近距离下覆4#煤层综采工作面顶板管理经验

介绍了近距离深层综采工作面顶板和巷道顶板管理经验，及综采工作面在通过巷道冒项区域时所采取的办法和措施。     

Widespread posttraumatic spinal subdural hematoma--imaging findings with spontaneous resolution: case report

A 63-year-old man developed paraparesis and signs of meningeal irritation 4 days after a fall which caused a minor contusion of the cervical spine. Magnetic resonance imaging (MRI) revealed an extensive spinal subdural hematoma. The usefulness of MRI for diagnosis and successful conservative treatment is discussed.     

提高矿井通风系统的安全和可靠性

分析了矿井通风系统存在的问题 ,针对性地提出了一些提高矿井通风系统安全及可靠性的措施     

浅谈如何做一名新时期创新型教师

教师的职业道德是贯穿教育全过程的精神支柱,它对学生和社会都会有深远的激励和影响作用;而熟练掌握专业知识和教育理论知识是教师业务能力的本质要求。新时期的创新型教师都必须不断地在这两个方面进行提升才能够符合时代的要求。     

组态软件在应用中的功能扩展

在DDE动态数据交换基础上,采用本地以太网UDP用户数据报协议,解决了组态王不支持网络变量从本机输出的问题,完善了系统功能。     

光纤光栅最佳切趾函数的研究

采用耦合模理论，给出了用传输矩阵法计算光纤Bragg光栅（FBG）特性的方法，进而得到了在不同切趾函数形式下的FBG反射谱的数值解。通过理论分析，给出了最佳切趾函数反射谱和群时延特性曲线。     

Extrathymic differentiation of resident T cells in the joints of mice with collagen-induced arthritis

Murine collagen-induced arthritis (CIA) is known as a T cell-mediated autoimmune disease, although autoantibodies are also suspected to be associated with the onset of the disease. To determine the origin of such T cells in the joints of mice with CIA, their phenotypic properties as well as those of T cells in other immune organs were examined in DBA/1 mice. Since a significant number of mononuclear cells (MNC) was also yielded by the joints of normal DBA/1 mice, the properties of these T cells were examined in parallel. When CIA was induced by an intradermal injection of type II collagen at the base of the tail, the numbers of MNC yielded by the regional lymph nodes and the foot joints were doubled. Interestingly, regardless of the onset of CIA, the joints were always comprised of unique T cell populations, including IL-2(R)alpha- beta+ T cells, gammadelta T cells, CD8alpha+ beta- cells, and CD44+ L-selectin- cells. All these properties coincide with those of extrathymic T cells in liver and intestine. In the case of gammadelta T cells in joints, Vgamma and Vdelta usages were unique and different from those in the other organs. More importantly, Vgamma and Vdelta usages in gammadelta T cells in the joints of normal mice and in those of mice with CIA were essentially the same. Taken together with the expression of recombination-activating gene-1 and -2 mRNAs by MNC in mice with CIA, these findings raise the possibility that the joints have their own resident T cells that are extrathymically generated in situ.     

多路红外防盗报警器

介绍一种利用热释红外线传感器探头作为探测器的多路防盗报警器.该报警器能够自动完成交直流供电转换,具有灵敏度高、电压适用范围宽、准确可靠等特点,并配有多种声光报警方式.给出了该报警器的基本原理和方框图。     

Adeno-associated virus 2-mediated high efficiency gene transfer into immature and mature subsets of hematopoietic progenitor cells in human umbilical cord blood

Recombinant adeno-associated virus 2 (AAV) virions were constructed containing a gene for resistance to neomycin (neoR), under the control of either the herpesvirus thymidine kinase (TK) gene promoter (vTK-Neo), or the human parvovirus B19 p6 promoter (vB19-Neo), as well as those containing an upstream erythroid cell-specific enhancer (HS-2) from the locus control region of the human beta-globin gene cluster (vHS2-TK-Neo; vHS2-B19-Neo). These recombinant virions were used to infect either low density or highly enriched populations of CD34+ cells isolated from human umbilical cord blood. In clonogenic assays initiated with cells infected with the different recombinant AAV-Neo virions, equivalent high frequency transduction of the neoR gene into slow-cycling multipotential, erythroid, and granulocyte/macrophage (GM) progenitor cells, including those with high proliferative potential, was obtained without prestimulation with growth factors, indicating that these immature and mature hematopoietic progenitor cells were susceptible to infection by the recombinant AAV virions. Successful transduction did not require and was not enhanced by prestimulation of these cell populations with cytokines. The functional activity of the transduced neo gene was evident by the development of resistance to the drug G418, a neomycin analogue. Individual high and low proliferative colony-forming unit (CFU)-GM, burst-forming unit-erythroid, and CFU-granulocyte erythroid macrophage megakaryocyte colonies from mock-infected, or the recombinant virus-infected cultures were subjected to polymerase chain reaction analysis using a neo-specific synthetic oligonucleotide primer pair. A 276-bp DNA fragment that hybridized with a neo-specific DNA probe on Southern blots was only detected in those colonies cloned from the recombinant virus-infected cells, indicating stable integration of the transduced neo gene. These studies suggest that parvovirus-based vectors may prove to be a useful alternative to the more commonly used retroviral vectors for high efficiency gene transfer into slow or noncycling primitive hematopoietic progenitor cells, without the need for growth factor stimulation, which could potentially lead to differentiation of these cells before transplantation.