And Yet It Moves: Oxidation of the Nuclear Autoantigen La/SS-B Is the Driving Force for Nucleo-Cytoplasmic Shuttling

Decades ago, we and many other groups showed a nucleo-cytoplasmic translocation of La protein in cultured cells. This shuttling of La protein was seen after UV irradiation, virus infections, hydrogen peroxide exposure and the Fenton reaction based on iron or copper ions. All of these conditions are somehow related to oxidative stress. Unfortunately, these harsh conditions could also cause an artificial release of La protein. Even until today, the shuttling and the cytoplasmic function of La/SS-B is controversially discussed. Moreover, the driving mechanism for the shuttling of La protein remains unclear. Recently, we showed that La protein undergoes redox-dependent conformational changes. Moreover, we developed anti-La monoclonal antibodies (anti-La mAbs), which are specific for either the reduced form of La protein or the oxidized form. Using these tools, here we show that redox-dependent conformational changes are the driving force for the shuttling of La protein. Moreover, we show that translocation of La protein to the cytoplasm can be triggered in a ligand/receptor-dependent manner under physiological conditions. We show that ligands of toll-like receptors lead to a redox-dependent shuttling of La protein. The shuttling of La protein depends on the redox status of the respective cell type. Endothelial cells are usually resistant to the shuttling of La protein, while dendritic cells are highly sensitive. However, the deprivation of intracellular reducing agents in endothelial cells makes endothelial cells sensitive to a redox-dependent shuttling of La protein.     

永乐小区住宅工程质量控制

介绍了住宅工程的质量管理和创优工程分部分项的质量控制。     

Children's residential exposure to chlorpyrifos: application of CPPAES field measurements of chlorpyrifos and TCPy within MENTOR/SHEDS-Pesticides model

The comprehensive individual field-measurements on non-dietary exposure collected in the Children's-Post-Pesticide-Application-Exposure-Study (CPPAES) were used within MENTOR/SHEDS-Pesticides, a physically based stochastic human exposure and dose model. In this application, however, the model was run deterministically. The MENTOR/SHEDS-Pesticides employed the CPPAES as input variables to simulate the exposure and the dose profiles for seven children over a 2-week post-application period following a routine residential and professional indoor crack-and-crevice chlorpyrifos application. The input variables were obtained from a personal activity diary, microenvironmental measurements and personal biomonitoring data obtained from CPPAES samples collected from the individual children and in their homes. Simulation results were compared with CPPAES field measured values obtained from the children's homes to assess the utility of the different microenvironmental data collected in CPPAES, i.e. indicator toys and wipe samplers to estimate aggregate exposures that can be result from one or more exposure pathways and routes. The final analyses of the database involved comparisons of the actual data obtained from the individual biomarker samples of a urinary metabolite of chlorpyrifos (TCPy) and the values predicted by MENTOR/SHEDS-Pesticides using the CPPAES-derived variables. Because duplicate diet samples were not part of the CPPAES study design, SHEDs-Pesticides simulated dose profiles did not account for the dietary route. The research provided more confidence in the types of data that can be used in the inhalation and dermal contact modules of MENTOR/SHEDS-Pesticides to predict the pesticide dose received by a child. It was determined that we still need additional understanding about: (1) the types of activities and durations of activities that result in non-dietary ingestion of pesticides and (2) the influence of dietary exposures on the levels of TCPy found in the urine.     

基于Kinect的人体作业仿真方法研究

为了提高生产企业作业分析的效率和改善作业,提出了一种基于非介入式运动捕捉设备Kinect的人体作业仿真方法。首先利用Kinect对真实的人体作业动作进行捕获,获取所需的人体运动数据,接着在Kinect人体模型和人机工效软件DELMIA的人体模型之间建立映射,并在此基础上对DELMIA软件进行二次开发,最终实现由Kinect获取的人体运动数据驱动DELMIA里的虚拟人体进行仿真作业。仿真结果表明：基于Kinect的作业仿真方法在运动捕捉的适用环境、精确性和响应速度等方面都能很好地满足企业应用的要求。     

基于比值法的铁磁谐振过电压分类研究

在变电站实测过电压波形基础上,根据铁磁谐振过电压发生机理及波形特征,将小波变换良好的时频局部化特性和傅里叶变换良好的频域分析能力有机结合,提取了能够有效反映铁磁谐振过电压的特征参量,并提出利用比值法识别铁磁谐振过电压的方法。结果表明该理论基础直观,大量现场实测数据验证了该方法在识别铁磁谐振过电压时的有效性和可靠性。     

基于网络流技术实现Intranent蠕虫快速侦测

分析了蠕虫病毒的主要特征。介绍了两种主要的网络流技术NetFlow和sFlow的优缺点以及在蠕虫病毒侦测中的应用方式，设计实现了一套兼容两种技术的Intranet蠕虫病毒快速侦测系统，重点介绍了蠕虫病毒侦测系统的系统结构和主要关键技术。     

Metabotropic glutamate receptor in vasopressin, CRF and VIP hypothalamic neurones

The excitatory amino acid glutamate, acting via ionotropic and metabotropic glutamate receptors, appears to play an important role in the control of neuroendocrine functions. The aim of the present investigations was to determine whether hypothalamic neurones which synthesize arginin-vasopressin (AVP), CRF and VIP express metabotropic glutamate receptor (mGluR). Double-label immunocytochemistry and the mirror technique were used. We found that AVP immunoreactive neurones of the paraventricular, supraoptic and suprachiasmatic nuclei contain mGluR1a, but the number of double-labelled neurones was different in the three cell groups. mGluR1a was present in a significant number of paraventricular CRF nerve cells, and in almost all VIP neurones of the SCHN. These results support the view that the excitatory transmitter glutamate may directly influence AVP, CRF and VIP neurones of the three hypothalamic cell groups.     

Neuropsychologists' capacity to detect adolescent malingerers.

Malingering on neuropsychological testing is little studied but increasingly relevant, given the growth of forensic neuropsychology. We performed a two-part study on neuropsychologists' capacity to detect adolescent simulators. Three teenagers were instructed to "fake bad" on neuropsychological testing. In Study 1, test results and a fabricated history of mild to moderate head injury were sent to a representative sample of neuropsychologists, each of whom reviewed one case. About three fourths of the respondents judged the cases to be abnormal and attributed results to cortical dysfunction; no respondent detected malingering. In Study 2, we examined the effects of forewarning on case appraisal. An equal number of malingerers' cases and actual cases were sent to a new sample of neuropsychologists, who were informed of the 50% base rate for malingering in the study. Although respondents were generally quite confident in their case appraisals, detection of malingering did not surpass chance level. The results of this and other studies suggest that adults and adolescents can fake convincing deficits on neuropsychological testing. Clinicians' overconfidence in their ability to detect simulation may partly explain why so little research has been devoted to this topic. (PsycINFO Database Record (c) 2010 APA, all rights reserved)