RACE技术对薯蓣皂苷糖苷酶基因cDNA 3'末端的快速扩增

对微生物Absidia sp.G3d产薯蓣皂苷糖苷酶基因cDNA 3'末端的序列进行了调取.根据薯蓣皂苷糖苷酶基因的CDS区已知序列设计特异性引物,应用3'RACE(Rapid-Amplification of eDNA 3'Ends)技术,快速扩增得到cDNA 3'端未知序列片段,将PCR产物切胶回收,直接测序得到长度为258 bp的产物序列,经比对其中编码区长度为122 bp,非编码区长度为136 bp,Poly A部分长度为28 bp.这对其他类中草药高活性皂苷糖基水解酶基因cDNA3'末端的序列的调取提供了参考.     

传统建筑意趣的现代诠释

文章结合杭州西溪新天地的设计分析,从空间序列的建构、文化氛围的建构以及其中人员活动的建构等方面,讨论了现代开发方式与传统街坊理念之间的平衡之道。     

Dynamic construction site layout planning using max-min ant system

Construction site layout planning (CSLP) is a dynamic multi-objective optimization (MOO) problem as there are different facilities employed in the different construction phases of a construction project. In this study, a new method using continuous dynamic searching scheme to guide the max-min ant system (MMAS) algorithm, which is one of the ant colony optimization (ACO) algorithms, to solve the dynamic CSLP problem under the two congruent objective functions of minimizing safety concerns and reducing construction cost is proposed. Using weighted sum method the MOO problem can be solved by the proposed MMAS method. An office building case was used to verify the capability of the proposed method to solve dynamic CSLP problem and the results are promising. The approach could be benchmarked by researchers using other advanced optimization algorithms to solve the same problem or expand the applications to other fields.     

经济型N80油管热处理工艺研究

为降低N80钢的生产成本,开发了一种经济型钢种,通过回火试验及力学性能试验,制订了该钢的热处理工艺.结果表明该钢的力学性能超过了API 5CTN80钢级的要求.     

电信集中结算系统高可用建设研究

本文提出了电信集中结算高可用系统的基本概念．详细介绍了集中结算系统高可用性方面的建设方案，然后对集中结算系统在某省的运行情况进行了分析。     

一种基于DSP的H.264到H.263实时转码器

设计了一种基于DSP的H.264到H.263实时转码器,充分利用视频编解码过程的冗余信息,对传统结构的转码器进行改进,降低了转码运算复杂度.结合TMS320DM642的特点在DSP平台上完成了这两种转码器的优化实现.最终实验数据表明,传统结构转码器优化后转码速度能达到20f/s(帧/秒),而所设计的转码器则能实现CIF格式25f/s从H.264到H.263的实时转码,并且没有显著的视频质量损失.     

一种自适应的混合型无线NoC拓扑结构

针对传统大规模片上网络（Network-on-Chip,NoC）远距离核间多跳通信所带来的高能耗与延时问题,提出了一种基于虚Torus的自适应的混合型无线NoC拓扑结构（VT-AWiNoC）．该结构通过引入链路拥塞测度作为感知参数,基于此采用热点无线链路自动探测与带宽动态分配机制,并设计实现发送器动态分配的控制电路模块,以达到根据不同的通信流量模型,于片内自适应地调整拓扑结构及链路带宽的目的．通过建立混合型无线NoC的延时与功耗评估模型,对该结构的无线NoC进行性能评估．实验结果表明,该自适应拓扑与其它混合型无线NoC相比,在随机流量模型下,网络平均延时降低了16．52～23．27％;在20％的热点流量模型下,包平均能耗节省了39．19％;以真实应用FFT作为基准测试,平均延时降低了17．20％～21．68％,并节省了23．49％的包平均能耗．该结构以较小的面积开销获得了更优的性能．     

Lightweight Triboelectric Nanogenerator for Energy Harvesting and Sensing Tiny Mechanical Motion

Triboelectric nanogenerators (TENGs) have shown exciting applications in mechanical energy harvesting and self‐powered sensing. Aiming at commercial applications, cost reduction and simplification of TENG structures are of great interest. In this work, a lightweight TENG based on the integration of polymer nanowires and a carbon sponge, which serves both as the substrate and an electrode, are reported. Because of the low density of the carbon sponge and the filmy nanowires, the device exhibits a total mass of less than 0.1 g for a volume of 12.5 cm³ and it produces a short‐circuit current of 6 μA, open‐circuit voltage of 75 V, and a maximum output power of 0.28 W kg^?1 under light finger tapping. The device can linearly measure the acceleration at a detection limit down to 0.25 m s^?2 and for a detection range from 0.25 m s^?2 to 10.0 m s^?2.     

一种加权均方误差最小化的鲁棒性干扰对齐算法

线性干扰对齐的一个常见优化目标是总传输速率最大化,但因为和速率函数的非凸特性而难以直接求解。加权均方误差最小化算法借助均方误差与和速率之间的等价关系解决了这一问题。这一方法需要获得准确的信道状态信息,在实际应用中,通道估计误差的存在会导致算法性能的下降。该文提出一种改进算法,在干扰对齐预编码矩阵与接收矩阵的优化求解过程中将通道估计误差的统计特性考虑在内。仿真结果表明,相比以往的加权均方误差最小化算法,该文算法对信道估计误差具有较高的鲁棒性,可以有效提高总的传输速率。     

Reinforced Wool Keratin Fibers via Dithiol Chain Re-bonding

Regenerated wool keratin fibers (RWKFs) have heretofore attracted tremendous interest according to environmental friendliness, ample resource, and intrinsic biocompatibility for broad applications. In this realm, both uncontrollable keratin fibril assembly procedure and resultant insufficient mechanical strength, have greatly hindered their large-scale manufacture and commercial viability. Herein, a continuous wet-spinning strategy is put forward to rebuild wool keratin into compact regenerated bio-fibers with improved strength via disulfide re-bonding. Dithiothreitol (DTT) has been introduced to renovate disulfide linkage inside keratin polypeptide chains, and bridge keratin fibrils via covalent thiol bonding to form a continuous backbone as mechanical support. A thus-derived RWKF manifests a tensile strength of 186.1 ± 7.0 MPa and Young's modulus of 7.4 ± 0.2 GPa, which exceeds those of natural wool, feathers, and regenerated wool or feather keratin fibers. The detailed wet-spinning technical parameters, such as coagulation, oxidation, and post-treatment, have been systematically optimized to guarantee the continuous preparation of high-strength regenerated keratin fibers. This work offers insight into solving the concurrent challenges for continuous manufacture of regenerated protein fibers and sustainability concerns about biomass waste.