P = 0.003) were risk factors for subsequent cataract surgery. No significant cataractogenous effect of intravitreal gas as compared with balanced salt solution was found. YAG-laser capsulotomy was performed in 60% of vitrectomized diabetic eyes within 2 years but in only 10% of nondiabetic controls (P < 0.0001). Within 6 months of extracapsular cataract surgery with implantation of an intraocular lens (IOL) in 54 eyes, no serious complication was observed. After YAG-laser capsulotomy, vitreous hemorrhage occurred within 6 months in 6 of 21 eyes. In conclusion, cataract surgery was performed in 75% of the phakic eyes within 5 years of vitrectomy for diabetic retinopathy. Posterior capsular opacification is particularly common in this subset of eyes. No serious complication was observed after extracapsular cataract surgery with IOL implantation, but YAG-laser capsulotomy was associated with an increased risk for vitreous hemorrhage. 相似文献
206.
U. Lembke K. Grosse R. Pascova I. Gutzow O. Becker L. Horn 《Journal of Materials Science》1996,31(16):4333-4346
The kinetics of silver chloride cluster formation in a sodium borate glass heat treated isothermally at different temperatures above the glass transition temperature has been studied with small-angle X-ray scattering (SAXS). It is established that the size distributions of particle number and particle volume have bimodal shapes, i.e. the system of AgCl droplets consists of two populations with significantly different mean radii. The bimodality of the size distribution functions is confirmed by results of transmission electron microscopy (TEM) even if different techniques of sample preparation are used. The resolution limits of the TEM techniques applied amount to 2 nm and are comparable with the smallest particle diameters detectable by the SAXS method. The evolution of the size distributions is discussed in the framework of the theories of nucleation, growth and Ostwald ripening. 相似文献
207.
Large eddy simulation of the free-shear flows has been performed using an algebraic subgrid stress model. The test cases considered are the temporal planar jet and mixing layer. The results are compared with that of the Smagorinsky, dynamic Smagorinsky, mixed models and the DNS data. For the mean flow quantities none of the models perform exceptionally better over the others. However, both the algebraic and mixed models predict better statistics for the turbulent quantities than the linear models. The results also demonstrate the capability of the algebraic model in depicting backscatter of energy explicitly, which is consistent in both the cases considered. 相似文献
208.
209.
Parallelization of the global extremum searching process 总被引:1,自引:0,他引:1
Yu. G. Evtushenko V. U. Malkova A. A. Stanevichyus 《Automation and Remote Control》2007,68(5):787-798
The parallel algorithm for searching the global extremum of the function of several variables is designed. The algorithm is based on the method of nonuniform coverings proposed by Yu.G. Evtushenko for functions that comply with the Lipschitz condition. The algorithm is realized in the language C and message passing interface (MPI) system. To speed up computations, auxiliary procedures for founding the local extremum are used. The operation of the algorithm is illustrated by the example of atomic cluster structure calculations. 相似文献
210.
Application of stochastic filtering for lifetime prediction 总被引:1,自引:0,他引:1
This paper introduces a stochastic filtering modeling approach for predicting the remaining lifetime of a component based on information on the stochastic degradation process and uncertain condition monitoring measurements. The model is illustrated by a case study, where the degradation is assumed to be a simplified fatigue crack growth process. The model accounts for uncertainties in both degradation process and condition measurements in a sound way. If completed with information on costs of monitoring, failure and replacement, such model could be used in optimizing both the condition monitoring intervals and, e.g. the replacement time for the component. 相似文献