Mesothelial morphology and organisation after peritoneal treatment with solid and liquid adhesion barriers–a scanning electron microscopical study

Separation of traumatized tissue represents the only promising strategy in postoperative adhesion prevention, a relevant clinical problem after surgical intervention. In the present study scanning electron microscopy (SEM) and subsequent morphometry were used to analyse the tissue response to five commercial adhesion barriers. Standardised peritoneal lesions in Wistar rats were covered with solid and viscous barrier materials and semiquantitatively analysed 14 days postoperatively. Striking morphological differences in lesion surface organisation between the barrier groups became apparent with colonisation of the barrier by mesothelial cells to different degrees. Furthermore, the mesothelial cells showed either a normal or activated phenotype depending on the underlying biomaterial. These experiments demonstrate that the examination by SEM gives useful insights into the performance of barrier materials and the cellular processes of adhesion prevention, since mesothelial cells play an active role in the pathogenesis of adhesion formation.     

Lamb waves for non-contact fatigue state evaluation of composites under various mechanical loading conditions

Methodologies for non-destructive evaluation of mechanically induced fatigue in fibre reinforced polymers are discussed. Specimens made of non-crimp glass fabric are fatigued using three different load ratios (tension–tension, tension–compression, and compression–compression). The investigation involves two loading directions (0° and 90°) of the quasi–orthotropic composite. Based on mode conversion of air-coupled ultrasound to Lamb waves, variation in a₀-mode velocity is measured in a non-contact and single-sided access configuration. The velocity measurements are performed within and outside the servo-hydraulic test rig used for inducing fatigue damage. Formation of cracks monitored in the transparent composite results in degradation of stiffness observed by the test rig. Decrease in a₀-mode velocity caused by fatigue is shown to correlate closely with stiffness degradation for all loading ratios and directions. The correlation is studied by calculating a₀-mode velocities from single-ply properties whose stiffness degradation was determined using the observed crack densities and a finite element based model.     

Effects of axial, transverse, and oblique sample motion in FD OCT in systems with global or rolling shutter line detector

This study deals with effects on the interference signal caused by axial, transverse, and oblique motion in spectrometer-based Fourier-domain optical coherence tomography (FD OCT). Two different systems are compared-one with a global shutter line detector and the other with a rolling shutter. We present theoretical and experimental investigations of motion artifacts. Regarding axial motion, fringe washout is observed in both systems, and an additional Doppler frequency shift is seen in the system using a rolling shutter. In addition, both systems show the same SNR decrease as a result of a transversely and obliquely moving sample. The possibility of flow measurement by using the decrease in signal power was demonstrated by imaging 1% Intralipid emulsion flowing through a glass capillary. This research provides an understanding of the SNR degradation caused by sample motion and demonstrates the importance of fast data acquisition in medical imaging.     

Diversity detection of speckles for double-wavelength interferometry on rough surfaces

When the topography of a rough surface is measured with a double-wavelength interferometer, the phase error of the signal corresponding to the synthetic wavelength increases in the vicinity of dark speckles. To overcome this problem we perform an amplitude-dependent averaging of the synthetic phase over independent speckles (diversity detection). We either use spatially neighboring speckles or in the case of depolarizing surfaces, we use speckles of the same spatial mode, but with orthogonal polarizations. For the latter case the lateral resolution stays unaffected. The reduction of the speckle noise is demonstrated experimentally for a laterally scanning double-wavelength interferometer with superheterodyne detection of the synthetic phase.     

The alcohol-related accident risk in Germany: procedure, methods and results

This paper presents the first reliable estimation of the alcohol-related accident risk in Germany by comparing a representative sample of accidents to a representative sample of trips not leading to a crash. The information about the trips was taken from the German Roadside Survey 1992-1994 (n=9087) conducted in Unterfranken, part of Bavaria. These data were weighted according to a representative study of driving in Germany (KONTIV 89). The accident study comprises a representative sample of accidents in Unterfranken in 1993 (n=1968). Relating accident risk to BAC, the global risk function indicates an exponential increase of accident risk for BACs above 0.05%. Controlling for correlating factors leads to an overall lower estimation with, however, the same structure, indicating that alcohol is consumed by drivers in circumstances which further increase the risk introduced by alcohol. Analyzing the attributable risk (AR) shows that about 12% of all accidents are attributable to alcohol. Over 96% of these happen with BACs of 0.05% and above. Thus, measures aimed at reducing the alcohol-related accident risk must focus on larger BACs, especially of 0.08% and above.     

Effect of heat treatment on morphology and thermal decomposition kinetics of multiwalled carbon nanotubes

High temperature treatment in inert atmosphere proved to be an effective way to improve high temperature stability of MWNTs in ambient condition. TEM analysis of heat-treated MWNTs confirmed successful removal of impurities and formation of ordered graphene layers and internal bamboo structure. TG–DTG curves indicated that decomposition range and rate of as-received MWNTs were narrow and notably higher, respectively, than heat-treated MWNTs mainly due to presence of impurities like metal nanoparticles in the former. Non-isothermal kinetic analysis revealed that the rate determining mechanism for as-received MWNTs was random nucleation and growth of active species. However, for heat-treated MWNTs, rate controlling mechanism was chemical reaction. Higher activation energies (～203 kJ mol^?1 and 280 kJ mol^?1) and reaction orders (3 and 4) of MWNTs heat-treated at 1200 °C and 1800 °C in inert, respectively, indicated delayed thermal decomposition than as-received MWNTs (E_a ≈ 178 kJ mol^?1; n = 1) even in oxidative atmosphere.     

Diffractive simultaneous bidirectional shearing interferometry using tailored spatially coherent light

Measurements of wavefront deformations can be carried out with the help of lateral shearing interferometers. Here the focus is on a setup providing two shears along orthogonal directions simultaneously to generate the data needed for a reconstruction. We describe a diffractive solution using Ronchi phase gratings with a suppressed zeroth order for both the doubling of the wavefront under test and the bidirectional shearing unit. A series arrangement of the gratings offers an on-axis geometry, which minimizes the systematic errors of the test. For illumination, an extended incoherent monochromatic light source is used. High-contrast fringes can be obtained by tailoring the degree of coherence via a periodic intensity distribution.     

Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry

Little is known about the occurrence and the fate of veterinary drugs in the environment. Therefore, a liquid chromatography/tandem mass spectrometry method was developed and employed to investigate in detail the distribution and persistence of the frequently used tetracyclines and tylosin in a field fertilized with liquid manure on April 2000 and April 2001; soil sampling was performed in May 2000, November 2000, and May 2001. We detected 4.0 mg/kg tetracycline and 0.1 mg/kg chlortetracycline in the liquid manure of April 2000, as well as comparable amounts in the liquid manure of April 2001. In the soil samples of May 2001, the highest average concentrations of 86.2 (0-10 cm), 198.7 (10-20 cm), and 171.7 microg/kg (20-30 cm) tetracycline and 4.6-7.3 micro/kg chlortetracycline (all three sublayers) were found. At soil depths between 30 and 90 cm, as well as in soil or groundwater, tetracyclines could not be detected. In addition, oxytetracycline and tylosin could not be detected in any sample investigated. We conclude that tetracyclines enter the environment in significant concentrations via repeated fertilizations with liquid manure, build up persistent residues, and accumulate in soil. Therefore, tetracyclines may have a potential risk and investigations on the environmental effects of these antibiotics are necessary.     

Preparation of YBa2Cu3O7−x by thermal decomposition of coprecipitated oxalates

Yttrium, barium and copper oxalates are coprecipitated quantitatively from metal acetate solutions with oxalic acid in water-acetone mixtures. The particle-size distribution of the precipitate can be controlled by the precipitation conditions. Thermal decomposition of the coprecipitate leads to single-phase YBa₂Cu₃O_7-x . Despite the intermediate formation of BaCO₃, the minimal reaction temperature (770 °C) is clearly lower than that for the solid-state reaction. Results of differential thermal analysis and isothermal thermogravimetry are discussed. The products are characterized by X-ray diffraction and infrared spectroscopy.